The EVES motif mediates both intermolecular and intramolecular regulation of c-Myb.
10.1101/gad.10.15.1858
Crossref journal-article
Cold Spring Harbor Laboratory
Genes & Development (246)
Abstract

The c-Myb transcription factor is a proto-oncoprotein whose latent transforming activity can be unmasked by truncation of either terminus. Because both ends of Myb are involved in negative regulation, we tested whether they could associate in a two-hybrid assay and identified a carboxy-terminal motif that interacts with the amino-terminal DNA-binding domain. The EVES motif is highly conserved in vertebrate c-Myb proteins and contains a known site of phosphorylation previously implicated in the negative regulation of c-Myb. Interestingly, a related EVES motif is present in p100, a ubiquitously expressed transcriptional coactivator found in diverse species. We show that p100 interacts with and influences the activity of c-Myb, implicating it in the regulation of c-Myb, differentiation, and cell growth. Our results suggest that Myb is regulated by a novel mechanism in which intramolecular interactions and conformational changes control the intermolecular associations among Myb, p100, and the transcriptional apparatus.

Bibliography

Dash, A. B., Orrico, F. C., & Ness, S. A. (1996). The EVES motif mediates both intermolecular and intramolecular regulation of c-Myb. Genes & Development, 10(15), 1858–1869.

Authors 3
  1. A B Dash (first)
  2. F C Orrico (additional)
  3. S A Ness (additional)
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Dates
Type When
Created 18 years, 2 months ago (June 5, 2007, 5:15 p.m.)
Deposited 3 years, 9 months ago (Nov. 14, 2021, 9:54 p.m.)
Indexed 1 year, 1 month ago (July 17, 2024, 5:40 a.m.)
Issued 29 years, 1 month ago (Aug. 1, 1996)
Published 29 years, 1 month ago (Aug. 1, 1996)
Published Online 29 years, 1 month ago (Aug. 1, 1996)
Published Print 29 years, 1 month ago (Aug. 1, 1996)
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@article{Dash_1996, title={The EVES motif mediates both intermolecular and intramolecular regulation of c-Myb.}, volume={10}, ISSN={1549-5477}, url={http://dx.doi.org/10.1101/gad.10.15.1858}, DOI={10.1101/gad.10.15.1858}, number={15}, journal={Genes & Development}, publisher={Cold Spring Harbor Laboratory}, author={Dash, A B and Orrico, F C and Ness, S A}, year={1996}, month=aug, pages={1858–1869} }