DOI: 10.1101/gad.1.10.1226. Cytoarchitecture and the patterning of fushi tarazu expression in the Drosophila blastoderm.

Cytoarchitecture and the patterning of fushi tarazu expression in the Drosophila blastoderm.

10.1101/gad.1.10.1226

Crossref journal-article

Cold Spring Harbor Laboratory

Genes & Development (246)

Abstract

In the Drosophila embryo at the blastoderm stage, the segmentation gene fushi tarazu (ftz) is expressed in a seven-banded pattern. The generation of this pattern, like many other segmentation gene expression patterns, coincides with the formation of cell membranes around the blastoderm nuclei. To test the role of cellularization in resolving the banded ftz pattern, we used cytoskeletal inhibitors (colcemid and cytochalasin B) to block cellularization. We found that banded ftz RNA and protein patterns can form without cellular structure. We also tested the importance of rapid degradation of the ftz RNA, using cycloheximide to block degradation. RNA degradation is essential to maintain the banded ftz pattern in a syncytium, but is not required to maintain the pattern in a cellularized embryo. A latticework of cytoskeletal microtubules that forms during cellularization appears to be a key component in localizing the ftz mRNA. We conclude that RNA degradation and cellular structure normally work together to localize ftz RNA to its sites of synthesis.

Bibliography

Edgar, B. A., Odell, G. M., & Schubiger, G. (1987). Cytoarchitecture and the patterning of fushi tarazu expression in the Drosophila blastoderm. Genes & Development, 1(10), 1226â1237.

Authors 3

B A Edgar (first)
G M Odell (additional)
G Schubiger (additional)

References 43 Referenced 84

10.1083/jcb.100.4.1209
10.1016/0092-8674(85)90011-X
10.1016/0092-8674(86)90543-X
10.1016/0092-8674(81)90276-2
{'issue': 'Suppl.', 'key': '2021111319444674000_1.10.1226.5', 'first-page': '197', 'article-title': 'The stability and movement of mRNA in Xenopus oocytes and embryos.', 'volume': '97', 'year': '1986', 'journal-title': 'J. Emhiyol Exp. Morphol.'} / J. Emhiyol Exp. Morphol. / The stability and movement of mRNA in Xenopus oocytes and embryos. (1986)
10.1016/0092-8674(85)90012-1
10.1016/0092-8674(86)90009-7
10.1016/0092-8674(86)90771-3
10.1016/0092-8674(86)90517-9
Ennis, H.L. 1985. Developmental biology of the cellular slime mold. Roche Inst. Mol. Biol. Annu. Rep. 1985 28 â31.
10.1038/313284a0
10.1242/jcs.61.1.31 / J. Cell Sci. / Studies of nuclear and cytoplasmic behavior during the five mitotic cycles that precede gastrulation in Drosophila embryogenesis. (1983)
