DOI: 10.1099/mic.0.28277-0. Factors triggering type III secretion in Pseudomonas aeruginosa

Factors triggering type III secretion in Pseudomonas aeruginosa

10.1099/mic.0.28277-0

Crossref journal-article

Microbiology Society

Microbiology (345)

Abstract

The type III secretion system ofPseudomonas aeruginosais tightly regulated by various environmental signals, such as low calcium and contact with the host cell. However, the exact signals triggering type III secretion are unknown. The present study describes the finding that secretion ofP. aeruginosatype III effector molecules requires protein factors from serum and L broth, designated type III secretion factors (TSFs), in addition to the low-calcium environment. In the absence of TSF or calcium chelator EGTA, basal levels of type III effector molecules are accumulated intracellularly. Addition of TSF and EGTA together effectively triggers the secretion of pre-existing effector molecules in a short time, even before the active expression of type III genes; thus, active type III gene expression does not seem to be a prerequisite for type III secretion. A search for TSF molecules in serum and L broth resulted in the identification of albumin and casein as the functional TSF molecules. Although there is no clear sequence similarity between albumin and casein, both proteins are known to have a low-affinity, high-capacity calcium-binding property. Tests of well-studied calcium-binding proteins seemed to indicate that low-affinity calcium-binding proteins have TSF activity, although the requirement of low-affinity calcium-binding ability for the TSF activity is not clear.P. aeruginosaseems to have evolved a sensing mechanism to detect target cells for type III injection through host-derived proteins in combination with a low-calcium signal. Disruption of the bacterial ability to sense low calcium or TSF might be a valid avenue to the effective control of this bacterial pathogen.

Bibliography

Kim, J., Ahn, K., Min, S., Jia, J., Ha, U., Wu, D., & Jin, S. (2005). Factors triggering type III secretion in Pseudomonas aeruginosa. Microbiology, 151(11), 3575â3587.

Authors 7

Jaewha Kim (first)
Kyungseop Ahn (additional)
Sungran Min (additional)
Jinghua Jia (additional)
Unhwan Ha (additional)
Donghai Wu (additional)
Shouguang Jin (additional)

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Dates

Type	When
Created	19 years, 9 months ago (Nov. 4, 2005, 1:28 p.m.)
Deposited	4 years, 1 month ago (July 19, 2021, 2:19 a.m.)
Indexed	2 months, 3 weeks ago (May 27, 2025, 2:03 a.m.)
Issued	19 years, 9 months ago (Nov. 1, 2005)
Published	19 years, 9 months ago (Nov. 1, 2005)
Published Print	19 years, 9 months ago (Nov. 1, 2005)

Funders 0

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BibTeX

@article{Kim_2005, title={Factors triggering type III secretion in Pseudomonas aeruginosa}, volume={151}, ISSN={1465-2080}, url={http://dx.doi.org/10.1099/mic.0.28277-0}, DOI={10.1099/mic.0.28277-0}, number={11}, journal={Microbiology}, publisher={Microbiology Society}, author={Kim, Jaewha and Ahn, Kyungseop and Min, Sungran and Jia, Jinghua and Ha, Unhwan and Wu, Donghai and Jin, Shouguang}, year={2005}, month=nov, pages={3575–3587} }

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