DOI: 10.1099/mic.0.27557-0. Pleiotropic effect of the SCO2127 gene on the glucose uptake, glucose kinase activity and carbon catabolite repression in Streptomyces peucetius var. caesius

Pleiotropic effect of the SCO2127 gene on the glucose uptake, glucose kinase activity and carbon catabolite repression in Streptomyces peucetius var. caesius

10.1099/mic.0.27557-0

Crossref journal-article

Microbiology Society

Microbiology (345)

Abstract

SCO2127 and SCO2126 (glkA) are adjacent regions located in Streptomyces coelicolor DNA. glkA encodes glucose kinase (Glk), which has been implicated in carbon catabolite repression (CCR) in the genus Streptomyces. In this work, the glkA and SCO2127 genes from S. coelicolor were used, either individually or together, to transform three mutants of Streptomyces peucetius var. caesius resistant to CCR. These mutants present decreased levels of Glk, and deficiency in glucose transport. When the mutants were transformed with a plasmid containing the SCO2127 sequence, glucose uptake and Glk activity values were increased to levels similar to or higher than those of the original strain, and each strain regained sensitivity to CCR. This result was surprising considering that the putative SCO2127 amino acid sequence does not seem to encode a glucose permease or a Glk. In agreement with these results, an increase in glkA mRNA levels was observed in a CCR-resistant mutant transformed with SCO2127 compared with those of the original strain and the CCR-resistant mutant itself. As expected, recombinants containing the glkA sequence reverted Glk to normal activity values, but glucose uptake remained deficient. The data suggest that the SCO2127 gene product enhances transcription of both genes, and support the first specific role for this region in Streptomyces species. The physiological consequence of this effect is an increase in the glucose catabolites that may be involved in eliciting CCR in this genus.

Bibliography

GuzmÃ¡n, S., Carmona, A., Escalante, L., Imriskova, I., LÃ³pez, R., RodrÃguez-Sanoja, R., Ruiz, B., ServÃn-GonzÃ¡lez, L., SÃ¡nchez, S., & Langley, E. (2005). Pleiotropic effect of the SCO2127 gene on the glucose uptake, glucose kinase activity and carbon catabolite repression in Streptomyces peucetius var. caesius. Microbiology, 151(5), 1717â1723.

Authors 10

Silvia Guzmán (first)
Alonso Carmona (additional)
Laura Escalante (additional)
Iveta Imriskova (additional)
Ruth López (additional)
Romina Rodríguez-Sanoja (additional)
Beatriz Ruiz (additional)
Luis Servín-González (additional)
Sergio Sánchez (additional)
Elizabeth Langley (additional)

References 28 Referenced 17

10.1111/j.1365-2958.1992.tb01463.x / Mol Microbiol / The glucose kinase gene of Streptomyces coelicolor A3(2): its nucleotide sequence, transcriptional analysis and role in glucose repression by Angell (1992)
10.1007/BF00283514 / Mol Gen Genet / Glucose repression in Streptomyces coelicolor A3(2): a likely regulatory role for glucose kinase by Angell (1994)
10.1002/bit.260110607 / Biotechnol Bioeng / Adriamycin, 14-hydroxydaunomycin, a new antitumor antibiotic from Streptomyces peucetius var. caesius by Arcamone (1969)
{'key': 'R4', 'first-page': '137', 'article-title': 'Protoplast fusion in Streptomyces: conditions for efficient genetic recombination and cell regeneration', 'volume': '127', 'author': 'Baltz', 'year': '1981', 'journal-title': 'J Gen Microbiol'} / J Gen Microbiol / Protoplast fusion in Streptomyces: conditions for efficient genetic recombination and cell regeneration by Baltz (1981)
10.1128/JB.186.5.1362-1373.2004 / J Bacteriol / In silico and transcriptional analysis of carbohydrate uptake systems of Streptomyces coelicolor A3(2) by Bertram (2004)
10.1016/S0378-1097(02)00559-1 / FEMS Microbiol Lett / Carbon catabolite repression in bacteria: choice of the carbon source and autoregulatory limitation of sugar utilization by Brückner (2002)
10.1128/JB.170.3.1168-1174.1988 / J Bacteriol / New loci required for Streptomyces coelicolor morphological and physiological differentiation by Champness (1988)
10.1016/0003-2697(87)90021-2 / Anal Biochem / Single-step method of RNA isolation by acid guanidinium thiocyanate–phenol–chloroform extraction by Chomczynski (1987)
{'key': 'R9', 'first-page': '127', 'article-title': 'Carbon source regulation of idiolite biosynthesis in actinomycetes', 'volume-title': 'Regulation of Secondary Metabolism in Actinomycetes', 'author': 'Demain', 'year': '1989'} / Regulation of Secondary Metabolism in Actinomycetes / Carbon source regulation of idiolite biosynthesis in actinomycetes by Demain (1989)
10.1007/s002530051562 / Appl Microbiol Biotechnol / Glucose repression of anthracycline formation in Streptomyces peucetius var. caesius by Escalante (1999)
10.1007/BF00138547 / Biotechnol Lett / Glucose and glycerol repression of amylase in Streptomyces kanamyceticus and isolation of deregulated mutants by Flores (1993)
{'key': 'R12', 'first-page': '2417', 'article-title': 'Glucose repression of carbon uptake and metabolism in Streptomyces coelicolor A3(2) and its perturbation in mutants resistant to 2-deoxyglucose', 'volume': '128', 'author': 'Hodgson', 'year': '1982', 'journal-title': 'J Gen Microbiol'} / J Gen Microbiol / Glucose repression of carbon uptake and metabolism in Streptomyces coelicolor A3(2) and its perturbation in mutants resistant to 2-deoxyglucose by Hodgson (1982)
10.1007/BF00436199 / Mol Gen Genet / Genetic mapping, cloning and physiological aspects of the glucose kinase gene of Streptomyces coelicolor by Ikeda (1984)
10.1006/abbi.2001.2514 / Arch Biochem Biophys / Purification and characterization of glucose kinase from Streptomyces peucetius var. caesius by Imriskova (2001)
10.1128/JB.177.12.3587-3588.1995 / J Bacteriol / The glucose kinase gene of Streptomyces coelicolor is not required for glucose repression of the chi63 promoter by Ingram (1995)
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Dates

Type	When
Created	20 years, 3 months ago (May 3, 2005, 5:28 p.m.)
Deposited	4 years, 9 months ago (Nov. 6, 2020, 1:37 p.m.)
Indexed	1 year, 10 months ago (Oct. 2, 2023, 6:59 a.m.)
Issued	20 years, 3 months ago (May 1, 2005)
Published	20 years, 3 months ago (May 1, 2005)
Published Print	20 years, 3 months ago (May 1, 2005)

Funders 0

None

BibTeX

@article{Guzm_n_2005, title={Pleiotropic effect of the SCO2127 gene on the glucose uptake, glucose kinase activity and carbon catabolite repression in Streptomyces peucetius var. caesius}, volume={151}, ISSN={1465-2080}, url={http://dx.doi.org/10.1099/mic.0.27557-0}, DOI={10.1099/mic.0.27557-0}, number={5}, journal={Microbiology}, publisher={Microbiology Society}, author={Guzmán, Silvia and Carmona, Alonso and Escalante, Laura and Imriskova, Iveta and López, Ruth and Rodríguez-Sanoja, Romina and Ruiz, Beatriz and Servín-González, Luis and Sánchez, Sergio and Langley, Elizabeth}, year={2005}, month=may, pages={1717–1723} }

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