10.1242/jcs.82.1.155 / J. Cell Sci. / Nuclear antigens follow different pathways into daughter nuclei during mitosis in early Drosophila embryos. (1986)
10.1038/324120a0
10.1016/0012-1606(71)90141-2
10.1016/0092-8674(84)90418-5
10.1126/science.3755551
10.1083/jcb.86.1.212
10.1016/0092-8674(86)90018-8
10.1038/318439a0
10.1002/j.1460-2075.1986.tb04409.x / EMBO J. / Correlative changes in homoeotic and segmentation gene expression in KrÃ¼ppel mutant embryos of Drosophila. (1986)
10.1038/324668a0
10.1083/jcb.102.4.1494
10.1038/321493a0
10.1038/317040a0
10.1016/0092-8674(85)90307-1
10.1038/310025a0
10.1083/jcb.102.6.2015
10.1016/0092-8674(86)90515-5
10.1016/0012-1606(75)90027-5
10.1016/0012-1606(76)90278-5
10.1093/icb/17.3.565 / Am. Zool. / Determination during early embryogenesis of Drosophila melanogaster. (1977)
10.1146/annurev.cb.02.110186.000405
{'key': '2021111319444674000_1.10.1226.34', 'first-page': '179', 'article-title': 'Dynamics of the nuclear envelope and of nuclear pore complexes during mitosis in the Drosophila embryo.', 'volume': '34', 'year': '1984', 'journal-title': 'Eur. J. Cell Biol.'} / Eur. J. Cell Biol. / Dynamics of the nuclear envelope and of nuclear pore complexes during mitosis in the Drosophila embryo. (1984)
10.1038/327383a0
10.1016/0012-1606(76)90070-1
Walter, M. and B.M. Alberts. 1984. Intermediate filaments in tissue culture cells and early embryos of Drosophila melanogaster. In Molecular biology of development (ed. R. Firtel and E. Davidson), pp. 263â272. UCLA Symp. Mol. Cell. Biol. New Series. Alan R. Liss, New York.
10.1016/0014-4827(83)90113-1
{'key': '2021111319444674000_1.10.1226.39', 'first-page': '201', 'article-title': 'Microtubule arrays present during the syncytial and cellular blastoderm stage of the early Drosophila embryo.', 'volume': '163', 'year': '1985', 'journal-title': 'Exp. Cell Res.'} / Exp. Cell Res. / Microtubule arrays present during the syncytial and cellular blastoderm stage of the early Drosophila embryo. (1985)
10.1038/318433a0
Wieschaus, E. and C. NÃ¼sslein-Volhard. 1986. Looking at embryos. In Drosophila: A practical approach (ed. D.B. Roberts), pp. 221â223. IRL Press, Oxford.
10.1083/jcb.82.1.114
{'key': '2021111319444674000_1.10.1226.43', 'first-page': '97', 'article-title': 'Division and migration of nuclei during early embryogenesis of Drosophila melanogaster.', 'volume': '25', 'year': '1976', 'journal-title': 'J. Microsc. Biol. Cell'} / J. Microsc. Biol. Cell / Division and migration of nuclei during early embryogenesis of Drosophila melanogaster. (1976)

