DOI: 10.1099/mic.0.27058-0. An aspartic proteinase gene family in the filamentous fungus Botrytis cinerea contains members with novel features

An aspartic proteinase gene family in the filamentous fungus Botrytis cinerea contains members with novel features

10.1099/mic.0.27058-0

Crossref journal-article

Microbiology Society

Microbiology (345)

Abstract

Botrytis cinerea, an important fungal plant pathogen, secretes aspartic proteinase (AP) activity in axenic cultures. No cysteine, serine or metalloproteinase activity could be detected. Proteinase activity was higher in culture medium containing BSA or wheat germ extract, as compared to minimal medium. A proportion of the enzyme activity remained in the extracellular glucan sheath. AP was also the only type of proteinase activity in fluid obtained from B. cinerea-infected tissue of apple, pepper, tomato and zucchini. Five B. cinerea genes encoding an AP were cloned and denoted Bcap1–5. Features of the encoded proteins are discussed. BcAP1, especially, has novel characteristics. A phylogenetic analysis was performed comprising sequences originating from different kingdoms. BcAP1 and BcAP5 did not cluster in a bootstrap-supported clade. BcAP2 clusters with vacuolar APs. BcAP3 and BcAP4 cluster with secreted APs in a clade that also contains glycosylphosphatidylinositol-anchored proteinases from Saccharomyces cerevisiae and Candida albicans. All five Bcap genes are expressed in liquid cultures. Transcript levels of Bcap1, Bcap2, Bcap3 and Bcap4 are subject to glucose and peptone repression. Transcripts from all five Bcap genes were detected in infected plant tissue, indicating that at least part of the AP activity in planta originates from the pathogen.

Bibliography

ten Have, A., Dekkers, E., Kay, J., Phylip, L. H., & van Kan, J. A. L. (2004). An aspartic proteinase gene family in the filamentous fungus Botrytis cinerea contains members with novel features. Microbiology, 150(7), 2475â2489.

Authors 5

Arjen ten Have (first)
Ester Dekkers (additional)
John Kay (additional)
Lowri H. Phylip (additional)
Jan A. L. van Kan (additional)

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Dates

Type	When
Created	21 years, 1 month ago (July 15, 2004, 1:45 p.m.)
Deposited	4 years, 9 months ago (Nov. 6, 2020, 12:27 p.m.)
Indexed	1 month, 3 weeks ago (July 2, 2025, 10:39 a.m.)
Issued	21 years, 1 month ago (July 1, 2004)
Published	21 years, 1 month ago (July 1, 2004)
Published Print	21 years, 1 month ago (July 1, 2004)

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BibTeX

@article{ten_Have_2004, title={An aspartic proteinase gene family in the filamentous fungus Botrytis cinerea contains members with novel features}, volume={150}, ISSN={1465-2080}, url={http://dx.doi.org/10.1099/mic.0.27058-0}, DOI={10.1099/mic.0.27058-0}, number={7}, journal={Microbiology}, publisher={Microbiology Society}, author={ten Have, Arjen and Dekkers, Ester and Kay, John and Phylip, Lowri H. and van Kan, Jan A. L.}, year={2004}, month=jul, pages={2475–2489} }

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  "abstract": "<jats:p>\n                  <jats:italic>Botrytis cinerea</jats:italic>, an important fungal plant pathogen, secretes aspartic proteinase (AP) activity in axenic cultures. No cysteine, serine or metalloproteinase activity could be detected. Proteinase activity was higher in culture medium containing BSA or wheat germ extract, as compared to minimal medium. A proportion of the enzyme activity remained in the extracellular glucan sheath. AP was also the only type of proteinase activity in fluid obtained from <jats:italic>B. cinerea</jats:italic>-infected tissue of apple, pepper, tomato and zucchini. Five <jats:italic>B. cinerea</jats:italic> genes encoding an AP were cloned and denoted <jats:italic>Bcap1\u20135</jats:italic>. Features of the encoded proteins are discussed. BcAP1, especially, has novel characteristics. A phylogenetic analysis was performed comprising sequences originating from different kingdoms. BcAP1 and BcAP5 did not cluster in a bootstrap-supported clade. BcAP2 clusters with vacuolar APs. BcAP3 and BcAP4 cluster with secreted APs in a clade that also contains glycosylphosphatidylinositol-anchored proteinases from <jats:italic>Saccharomyces cerevisiae</jats:italic> and <jats:italic>Candida albicans</jats:italic>. All five <jats:italic>Bcap</jats:italic> genes are expressed in liquid cultures. Transcript levels of <jats:italic>Bcap1</jats:italic>, <jats:italic>Bcap2</jats:italic>, <jats:italic>Bcap3</jats:italic> and <jats:italic>Bcap4</jats:italic> are subject to glucose and peptone repression. Transcripts from all five <jats:italic>Bcap</jats:italic> genes were detected in infected plant tissue, indicating that at least part of the AP activity <jats:italic>in planta</jats:italic> originates from the pathogen.</jats:p>",
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