DOI: 10.1099/00221287-143-2-303. Yeast-enhanced green fluorescent protein (yEGFP): a reporter of gene expression in Candida albicans

Yeast-enhanced green fluorescent protein (yEGFP): a reporter of gene expression in Candida albicans

10.1099/00221287-143-2-303

Crossref journal-article

Microbiology Society

Microbiology (345)

Abstract

The green fluorescent protein (GFP) of Aequorea victoria has been developed here as a reporter for gene expression and protein localization in Candida albicans. When wild-type (wt) GFP was expressed in C. albicans, it was not possible to detect fluorescence or a translation product for the wt protein. Since this was probably due in part to the presence of the non-canonical CTG serine codon in the Aequorea sequence, this codon was changed to the leucine codon TTG. C. albicans cells expressing this construct contained GFP mRNA but were non-fluorescent and contained no detectable translation product. Hence a codon-optimized GFP gene was constructed in which all of the 239 amino acids are encoded by optimal codons for C albicans. In this gene were also incorporated two previously identified mutations in the chromophore that increase GFP fluorescence. C. albicans cells expressing this yeast-enhanced GFP gene (yEGFP3) are fluorescent and contain GFP protein. yEGFP3 can be used as a versatile reporter of gene expression in C. albicans and Saccharomyces cerevisiae and the optimized GFP described here should have broad applications in these and other fungal species.

Bibliography

Cormack, B. P., Bertram, G., Egerton, M., Gow, N. A. R., Falkow, S., & Brown, A. J. P. (1997). Yeast-enhanced green fluorescent protein (yEGFP): a reporter of gene expression in Candida albicans. Microbiology, 143(2), 303â311.

Authors 6

Brendan P. Cormack (first)
Gwyneth Bertram (additional)
Mark Egerton (additional)
Neil A. R. Gow (additional)
Stanley Falkow (additional)
Alistair J. P. Brown (additional)