Dates

Type	When
Created	18 years, 2 months ago (June 5, 2007, 5:15 p.m.)
Deposited	3 years, 9 months ago (Nov. 13, 2021, 10:50 p.m.)
Indexed	1 month ago (July 31, 2025, 11:47 p.m.)
Issued	37 years, 9 months ago (Dec. 1, 1987)
Published	37 years, 9 months ago (Dec. 1, 1987)
Published Online	37 years, 9 months ago (Dec. 1, 1987)
Published Print	37 years, 9 months ago (Dec. 1, 1987)

Funders 0

None

BibTeX

@article{Edgar_1987, title={Cytoarchitecture and the patterning of fushi tarazu expression in the Drosophila blastoderm.}, volume={1}, ISSN={1549-5477}, url={http://dx.doi.org/10.1101/gad.1.10.1226}, DOI={10.1101/gad.1.10.1226}, number={10}, journal={Genes & Development}, publisher={Cold Spring Harbor Laboratory}, author={Edgar, B A and Odell, G M and Schubiger, G}, year={1987}, month=dec, pages={1226–1237} }

JSON

{
  "indexed": {
    "date-parts": [
      [
        2025,
        8,
        1
      ]
    ],
    "date-time": "2025-08-01T03:47:33Z",
    "timestamp": 1754020053037
  },
  "reference-count": 43,
  "publisher": "Cold Spring Harbor Laboratory",
  "issue": "10",
  "content-domain": {
    "domain": [],
    "crossmark-restriction": false
  },
  "published-print": {
    "date-parts": [
      [
        1987,
        12
      ]
    ]
  },
  "abstract": "<jats:p>In the Drosophila embryo at the blastoderm stage, the segmentation gene fushi tarazu (ftz) is expressed in a seven-banded pattern. The generation of this pattern, like many other segmentation gene expression patterns, coincides with the formation of cell membranes around the blastoderm nuclei. To test the role of cellularization in resolving the banded ftz pattern, we used cytoskeletal inhibitors (colcemid and cytochalasin B) to block cellularization. We found that banded ftz RNA and protein patterns can form without cellular structure. We also tested the importance of rapid degradation of the ftz RNA, using cycloheximide to block degradation. RNA degradation is essential to maintain the banded ftz pattern in a syncytium, but is not required to maintain the pattern in a cellularized embryo. A latticework of cytoskeletal microtubules that forms during cellularization appears to be a key component in localizing the ftz mRNA. We conclude that RNA degradation and cellular structure normally work together to localize ftz RNA to its sites of synthesis.</jats:p>",
  "DOI": "10.1101/gad.1.10.1226",
  "type": "journal-article",
  "created": {
    "date-parts": [
      [
        2007,
        6,
        5
      ]
    ],
    "date-time": "2007-06-05T21:15:46Z",
    "timestamp": 1181078146000
  },
  "page": "1226-1237",
  "source": "Crossref",
  "is-referenced-by-count": 84,
  "title": "Cytoarchitecture and the patterning of fushi tarazu expression in the Drosophila blastoderm.",
  "prefix": "10.1101",
  "volume": "1",
  "author": [
    {
      "given": "B A",
      "family": "Edgar",
      "sequence": "first",
      "affiliation": []
    },
    {
      "given": "G M",
      "family": "Odell",
      "sequence": "additional",
      "affiliation": []
    },
    {
      "given": "G",
      "family": "Schubiger",
      "sequence": "additional",
      "affiliation": []
    }
  ],
  "member": "246",
  "published-online": {
    "date-parts": [
      [
        1987,
        12,
        1
      ]
    ]
  },
  "reference": [
    {
      "key": "2021111319444674000_1.10.1226.1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1083/jcb.100.4.1209"
    },
    {
      "key": "2021111319444674000_1.10.1226.2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0092-8674(85)90011-X"
    },
    {
      "key": "2021111319444674000_1.10.1226.3",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0092-8674(86)90543-X"
    },
    {
      "key": "2021111319444674000_1.10.1226.4",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0092-8674(81)90276-2"
    },
    {
      "issue": "Suppl.",
      "key": "2021111319444674000_1.10.1226.5",
      "first-page": "197",
      "article-title": "The stability and movement of mRNA in Xenopus oocytes and embryos.",
      "volume": "97",
      "year": "1986",
      "journal-title": "J. Emhiyol Exp. Morphol."
    },
    {
