DOI: 10.1099/13500872-142-3-667. The rRNA operons of Mycobacterium smegmatis and Mycobacterium tuberculosis : comparison of promoter elements and of neighbouring upstream genes

The rRNA operons of Mycobacterium smegmatis and Mycobacterium tuberculosis : comparison of promoter elements and of neighbouring upstream genes

10.1099/13500872-142-3-667

Crossref journal-article

Microbiology Society

Microbiology (345)

Abstract

Mycobacterium smegmatis has two rRNA (rrn) operons designated rrnA f and rrnB f. Appropriate restriction fragments of genomic DNA containing sequences immediately upstream from the 16S rRNA genes were cloned. We now report the nucleotide sequence of 552 bp upstream from the 5′-end of the Box AL antitermination element of the leader region of the rrnA f operon. The 5′-end of this segment of DNA was found to comprise 113 codons of an ORF encoding a protein which is significantly similar to UDP-N-acetylglucosamine 1-carboxyvinyl-transferase (EC 2.5.1.7), which is important to cell wall synthesis. A homologous ORF is located immediately upstream from the single rrn (rrnA s) operons of Mycobacterium tuberculosis and Mycobacterium leprae. Primer-extension analysis of the RNA fraction of M. smegmatis revealed four products which were related to transcription start points; the rrnB f operon appears to have a single promoter whereas the rrnA f operon has three (P1, P2 and P3). Analysis of M. tuberculosis RNA revealed two products corresponding to transcripts directed by promoters homologous with P1 and P3 of the rrnA f of M. smegmatis. Thus, the promoter and upstream regions of the rrnA f operon of M. smegmatis and the rrnA s operon of M. tuberculosis are homologous. The presence of P2 in M. smegmatis and its absence from M. tuberculosis is attributable to insertions/deletions of 97 bp.

Bibliography

Gonzalez-y-Merchand, J. A., Colston, M. J., & Cox, R. A. (1996). The rRNA operons of Mycobacterium smegmatis and Mycobacterium tuberculosisâ¯: comparison of promoter elements and of neighbouring upstream genes. Microbiology, 142(3), 667â674.

Authors 3

Jorge A. Gonzalez-y-Merchand (first)
M. Joseph Colston (additional)
Robert A. Cox (additional)

References 27 Referenced 67

10.1016/S0022-2836(05)80360-2 / J Mol Biol / Basic local alignment search tool by Altschul (1990)
10.1111/j.1365-2958.1988.tb00065.x / Mol Microbiol / Transcriptional analysis of the 16S rRNA gene of the rrtiD gene of Streptomyces coelicolor A3(2) by Baylis (1988)
10.1016/0006-291X(86)90452-3 / Biochem Biophys Res Commun / Mycobacteria possess a surprisingly small number of ribosomal RNA genes in relation to the size of their genome by Bercovier (1986)
{'key': 'R4', 'first-page': '1527', 'article-title': 'Modulation of chemical composition and other parameters of the cell growth rate', 'volume-title': 'In Escherichia coli and Salmonella typhimurium: Cellular and Molecular Biology', 'author': 'Bremer', 'year': '1987'} / In Escherichia coli and Salmonella typhimurium: Cellular and Molecular Biology / Modulation of chemical composition and other parameters of the cell growth rate by Bremer (1987)
10.1002/j.1460-2075.1992.tb05511.x / EMBOJ / Comparison of the seven ribosomal RNA operons in Escherichia coli by Condon (1992)
10.1111/j.1574-6968.1994.tb06755.x / FEMS Microbiol Lett / UDP-N-acetylglucosamine 1-carboxyvinyl-transferase from Acinetobacter calcoaceticus by Ehrt (1994)
10.1111/j.1574-6968.1996.tb07968.x / FEMS Microbiol Lett / A novel method for the isolation of my co-bacterial DNA by Gonzalez-y-Merchand (1986)
10.1016/0092-8674(86)90498-8 / Cell / DNA determinants of rRNA synthesis in E. coli, growth rate dependent regulation, feedback inhibition, upstream activation, anti-termination by Gourse (1986)
10.1099/13500872-140-1-123 / Microbiology / Nucleotide sequence and secondary structures of precursor 16S rRNA of slow-growing mycobacteria by Ji (1994a)
10.1099/13500872-140-7-1763 / Microbiology / Nucleotide sequences of the spacer-1, spacer-2 and trailer regions of the rrn operons and secondary structures of precursor 23S rRNA and precursor 5S rRNA of slow-growing mycobacteria by Ji (1994b)
10.1099/00221287-140-10-2829 / Microbiology / The ribosomal RNA (rrn) operons of fast-growing mycobacteria: primary and secondary structures and their relation to rrn operons of pathogenic slow-growers by Ji (1994c)
10.1099/00221287-138-8-1717 / J Gen Microbiol / The nucleotide sequence of the promoter, 16S rRNA and spacer region of the ribosomal RNA operon of Mycobacterium tuberculosis and comparison with Mycobacterium leprae precursor rRNA by Kempsell (1992)
10.1128/JB.168.1.72-80.1986 / J Bacteriol / Cloning and expression of Mycobacterium bovis BCG DNA in “Streptomyces lividans” by Kieser (1986)
10.1093/nar/18.18.5558 / Nucleic Acids Res / Nucleotide sequence of the 16S rRNA from Mycobacterium leprae by Liesack (1990)
10.1016/0014-5793(91)80372-A / FEBS Lett / Complete nucleotide sequence of the Mycobacterium leprae 23S and 5S rRNA genes plus flanking regions and their potential in designing diagnostic oligonucleotide probes by Liesack (1991)
10.1093/nar/11.18.6301 / Nucleic Acids Res / Structure and organization of rRNA operons in the region of the replication origin of the Bacillus subtilis chromosome. by Ogasawara (1983)
10.1111/j.1365-2958.1995.tb02249.x / Mol Microbiol / Characterization of RNA polymerase and two sigma-factor genes from Mycobacterium smegmatis by Predich (1995)
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Dates

Type	When
Created	16 years, 2 months ago (June 5, 2009, 12:53 a.m.)
Deposited	4 years, 6 months ago (March 3, 2021, 9:38 a.m.)
Indexed	5 days, 6 hours ago (Aug. 29, 2025, 5:51 a.m.)
Issued	29 years, 6 months ago (March 1, 1996)
Published	29 years, 6 months ago (March 1, 1996)
Published Print	29 years, 6 months ago (March 1, 1996)

Funders 0

None

BibTeX

@article{Gonzalez_y_Merchand_1996, title={The rRNA operons of Mycobacterium smegmatis and Mycobacterium tuberculosis : comparison of promoter elements and of neighbouring upstream genes}, volume={142}, ISSN={1465-2080}, url={http://dx.doi.org/10.1099/13500872-142-3-667}, DOI={10.1099/13500872-142-3-667}, number={3}, journal={Microbiology}, publisher={Microbiology Society}, author={Gonzalez-y-Merchand, Jorge A. and Colston, M. Joseph and Cox, Robert A.}, year={1996}, month=mar, pages={667–674} }

JSON

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}