Functional interactions between type IV secretion systems involved in DNA transfer and virulence
10.1099/mic.0.28410-0
Crossref journal-article
Microbiology Society
Microbiology (345)
Abstract

This paper reports an analysis of the functional interactions between type IV secretion systems (T4SS) that are part of the conjugative machinery for horizontal DNA transfer (cT4SS), and T4SS involved in bacterial pathogenicity (pT4SS). The authors' previous work showed that a conjugative coupling protein (T4CP) interacts with the VirB10-type component of the T4SS in order to recruit the protein–DNA complex to the transporter for conjugative DNA transfer. This study now shows by two-hybrid analysis that conjugative T4CPs also interact with the VirB10 element of the pT4SS of Agrobacterium tumefaciens (At), Bartonella tribocorum (Bt) and Brucella suis (Bs). Moreover, the VirB10 component of a cT4SS (protein TrwE of plasmid R388) could be partially substituted by that of a pT4SS (protein TrwE of Bt) for conjugation. This result opens the way for the construction of hybrid T4SS that deliver DNA into animal cells. Interestingly, in the presence of part of the Bs T4SS the R388 T4SS protein levels were decreased and R388 conjugation was strongly inhibited. Complementation assays between the Trw systems of R388 and Bt showed that only individual components from the so-called ‘core complex’ could be exchanged, supporting the concept that this core is the common scaffold for the transport apparatus while the other ‘peripheral components' are largely system-specific.

Bibliography

de Paz, H. D., Sangari, F. J., Bolland, S., García-Lobo, J. M., Dehio, C., de la Cruz, F., & Llosa, M. (2005). Functional interactions between type IV secretion systems involved in DNA transfer and virulence. Microbiology, 151(11), 3505–3516.

Authors 7
  1. Héctor D. de Paz (first)
  2. Félix J. Sangari (additional)
  3. Silvia Bolland (additional)
  4. Juan M. García-Lobo (additional)
  5. Christoph Dehio (additional)
  6. Fernando de la Cruz (additional)
  7. Matxalen Llosa (additional)
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Dates
Type When
Created 19 years, 10 months ago (Nov. 4, 2005, 1:28 p.m.)
Deposited 4 years, 9 months ago (Nov. 6, 2020, 2:30 p.m.)
Indexed 1 year, 4 months ago (April 25, 2024, 7:49 a.m.)
Issued 19 years, 10 months ago (Nov. 1, 2005)
Published 19 years, 10 months ago (Nov. 1, 2005)
Published Print 19 years, 10 months ago (Nov. 1, 2005)
Funders 0

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@article{de_Paz_2005, title={Functional interactions between type IV secretion systems involved in DNA transfer and virulence}, volume={151}, ISSN={1465-2080}, url={http://dx.doi.org/10.1099/mic.0.28410-0}, DOI={10.1099/mic.0.28410-0}, number={11}, journal={Microbiology}, publisher={Microbiology Society}, author={de Paz, Héctor D. and Sangari, Félix J. and Bolland, Silvia and García-Lobo, Juan M. and Dehio, Christoph and de la Cruz, Fernando and Llosa, Matxalen}, year={2005}, month=nov, pages={3505–3516} }