DOI: 10.1101/gad.1.2.161. Species- and tissue-specific expression of human alpha 1-antitrypsin in transgenic mice.

Species- and tissue-specific expression of human alpha 1-antitrypsin in transgenic mice.

10.1101/gad.1.2.161

Crossref journal-article

Cold Spring Harbor Laboratory

Genes & Development (246)

Abstract

alpha 1-Antitrypsin (alpha 1AT) is an abundant serum protein whose major site of synthesis is in the hepatocyte. alpha 1AT transcripts are also present, albeit at a lower level, in a variety of other human tissues. This pattern of expression is partly related to initiation of transcription at sites with distinct tissue specificities. The mouse alpha 1AT gene, in contrast, is more strictly liver specific in its expression. To explore the regulation of the alpha 1AT gene we have microinjected a cosmid insert carrying the human gene into fertilized mouse eggs. In three lines obtained from transgenic mice, inheritance of copies of the human gene is accompanied by a high serum concentration of the human protein. Human alpha 1AT RNA accumulates to the highest level in liver of transgenic animals. The presence of transcripts in other tissues indicates that the human pattern of expression is maintained, whereas the temporal activity of the introduced gene parallels that of the endogenous one during mouse embryogenesis.

Bibliography

Kelsey, G. D., Povey, S., Bygrave, A. E., & Lovell-Badge, R. H. (1987). Species- and tissue-specific expression of human alpha 1-antitrypsin in transgenic mice. Genes & Development, 1(2), 161â171.

Authors 4

G D Kelsey (first)
S Povey (additional)
A E Bygrave (additional)
R H Lovell-Badge (additional)

References 41 Referenced 91

10.1073/pnas.79.2.500
10.1016/S0021-9258(20)71222-6 / J. Biol. Chem. / An evolutionary switch in tissue-specific gene expression: Abundant expression of α1-antitrypsin in the kidney of a wild mouse species. (1985)
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10.1038/314377a0
10.1016/S0092-8674(85)80026-X
10.1073/pnas.79.3.870
10.1016/0012-1606(86)90231-9
{'key': '2021111319410788000_1.2.161.8', 'first-page': '1', 'article-title': 'The Pi polymorphism: Genetic, biochemical, and clinical aspects of human α1-antitrypsin.', 'volume': '11', 'year': '1981', 'journal-title': 'Adv. Hum. Genet.'} / Adv. Hum. Genet. / The Pi polymorphism: Genetic, biochemical, and clinical aspects of human α1-antitrypsin. (1981)
10.1172/JCI106109 / J. Clin. Invest. / Development of γG, γA, γM, β1C/β1A, C′1 esterase inhibitor, ceruloplasmin, transferrin, hemopexin, haptoglobin, fibrinogen, plasminogen, α1-antitrypsin, orosomucoid, β-lipoprotein, α2-macroglobulin, and prealbumin in the human conceptus. (1969)
10.1038/228995a0
{'key': '2021111319410788000_1.2.161.11', 'first-page': '477', 'article-title': 'Multiple regulatory elements in the intergenic region between the α-fetoprotein and albumin genes.', 'volume': '6', 'year': '1986', 'journal-title': 'Mol. Cell. Biol.'} / Mol. Cell. Biol. / Multiple regulatory elements in the intergenic region between the α-fetoprotein and albumin genes. (1986)
10.1016/0092-8674(84)90259-9
10.1126/science.2432657
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{'key': '2021111319410788000_1.2.161.15', 'first-page': '2114', 'article-title': 'A genetic locus closely linked to a protease inhibitor gene complex controls the level of multiple RNAs.', 'volume': '5', 'year': '1985', 'journal-title': 'Mol. Cell. Biol.'} / Mol. Cell. Biol. / A genetic locus closely linked to a protease inhibitor gene complex controls the level of multiple RNAs. (1985)
Hogan, B., F. Costantini, and E. Lacy. 1986. Manipulating the mouse embryo: A laboratory manual. Cold Spring Harbor Laboratory, Cold Spring Harbor, New York.
10.1016/0014-5793(76)80478-4
10.1089/dna.1986.5.29 / DNA / Isolation and characterization of the α1-antitrypsin gene of mice. (1986)
{'key': '2021111319410788000_1.2.161.19', 'first-page': '1639', 'article-title': 'Developmental regulation of α-fetoprotein genes in transgenic mice.', 'volume': '5', 'year': '1985', 'journal-title': 'Mol. Cell. Biol.'} / Mol. Cell. Biol. / Developmental regulation of α-fetoprotein genes in transgenic mice. (1985)
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Lovell-Badge, R.H. 1986. Introduction of genes into embryonic stem cells. In Embryonic stem cells: A practical approach (ed. E. Robertson). IRL Press, Oxford. (In press.)
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10.1002/j.1460-2075.1984.tb02062.x / EMBO J. / Pattern of serum protein gene expression in mouse visceral yolk sac and foetal liver. (1984)
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{'key': '2021111319410788000_1.2.161.38', 'first-page': '551', 'article-title': 'Synthesis of α1-anti-trypsin by human monocytes.', 'volume': '51', 'year': '1983', 'journal-title': 'Clin. Exp. Immunol.'} / Clin. Exp. Immunol. / Synthesis of α1-anti-trypsin by human monocytes. (1983)
10.1002/j.1460-2075.1984.tb02081.x / EMBO J. / Cosmid clones derived from both euchromatic and heterochromatic regions of the human Y chromosome. (1984)
10.1073/pnas.79.18.5661
10.1073/pnas.73.4.1324

Dates

Type	When
Created	18 years, 2 months ago (June 5, 2007, 5:15 p.m.)
Deposited	2 years, 3 months ago (May 12, 2023, 2:23 a.m.)
Indexed	6 minutes ago (Sept. 3, 2025, 5:34 p.m.)
Issued	38 years, 5 months ago (April 1, 1987)
Published	38 years, 5 months ago (April 1, 1987)
Published Online	38 years, 5 months ago (April 1, 1987)
Published Print	38 years, 5 months ago (April 1, 1987)

Funders 0

None

BibTeX

@article{Kelsey_1987, title={Species- and tissue-specific expression of human alpha 1-antitrypsin in transgenic mice.}, volume={1}, ISSN={1549-5477}, url={http://dx.doi.org/10.1101/gad.1.2.161}, DOI={10.1101/gad.1.2.161}, number={2}, journal={Genes & Development}, publisher={Cold Spring Harbor Laboratory}, author={Kelsey, G D and Povey, S and Bygrave, A E and Lovell-Badge, R H}, year={1987}, month=apr, pages={161–171} }

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