DOI: 10.1099/vir.0.80007-0. Complete nucleotide sequence and genome organization of Grapevine leafroll-associated virus 3, type member of the genus Ampelovirus

Complete nucleotide sequence and genome organization of Grapevine leafroll-associated virus 3, type member of the genus Ampelovirus

10.1099/vir.0.80007-0

Crossref journal-article

Microbiology Society

Journal of General Virology (345)

Abstract

This study reports on the complete genome sequence of Grapevine leafroll-associated virus 3, the type member of the genus Ampelovirus. The genome is 17 919 nt in size and contains 13 open reading frames (ORFs). Previously, the sequence of 13 154 nt of the 3′-terminal of the genome was reported. The newly sequenced portion contains a 158 nt 5′ UTR, a single papain-like protease and a methyltransferase-like (MT) domain. ORF1a encodes a large polypeptide with a molecular mass of 245 kDa. With a predicted +1 frameshift, the large fusion protein generated from ORF1a/1b would produce a 306 kDa polypeptide. Phylogenetic analysis using MT domains further supports the creation of the genus Ampelovirus for mealy-bug-transmitted viruses in the family Closteroviridae.

Bibliography

Ling, K.-S., Zhu, H.-Y., & Gonsalves, D. (2004). Complete nucleotide sequence and genome organization of Grapevine leafroll-associated virus 3, type member of the genus Ampelovirus. Journal of General Virology, 85(7), 2099â2102.

Authors 3

Kai-Shu Ling (first)
Hai-Ying Zhu (additional)
Dennis Gonsalves (additional)

References 15 Referenced 56

10.1006/viro.1994.1034 / Virology / Beet yellows closterovirus: complete genome structure and identification of a leader papain-like thiol protease by Agranovsky (1994)
10.1146/annurev.py.32.090194.001401 / Annu Rev Phytopathol / Molecular biology and evolution of closteroviruses: sophisticated build-up of large RNA genomes by Dolja (1994)
10.1094/PHYTO.2001.91.3.268 / Phytopathology / Partial characterization of a closterovirus associated with apple mealybug-transmitted little cherry disease in North America by Eastwell (2001)
10.1111/j.1439-0434.1990.tb04247.x / J Phytopathol / Characterization of closterovirus-like particles associated with grapevine leafroll disease by Hu (1990)
10.1099/0022-1317-78-8-2067 / J Gen Virol / Complete genome structure and phylogenetic analysis of little cherry virus, a mealybug transmissible closterovirus by Jelkmann (1997)
10.1146/annurev.phyto.38.1.293 / Annu Rev Phytopathol / Genetic diversity and evolution of closteroviruses by Karasev (2000)
10.1006/viro.1995.1182 / Virology / Complete sequence of the citrus tristeza virus RNA genome by Karasev (1995)
10.1006/viro.1995.1133 / Virology / Genome structure and phylogenetic analysis of lettuce infectious yellows virus, a whitefly-transmitted, bipartite closterovirus by Klaassen (1995)
10.1007/s007050050145 / Arch Virol / The coat protein gene of grapevine leafroll associated closterovirus-3: cloning, nucleotide sequencing and expression in transgenic plants by Ling (1997)
10.1099/0022-1317-79-5-1299 / J Gen Virol / Nucleotide sequence of the 3′-terminal two-thirds of the grapevine leafroll-associated virus-3 genome reveals a typical monopartite closterovirus by Ling (1998)
10.1007/s007050200048 / Arch Virol / The family Closteroviridae revised by Martelli (2002)
10.1099/0022-1317-82-1-1 / J Gen Virol / Nucleotide sequence, genome organization and phylogenetic analysis of pineapple mealybug wilt-associated virus-2 by Melzer (2001)
10.1094/PHYTO.2001.91.3.261 / Phytopathology / Detection and partial characterization of a second closterovirus associated with little cherry disease, little cherry virus-2 by Rott (2001)
{'key': 'R14', 'volume-title': 'Molecular Cloning: a Laboratory Manual', 'author': 'Sambrook', 'year': '1989'} / Molecular Cloning: a Laboratory Manual by Sambrook (1989)
10.1099/0022-1317-79-5-1289 / J Gen Virol / Nucleotide sequence and genome organization of grapevine leafroll-associated virus-2 is similar to beet yellows virus, the closterovirus type member by Zhu (1998)

Dates

Type	When
Created	21 years, 2 months ago (June 24, 2004, 7:52 p.m.)
Deposited	4 years, 10 months ago (Oct. 6, 2020, 10:39 a.m.)
Indexed	4 weeks, 1 day ago (Aug. 6, 2025, 9:34 a.m.)
Issued	21 years, 2 months ago (July 1, 2004)
Published	21 years, 2 months ago (July 1, 2004)
Published Print	21 years, 2 months ago (July 1, 2004)

Funders 0

None

BibTeX

@article{Ling_2004, title={Complete nucleotide sequence and genome organization of Grapevine leafroll-associated virus 3, type member of the genus Ampelovirus}, volume={85}, ISSN={1465-2099}, url={http://dx.doi.org/10.1099/vir.0.80007-0}, DOI={10.1099/vir.0.80007-0}, number={7}, journal={Journal of General Virology}, publisher={Microbiology Society}, author={Ling, Kai-Shu and Zhu, Hai-Ying and Gonsalves, Dennis}, year={2004}, month=jul, pages={2099–2102} }

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