DOI: 10.1099/vir.0.80598-0. RNA interference of rotavirus segment 11 mRNA reveals the essential role of NSP5 in the virus replicative cycle

RNA interference of rotavirus segment 11 mRNA reveals the essential role of NSP5 in the virus replicative cycle

10.1099/vir.0.80598-0

Crossref journal-article

Microbiology Society

Journal of General Virology (345)

Abstract

Rotavirus genomes contain 11 double-stranded (ds) RNA segments. Genome segment 11 encodes the non-structural protein NSP5 and, in some strains, also NSP6. NSP5 is produced soon after viral infection and localizes in cytoplasmic viroplasms, where virus replication takes place. RNA interference by small interfering (si) RNAs targeted to genome segment 11 mRNA of two different strains blocked production of NSP5 in a strain-specific manner, with a strong effect on the overall replicative cycle: inhibition of viroplasm formation, decreased production of other structural and non-structural proteins, synthesis of viral genomic dsRNA and production of infectious particles. These effects were shown not to be due to inhibition of NSP6. The results obtained strengthen the importance of secondary transcription/translation in rotavirus replication and demonstrate that NSP5 is essential for the assembly of viroplasms and virus replication.

Bibliography

Campagna, M., Eichwald, C., Vascotto, F., & Burrone, O. R. (2005). RNA interference of rotavirus segment 11 mRNA reveals the essential role of NSP5 in the virus replicative cycle. Journal of General Virology, 86(5), 1481â1487.

Authors 4

Michela Campagna (first)
Catherine Eichwald (additional)
Fulvia Vascotto (additional)
Oscar R. Burrone (additional)

References 38 Referenced 87

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Dates

Type	When
Created	20 years, 4 months ago (April 14, 2005, 6:52 p.m.)
Deposited	4 years, 10 months ago (Oct. 22, 2020, 12:18 p.m.)
Indexed	1 month, 3 weeks ago (July 8, 2025, 1:11 a.m.)
Issued	20 years, 4 months ago (May 1, 2005)
Published	20 years, 4 months ago (May 1, 2005)
Published Print	20 years, 4 months ago (May 1, 2005)

Funders 0

None

BibTeX

@article{Campagna_2005, title={RNA interference of rotavirus segment 11 mRNA reveals the essential role of NSP5 in the virus replicative cycle}, volume={86}, ISSN={1465-2099}, url={http://dx.doi.org/10.1099/vir.0.80598-0}, DOI={10.1099/vir.0.80598-0}, number={5}, journal={Journal of General Virology}, publisher={Microbiology Society}, author={Campagna, Michela and Eichwald, Catherine and Vascotto, Fulvia and Burrone, Oscar R.}, year={2005}, month=may, pages={1481–1487} }

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