DOI: 10.1101/gad.1.5.471. Relative position and strengths of poly(A) sites as well as transcription termination are critical to membrane versus secreted mu-chain expression during B-cell development.

Relative position and strengths of poly(A) sites as well as transcription termination are critical to membrane versus secreted mu-chain expression during B-cell development.

10.1101/gad.1.5.471

Crossref journal-article

Cold Spring Harbor Laboratory

Genes & Development (246)

Abstract

During B-cell differentiation, there is a dramatic switch in the RNA products of the immunoglobulin mu heavy chain transcription unit. In the mature B cell there is roughly equal production of the microseconds and the micron RNA, whereas in the antibody-secreting plasma cell there is nearly exclusive production of the microseconds RNA. A plasmid containing the entire mu transcription unit was properly regulated when assayed by transient transfection in a B lymphoma and a plasmacytoma. In contrast, no such regulation was observed with separate plasmids that could produce only one or the other RNA. Instead, the micron poly(A) site was utilized more efficiently than the microseconds poly(A) site, irrespective of the cell type. We also found that transcription termination prior to the micron poly(A) site in plasmacytomas contributes to preferential production of microseconds RNA in these cells. Finally, reducing the distance between the two poly(A) sites improved the use of the micron site at the expense of the use of the microseconds in B lymphoma cells, suggesting a competition for a limiting factor. Such competition was not apparent in plasmacytomas. We conclude that relative poly(A) site strength and the position of the poly(A) sites within the transcription unit, coupled with a changing concentration of a limiting factor, as well as transcription termination prior to the micron poly(A) site, all play a role in determining the expression of the mu locus during B-cell development.

Bibliography

Galli, G., Guise, J. W., McDevitt, M. A., Tucker, P. W., & Nevins, J. R. (1987). Relative position and strengths of poly(A) sites as well as transcription termination are critical to membrane versus secreted mu-chain expression during B-cell development. Genes & Development, 1(5), 471â481.

Authors 5

G Galli (first)
J W Guise (additional)
M A McDevitt (additional)
P W Tucker (additional)
J R Nevins (additional)

References 31 Referenced 68

10.1016/0092-8674(80)90615-7
{'key': '2021111319421717000_1.5.471.2', 'first-page': '386', 'article-title': 'Multiple immunoglobulin heavy chain gene transcripts in Abelson murine leukemia virus transformed lymphoid cell lines.', 'volume': '2', 'year': '1982', 'journal-title': 'Mol. Cell. Biol.'} / Mol. Cell. Biol. / Multiple immunoglobulin heavy chain gene transcripts in Abelson murine leukemia virus transformed lymphoid cell lines. (1982)
10.1016/S0092-8674(85)80007-6
10.1038/307417a0
10.1021/bi00591a005
10.1073/pnas.82.24.8658
{'key': '2021111319421717000_1.5.471.7', 'first-page': '313', 'article-title': 'Two mRNAs can be produced from a single immunoglobulin Âµ gene by alternative RNA processing pathways.', 'volume': '20', 'year': '1979', 'journal-title': 'Cell'} / Cell / Two mRNAs can be produced from a single immunoglobulin Âµ gene by alternative RNA processing pathways. (1979)
10.1016/0092-8674(85)90349-6
10.1016/0092-8674(86)90536-2
10.1016/S0092-8674(85)80069-6
10.1016/0092-8674(81)90154-9
10.1093/nar/7.6.1541
10.1093/nar/14.13.5431
10.1002/eji.1830110605 / Eur. J. Immunol. / Membrane immunoglobulins of spontaneous B-lymphomas of aged BALB/c mice. (1981)
10.1038/318475a0
10.1016/0092-8674(84)90226-5
{'key': '2021111319421717000_1.5.471.17', 'first-page': '2514', 'article-title': 'Cell-specific expression of secreted versus membrane forms of immunoglobulin gamma 2b mRNA involves selective use of alternate polyadenylation sites.', 'volume': '5', 'year': '1985', 'journal-title': 'Mol. Cell. Biol.'} / Mol. Cell. Biol. / Cell-specific expression of secreted versus membrane forms of immunoglobulin gamma 2b mRNA involves selective use of alternate polyadenylation sites. (1985)
{'key': '2021111319421717000_1.5.471.18', 'first-page': '1317', 'article-title': 'Characterization of productive and sterile transcripts from the immunoglobulin heavy-chain locus: Processing of Âµm and Âµs mRNA.', 'volume': '3', 'year': '1983', 'journal-title': 'Mol. Cell. Biol.'} / Mol. Cell. Biol. / Characterization of productive and sterile transcripts from the immunoglobulin heavy-chain locus: Processing of Âµm and Âµs mRNA. (1983)
10.1016/0022-2836(79)90436-4
10.1146/annurev.bi.52.070183.002301
10.1016/0092-8674(78)90071-5
10.1038/290113a0
10.1073/pnas.80.3.825
10.1146/annurev.bi.55.070186.005351
10.1016/0092-8674(79)90243-5
10.1073/pnas.83.23.8883
10.1016/0092-8674(80)90616-9
{'key': '2021111319421717000_1.5.471.28', 'first-page': '123', 'article-title': 'Cell-type-specific synthesis of murine immunoglobulin Âµ RNA from an adenovirus vector.', 'volume': '6', 'year': '1986', 'journal-title': 'Mol. Cell. Biol.'} / Mol. Cell. Biol. / Cell-type-specific synthesis of murine immunoglobulin Âµ RNA from an adenovirus vector. (1986)
10.1016/0092-8674(77)90134-9
10.1002/j.1460-2075.1986.tb04587.x / EMBO J. / Î±-Thalassaemia caused by a poly(A) site mutation reveals that transcriptional termination is linked to 3â² end processing in the human Î±2 globin gene. (1986)
10.1084/jem.160.2.564

Dates

Type	When
Created	18 years, 2 months ago (June 5, 2007, 5:15 p.m.)
Deposited	3 years, 9 months ago (Nov. 13, 2021, 10:43 p.m.)
Indexed	5 months ago (March 31, 2025, 7:02 a.m.)
Issued	38 years, 2 months ago (July 1, 1987)
Published	38 years, 2 months ago (July 1, 1987)
Published Online	38 years, 2 months ago (July 1, 1987)
Published Print	38 years, 2 months ago (July 1, 1987)

Funders 0

None

BibTeX

@article{Galli_1987, title={Relative position and strengths of poly(A) sites as well as transcription termination are critical to membrane versus secreted mu-chain expression during B-cell development.}, volume={1}, ISSN={1549-5477}, url={http://dx.doi.org/10.1101/gad.1.5.471}, DOI={10.1101/gad.1.5.471}, number={5}, journal={Genes & Development}, publisher={Cold Spring Harbor Laboratory}, author={Galli, G and Guise, J W and McDevitt, M A and Tucker, P W and Nevins, J R}, year={1987}, month=jul, pages={471–481} }

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