The human myc gene family: structure and activity of L-myc and an L-myc pseudogene.
10.1101/gad.1.10.1311
Crossref journal-article
Cold Spring Harbor Laboratory
Genes & Development (246)
Abstract

We have determined the nucleotide sequence and transforming activity of the human L-myc gene and a processed L-myc pseudogene (L-myc psi). We demonstrate by cotransformation assays that a 10.6-kb EcoRI fragment derived from a human placental library contains a complete and functional L-myc gene including transcriptional regulatory sequences sufficient for expression in rat embryo fibroblasts. Organization of the L-myc gene was determined by comparing its sequence to those of the L-myc psi gene and an L-myc cDNA clone derived from a human small cell lung carcinoma. Our results show that L-myc has a three-exon organization similar to that of the c-myc and N-myc genes. The putative L-myc gene product consists of 364 amino acids and contains five of the seven homology regions highly conserved between c-myc and N-myc. These conserved regions are located along the entire length of the putative L-myc protein and are interspersed among nonconserved regions. While the putative L-myc gene product is of a smaller size when compared to the c- and N-myc proteins, the relative positions of certain conserved residues occur in corresponding locations along the peptide backbone of the three proteins. In addition, comparison of the human and murine L-myc gene sequences indicate that the relatively large 5' and 3' untranslated regions are evolutionarily conserved, but that these sequences are totally divergent between the L-, c-, and N-myc genes. Finally, we demonstrate that, like the N- and c-myc genes, the L-myc gene can cooperate with a mutant Ha-ras gene to cause malignant transformation of rat embryo fibroblasts in culture. Our analyses clearly prove that L-myc represents a functional member of the myc oncogene family and further delineate structural features that may be important for the common and divergent functions of the members of this gene family.

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DePinho, R. A., Hatton, K. S., Tesfaye, A., Yancopoulos, G. D., & Alt, F. W. (1987). The human myc gene family: structure and activity of L-myc and an L-myc pseudogene. Genes & Development, 1(10), 1311–1326.

Authors 5
  1. R A DePinho (first)
  2. K S Hatton (additional)
  3. A Tesfaye (additional)
  4. G D Yancopoulos (additional)
  5. F W Alt (additional)
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Dates
Type When
Created 18 years, 2 months ago (June 5, 2007, 5:15 p.m.)
Deposited 3 years, 9 months ago (Nov. 13, 2021, 10:45 p.m.)
Indexed 1 year, 7 months ago (Jan. 27, 2024, 5:36 a.m.)
Issued 37 years, 8 months ago (Dec. 1, 1987)
Published 37 years, 8 months ago (Dec. 1, 1987)
Published Online 37 years, 8 months ago (Dec. 1, 1987)
Published Print 37 years, 8 months ago (Dec. 1, 1987)
Funders 0

None

@article{DePinho_1987, title={The human myc gene family: structure and activity of L-myc and an L-myc pseudogene.}, volume={1}, ISSN={1549-5477}, url={http://dx.doi.org/10.1101/gad.1.10.1311}, DOI={10.1101/gad.1.10.1311}, number={10}, journal={Genes & Development}, publisher={Cold Spring Harbor Laboratory}, author={DePinho, R A and Hatton, K S and Tesfaye, A and Yancopoulos, G D and Alt, F W}, year={1987}, month=dec, pages={1311–1326} }