DOI: 10.1101/gad.10.12.1503. Xe-p9, a Xenopus Suc1/Cks homolog, has multiple essential roles in cell cycle control.

Xe-p9, a Xenopus Suc1/Cks homolog, has multiple essential roles in cell cycle control.

10.1101/gad.10.12.1503

Crossref journal-article

Cold Spring Harbor Laboratory

Genes & Development (246)

Abstract

The small Suc1/Cks protein is a ubiquitous subunit of Cdk/cyclin complexes, but its precise function has remained unclear. We have isolated a Xenopus homolog, Xe-p9, of the Suc1/Cks protein by virtue of its ability to rescue a fission yeast mutant that enters mitosis prematurely. To assess its functional role in cell cycle control, we have both overexpressed p9 in Xenopus egg extracts and immunodepleted the protein from these extracts. We found that addition of recombinant His6-p9 to egg extracts results in a pronounced delay of mitosis that can be attributed to an inhibition of the tyrosine dephosphorylation of the inactive Cdc2/cyclin B complex. In immunodepletion studies, we observed that the consequences of removing p9 from egg extracts depend on the stage of the cell cycle. Specifically, in the case of interphase extracts, the removal of p9 abolishes the entry into mitosis as a result of a failure in the activation of the Cdc2/cyclin B complex by tyrosine dephosphorylation. Furthermore, mitotic extracts lacking p9 fail to exit mitosis because of a defect in the destruction of cyclin B. Collectively, these results indicate that p9 has multiple essential roles in the cell cycle by governing the interaction of the Cdc2/cyclin B complex with both positive and negative regulators.

Bibliography

Patra, D., & Dunphy, W. G. (1996). Xe-p9, a Xenopus Suc1/Cks homolog, has multiple essential roles in cell cycle control. Genes & Development, 10(12), 1503â1515.

Authors 2

D Patra (first)
W G Dunphy (additional)

References 69 Referenced 76

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{'key': '2021111418375969000_10.12.1503.24', 'first-page': '2034', 'article-title': 'The Saccharomyces cerevisiae CKS1 gene, a homolog of the Schizosaccharomyces pombe suc1+\n gene, encodes a subunit of the Cdc28 protein kinase complex.', 'volume': '9', 'year': '1989', 'journal-title': 'Mol. Cell. Biol.'} / Mol. Cell. Biol. / The Saccharomyces cerevisiae CKS1 gene, a homolog of the Schizosaccharomyces pombe suc1+ gene, encodes a subunit of the Cdc28 protein kinase complex. (1989)
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Dates

Type	When
Created	18 years, 2 months ago (June 5, 2007, 5:15 p.m.)
Deposited	3 years, 9 months ago (Nov. 14, 2021, 9:50 p.m.)
Indexed	1 month, 2 weeks ago (July 9, 2025, 6:54 p.m.)
Issued	29 years, 2 months ago (June 15, 1996)
Published	29 years, 2 months ago (June 15, 1996)
Published Online	29 years, 2 months ago (June 15, 1996)
Published Print	29 years, 2 months ago (June 15, 1996)

Funders 0

None

BibTeX

@article{Patra_1996, title={Xe-p9, a Xenopus Suc1/Cks homolog, has multiple essential roles in cell cycle control.}, volume={10}, ISSN={1549-5477}, url={http://dx.doi.org/10.1101/gad.10.12.1503}, DOI={10.1101/gad.10.12.1503}, number={12}, journal={Genes & Development}, publisher={Cold Spring Harbor Laboratory}, author={Patra, D and Dunphy, W G}, year={1996}, month=jun, pages={1503–1515} }

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