      "key": "2021111319444674000_1.10.1226.6",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0092-8674(85)90012-1"
    },
    {
      "key": "2021111319444674000_1.10.1226.7",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0092-8674(86)90009-7"
    },
    {
      "key": "2021111319444674000_1.10.1226.8",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0092-8674(86)90771-3"
    },
    {
      "key": "2021111319444674000_1.10.1226.9",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0092-8674(86)90517-9"
    },
    {
      "key": "2021111319444674000_1.10.1226.10",
      "unstructured": "Ennis, H.L. 1985. Developmental biology of the cellular slime mold. Roche Inst. Mol. Biol. Annu. Rep. 1985 28 \u00e2\u0080\u009331."
    },
    {
      "key": "2021111319444674000_1.10.1226.11",
      "doi-asserted-by": "publisher",
      "DOI": "10.1038/313284a0"
    },
    {
      "key": "2021111319444674000_1.10.1226.12",
      "doi-asserted-by": "crossref",
      "first-page": "31",
      "DOI": "10.1242/jcs.61.1.31",
      "article-title": "Studies of nuclear and cytoplasmic behavior during the five mitotic cycles that precede gastrulation in Drosophila embryogenesis.",
      "volume": "61",
      "year": "1983",
      "journal-title": "J. Cell Sci."
    },
    {
      "key": "2021111319444674000_1.10.1226.13",
      "doi-asserted-by": "crossref",
      "first-page": "155",
      "DOI": "10.1242/jcs.82.1.155",
      "article-title": "Nuclear antigens follow different pathways into daughter nuclei during mitosis in early Drosophila embryos.",
      "volume": "82",
      "year": "1986",
      "journal-title": "J. Cell Sci."
    },
    {
      "key": "2021111319444674000_1.10.1226.14",
      "doi-asserted-by": "publisher",
      "DOI": "10.1038/324120a0"
    },
    {
      "key": "2021111319444674000_1.10.1226.15",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0012-1606(71)90141-2"
    },
    {
      "key": "2021111319444674000_1.10.1226.16",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0092-8674(84)90418-5"
    },
    {
      "key": "2021111319444674000_1.10.1226.17",
      "doi-asserted-by": "publisher",
      "DOI": "10.1126/science.3755551"
    },
    {
      "key": "2021111319444674000_1.10.1226.18",
      "doi-asserted-by": "publisher",
      "DOI": "10.1083/jcb.86.1.212"
    },
    {
      "key": "2021111319444674000_1.10.1226.19",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0092-8674(86)90018-8"
    },
    {
      "key": "2021111319444674000_1.10.1226.20",
      "doi-asserted-by": "publisher",
      "DOI": "10.1038/318439a0"
    },
    {
      "key": "2021111319444674000_1.10.1226.21",
      "doi-asserted-by": "crossref",
      "first-page": "1659",
      "DOI": "10.1002/j.1460-2075.1986.tb04409.x",
      "article-title": "Correlative changes in homoeotic and segmentation gene expression in Kr\u00c3\u00bcppel mutant embryos of Drosophila.",
      "volume": "5",
      "year": "1986",
      "journal-title": "EMBO J."
    },
    {
      "key": "2021111319444674000_1.10.1226.22",
      "doi-asserted-by": "publisher",
      "DOI": "10.1038/324668a0"
    },
    {
      "key": "2021111319444674000_1.10.1226.23",
      "doi-asserted-by": "publisher",
      "DOI": "10.1083/jcb.102.4.1494"
    },
    {
      "key": "2021111319444674000_1.10.1226.24",
      "doi-asserted-by": "publisher",
      "DOI": "10.1038/321493a0"
    },
    {
      "key": "2021111319444674000_1.10.1226.25",
      "doi-asserted-by": "publisher",
      "DOI": "10.1038/317040a0"
    },
    {
      "key": "2021111319444674000_1.10.1226.26",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0092-8674(85)90307-1"
    },
    {
      "key": "2021111319444674000_1.10.1226.27",
      "doi-asserted-by": "publisher",
      "DOI": "10.1038/310025a0"
    },
    {
      "key": "2021111319444674000_1.10.1226.28",
      "doi-asserted-by": "publisher",
      "DOI": "10.1083/jcb.102.6.2015"
    },
    {
      "key": "2021111319444674000_1.10.1226.29",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0092-8674(86)90515-5"
    },
    {
      "key": "2021111319444674000_1.10.1226.30",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0012-1606(75)90027-5"
