DOI: 10.1101/gad.1.3.247. Sequences in the pea rbcS-3A gene have homology to constitutive mammalian enhancers but function as negative regulatory elements.

Sequences in the pea rbcS-3A gene have homology to constitutive mammalian enhancers but function as negative regulatory elements.

10.1101/gad.1.3.247

Crossref journal-article

Cold Spring Harbor Laboratory

Genes & Development (246)

Abstract

The pea rbcS-3A gene, which codes for a key component of the photosynthetic machinery, requires light for its expression. Analysis of chimeric constructs in transgenic tobacco plants has shown that a 280-bp fragment from the 5' noncoding region can act as a light-inducible transcriptional enhancer. Further characterization of this enhancer identifies a 58-bp sequence containing two regulatory elements that can decrease transcription in the dark. One has a high degree of homology to the SV40 core enhancer, the other to the adenovirus 5 E1A enhancer and the constitutive part of the human interferon-beta gene enhancer. Deletion of the 58-bp sequence uncovers additional light-responsive elements (LREs) located further upstream and downstream.

Bibliography

Kuhlemeier, C., Fluhr, R., Green, P. J., & Chua, N. H. (1987). Sequences in the pea rbcS-3A gene have homology to constitutive mammalian enhancers but function as negative regulatory elements. Genes & Development, 1(3), 247â255.

Authors 4

C Kuhlemeier (first)
R Fluhr (additional)
P J Green (additional)
N H Chua (additional)

References 35 Referenced 102

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10.1093/nar/11.6.1645
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10.1002/j.1460-2075.1986.tb04467.x / EMBO J. / Expression dynamics of the pea rbcS family and organ distribution of the transcripts. (1986)
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Gluzman, Y. 1985. Eukaryotic transcription: The role of cis and trans-acting elements in initiation. Cold Spring Harbor Laboratory, Cold Spring Harbor, New York.
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10.1016/0092-8674(86)90292-8
Hearing, P. and T. Shenk. 1985. Adenovirus enhancer elements. In Eukaryotic transcription (ed. Y. Gluzman), p. 48. Cold Spring Harbor Laboratory, Cold Spring Harbor, New York.
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Inouye, S. and M. Inouye. 1986. Oligonucleotide-directed site-specific mutagenesis using double-stranded plasmid DNA. In Synthesis of DNA, RNA and their applications (ed. S. Narang), p. 181. Academic Press, New York. (10.1016/B978-0-12-514030-0.50013-X)
10.1002/j.1460-2075.1985.tb03949.x / EMBO J. / High level expression of introduced chimaeric genes in regenerated transformed plants. (1985)
10.1073/pnas.83.10.3151
Lerbs, S., W. Lerbs, R. Wollgiehn, and B. Parthier. 1985. Hormone (cytokinin) and light effects in RUBISCO gene expression. In Molecular form and function of the plant genome (ed. L. van Vloten-Doting, G.S.P. Groot, and T.C. Hall), p. 267. Plenum, New York.
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10.1002/j.1460-2075.1986.tb04278.x / EMBO J. / Several hundred base pairs upstream of Drosophila hsp23 and 26 are required for their heat induction in transformed flies. (1986)
{'key': '2021111319413641000_1.3.247.26', 'first-page': '31', 'article-title': 'Activation of heat-shock genes in eukaryotes.', 'volume': '2', 'year': '1986', 'journal-title': 'Trends Genet.'} / Trends Genet. / Activation of heat-shock genes in eukaryotes. (1986)
10.1002/j.1460-2075.1982.tb01340.x / EMBO J. / A synthetic heat-shock promoter element confers heat-inducibility on the herpes simplex virus thymidine kinase gene. (1982)
10.1016/0168-9525(86)90233-7
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10.1104/pp.79.2.349
10.1073/pnas.81.23.7318
10.1038/318579a0
10.1016/0968-0004(86)90031-9
10.1002/j.1460-2075.1986.tb04224.x / EMBO J. / Multiple sequence motifs are involved in SV40 enhancer function. (1986)
10.1016/0092-8674(83)90544-5

Dates

Type	When
Created	18 years, 2 months ago (June 5, 2007, 5:15 p.m.)
Deposited	3 years, 9 months ago (Nov. 13, 2021, 10:41 p.m.)
Indexed	4 weeks, 2 days ago (Aug. 5, 2025, 8:47 a.m.)
Issued	38 years, 4 months ago (May 1, 1987)
Published	38 years, 4 months ago (May 1, 1987)
Published Online	38 years, 4 months ago (May 1, 1987)
Published Print	38 years, 4 months ago (May 1, 1987)

Funders 0

None

BibTeX

@article{Kuhlemeier_1987, title={Sequences in the pea rbcS-3A gene have homology to constitutive mammalian enhancers but function as negative regulatory elements.}, volume={1}, ISSN={1549-5477}, url={http://dx.doi.org/10.1101/gad.1.3.247}, DOI={10.1101/gad.1.3.247}, number={3}, journal={Genes & Development}, publisher={Cold Spring Harbor Laboratory}, author={Kuhlemeier, C and Fluhr, R and Green, P J and Chua, N H}, year={1987}, month=may, pages={247–255} }

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