    },
    {
      "key": "2021111319444674000_1.10.1226.31",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0012-1606(76)90278-5"
    },
    {
      "key": "2021111319444674000_1.10.1226.32",
      "doi-asserted-by": "crossref",
      "first-page": "565",
      "DOI": "10.1093/icb/17.3.565",
      "article-title": "Determination during early embryogenesis of Drosophila melanogaster.",
      "volume": "17",
      "year": "1977",
      "journal-title": "Am. Zool."
    },
    {
      "key": "2021111319444674000_1.10.1226.33",
      "doi-asserted-by": "publisher",
      "DOI": "10.1146/annurev.cb.02.110186.000405"
    },
    {
      "key": "2021111319444674000_1.10.1226.34",
      "first-page": "179",
      "article-title": "Dynamics of the nuclear envelope and of nuclear pore complexes during mitosis in the Drosophila embryo.",
      "volume": "34",
      "year": "1984",
      "journal-title": "Eur. J. Cell Biol."
    },
    {
      "key": "2021111319444674000_1.10.1226.35",
      "doi-asserted-by": "publisher",
      "DOI": "10.1038/327383a0"
    },
    {
      "key": "2021111319444674000_1.10.1226.36",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0012-1606(76)90070-1"
    },
    {
      "key": "2021111319444674000_1.10.1226.37",
      "unstructured": "Walter, M. and B.M. Alberts. 1984. Intermediate filaments in tissue culture cells and early embryos of Drosophila melanogaster. In Molecular biology of development (ed. R. Firtel and E. Davidson), pp. 263\u00e2\u0080\u0093272. UCLA Symp. Mol. Cell. Biol. New Series. Alan R. Liss, New York."
    },
    {
      "key": "2021111319444674000_1.10.1226.38",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0014-4827(83)90113-1"
    },
    {
      "key": "2021111319444674000_1.10.1226.39",
      "first-page": "201",
      "article-title": "Microtubule arrays present during the syncytial and cellular blastoderm stage of the early Drosophila embryo.",
      "volume": "163",
      "year": "1985",
      "journal-title": "Exp. Cell Res."
    },
    {
      "key": "2021111319444674000_1.10.1226.40",
      "doi-asserted-by": "publisher",
      "DOI": "10.1038/318433a0"
    },
    {
      "key": "2021111319444674000_1.10.1226.41",
      "unstructured": "Wieschaus, E. and C. N\u00c3\u00bcsslein-Volhard. 1986. Looking at embryos. In Drosophila: A practical approach (ed. D.B. Roberts), pp. 221\u00e2\u0080\u0093223. IRL Press, Oxford."
    },
    {
      "key": "2021111319444674000_1.10.1226.42",
      "doi-asserted-by": "publisher",
      "DOI": "10.1083/jcb.82.1.114"
    },
    {
      "key": "2021111319444674000_1.10.1226.43",
      "first-page": "97",
      "article-title": "Division and migration of nuclei during early embryogenesis of Drosophila melanogaster.",
      "volume": "25",
      "year": "1976",
      "journal-title": "J. Microsc. Biol. Cell"
    }
  ],
  "container-title": "Genes &amp; Development",
  "original-title": [],
  "language": "en",
  "link": [
    {
      "URL": "https://syndication.highwire.org/content/doi/10.1101/gad.1.10.1226",
      "content-type": "unspecified",
      "content-version": "vor",
      "intended-application": "similarity-checking"
    }
  ],
  "deposited": {
    "date-parts": [
      [
        2021,
        11,
        14
      ]
    ],
    "date-time": "2021-11-14T03:50:38Z",
    "timestamp": 1636861838000
  },
  "score": 1,
  "resource": {
    "primary": {
      "URL": "http://genesdev.cshlp.org/lookup/doi/10.1101/gad.1.10.1226"
    }
  },
  "subtitle": [],
  "short-title": [],
  "issued": {
    "date-parts": [
      [
        1987,
        12
      ]
    ]
  },
  "references-count": 43,
  "journal-issue": {
    "issue": "10",
    "published-print": {
      "date-parts": [
        [
          1987,
          12
        ]
      ]
    }
  },
  "alternative-id": [
    "10.1101/gad.1.10.1226"
  ],
  "URL": "http://dx.doi.org/10.1101/gad.1.10.1226",
  "relation": {},
  "ISSN": [
    "0890-9369",
    "1549-5477"
  ],
  "subject": [],
  "container-title-short": "Genes Dev.",
  "published": {
    "date-parts": [
      [
        1987,
        12
      ]
    ]
  }
}