References 43 Referenced 491

10.1128/JB.178.18.5353-5360.1996 / J Bacterial / The Candida albicans HYR1 gene, which is activated in response to hyphal development, belongs to a gene family encoding yeast cell wall proteins. by Bailey (1996)
10.1016/0092-8674(95)90326-7 / Cell / Degradation of mRNA in eukaryotes. by Beelman (1995)
10.1002/(SICI)1097-0061(199602)12:2<115::AID-YEA889>3.0.CO;2-E / Yeast / Structure and regulation of the Candida albicans ADH1 gene encoding an immunogenic alcohol dehydrogenase. by Bertram (1996)
{'key': 'R4', 'first-page': '127', 'article-title': 'RNA extraction and mRNA quantitation in Candida albicans.', 'volume-title': 'In Molecular Biology of Pathogenic Fungi: a Laboratory Manual', 'author': 'Brown', 'year': '1994'} / In Molecular Biology of Pathogenic Fungi: a Laboratory Manual / RNA extraction and mRNA quantitation in Candida albicans. by Brown (1994)
10.1093/nar/19.15.4298 / Nucleic Acids Res / Codon utilisation in the pathogenic yeast, Candida albicans. by Brown (1991)
{'key': 'R6', 'first-page': '75', 'article-title': 'Stable transformation and regulated expression of an inducible reporter construct in Candida albicans using restriction enzyme-mediated integration.', 'volume': '251', 'author': 'Brown', 'year': '1996', 'journal-title': 'Mol Gen Genet'} / Mol Gen Genet / Stable transformation and regulated expression of an inducible reporter construct in Candida albicans using restriction enzyme-mediated integration. by Brown (1996)
10.1007/BF00279393 / Mol Gen Genet / Cloning and expression of Candida albicans ADE2 and proteinase genes on a replicative plasmid in C.albicans and in Saccharomyces cerevisiae. by Cannon (1992)
10.1126/science.8303295 / Science / Green fluorescent protein as a marker for gene expression. by Chalfie (1994)
10.1016/0378-1119(95)00685-0 / Gene / FACS optimized mutants of the green fluorescent protein (GFP). by Cormack (1996)
10.1016/S0968-0004(00)89099-4 / Trends Biochem Sci / Understanding, improving and using green fluorescent proteins. by Cubitt (1995)
10.1146/annurev.mi.45.100191.001155 / Annu Rev Microbiol / Putative virulence factors of Candida albicans. by Cutler (1991)
10.1016/0003-2697(83)90418-9 / Anal Biochem / A technique for radio-labeling DNA restriction endonuclease fragments to high specific activity. by Feinberg (1983)
10.1093/genetics/134.3.717 / Genetics / Isogenic strain construction and gene mapping in Candida albicans. by Fonzi (1993)
10.1128/JB.174.21.6992-6996.1992 / J Bacteriol / Cloning and characterization of a Candida albicans maltase gene involved in sucrose utilization. by Geber (1992)
10.1093/nar/20.6.1425 / Nucleic Acids Res / Improved method for high efficiency transformation of intact yeast cells. by Gietz (1992)
{'key': 'R16', 'volume-title': 'Guide to Yeast Genetics and Molecular Biology.', 'author': 'Guthrie', 'year': '1991'} / Guide to Yeast Genetics and Molecular Biology. by Guthrie (1991)
10.1073/pnas.91.26.12501 / Proc Natl Acad Sci USA / Wavelength mutations and posttranslational autoxidation of green fluorescent protein. by Heim (1994)
10.1093/infdis/161.4.761 / J Infect Dis / The iC3b receptor on Candida albicans: subcellular localization and modulation of receptor expression by glucose. by Hostetter (1990)
10.1128/JB.153.1.163-168.1983 / J Bacteriol / Trans-formation of intact yeast cells treated with alkali cations. by Ito (1983)
10.1128/MCB.6.1.142 / Mol Cell Biol / Integrative transformation of Candida albicans, using a cloned Candida ADE2 gene. by Kurtz (1986)
10.1038/227680a0 / Nature / Cleavage of structural proteins during the assembly of the head of bacteriophage T4. by Laemmli (1970)
10.1073/pnas.93.23.13217 / Proc Natl Acad Sci USA / Homologs of the Ste20p and Ste7p protein kinases are involved in hyphal formation of Candida albicans. by Leberer (1996)
10.1007/BF00279365 / Mol Gen Genet / β-Galactosidase of Kluyveromyces lactis (Lac4p) as reporter of gene expression in Candida albicans and C. tropicalis. by Leuker (1992)
10.1038/293311a0 / Nature / Regulation of protein synthesis during heat shock. by Lindquist (1981)
10.1093/nar/20.20.5289 / Nucleic Acids Res / Evolution of codon usage patterns: the extent and nature of divergence between Candida albicans and Saccharomyces cerevisiae. by Lloyd (1992)
10.1128/MCB.11.10.5330 / Mol Cell Biol / Yeast glycolytic mRNAs are differentially regulated. by Moore (1991)
10.1021/bi00709a028 / Biochemistry / Intermolecular energy transfer in the bioluminescent system of Aequorea. by Morise (1974)
10.1007/BF00326156 / Curr Genet / Use of URA3 as a reporter of gene expression in C.albicans. by Myers (1995)
{'key': 'R29', 'volume-title': 'Candida and Candidosis', 'author': 'Odds', 'year': '1988'} / Candida and Candidosis by Odds (1988)
{'key': 'R30', 'first-page': '313', 'article-title': 'Candida species and virulence.', 'volume': '60', 'author': 'Odds', 'year': '1994', 'journal-title': 'ASM News'} / ASM News / Candida species and virulence. by Odds (1994)
10.1093/nar/21.17.4039 / Nucleic Acids Res / Non-universal decoding of the leucine codon CUG in several Candida species. by Ohama (1993)
10.1016/0378-1119(92)90691-H / Gene / Primary structure of the Aequorea victoria green-fluorescent protein. by Prasher (1992)
10.1128/MR.59.3.423-450.1995 / Microbiol Rev / mRNA stability in mammalian cells. by Ross (1995)
10.1073/pnas.74.12.5463 / Proc Natl Acad Sci USA / DNA sequencing with chain-terminating inhibitors. by Sanger (1977)
10.1093/nar/23.9.1481 / Nucleic Acids Res / The CUG codon is decoded in vivo as serine and not leucine in Candida albicans. by Santos (1995)
10.1002/yea.320100507 / Yeast / Transfer RNA profiling: a new method for the identification of pathogenic Candida species. by Santos (1994)
10.1128/MR.54.3.226-241.1990 / Microbiol Rev / Genetics of Candida albicans. by Scherer (1990)
10.1091/mbc.6.12.1641 / Mol Biol Cell / Cell cycle-regulated phosphorylation of Swi6 controls its nuclear localization. by Sidorova (1995)
10.1128/JB.178.1.121-129.1996 / J Bacteriol / The sea pansy Renilla reniformis luciferase serves as a sensitive bioluminescent reporter for differential gene expression in Candida albicans. by Srikantha (1996)
10.1016/0092-8674(89)90584-9 / Cell / Elevated recombination rates in transcriptionally active DNA. by Thomas (1989)
10.1038/369400a0 / Nature / Implications for bcd mRNA localization from spatial distribution of exu protein in Drosophila oogenesis. by Wang (1994)
10.1111/j.1751-1097.1980.tb03755.x / Photochem Photobiol / Spectrophotometric identity of the energy-transfer chromophores in Renilla and Aequorea green fluorescent proteins. by Ward (1980)
10.1128/JB.177.10.2953-2955.1995 / J Bacteriol / The ̒universal̓ leucine codon CTG in the secreted aspartyl proteinase 1 (SAP1) gene of Candida albicans encodes a serine in vivo. by White (1995)

Dates

Type	When
Created	16 years, 2 months ago (June 5, 2009, 12:53 a.m.)
Deposited	4 years, 5 months ago (March 10, 2021, 10:22 a.m.)
Indexed	1 month ago (July 20, 2025, 12:26 a.m.)
Issued	28 years, 6 months ago (Feb. 1, 1997)
Published	28 years, 6 months ago (Feb. 1, 1997)
Published Print	28 years, 6 months ago (Feb. 1, 1997)

Funders 0

None

BibTeX

@article{Cormack_1997, title={Yeast-enhanced green fluorescent protein (yEGFP): a reporter of gene expression in Candida albicans}, volume={143}, ISSN={1465-2080}, url={http://dx.doi.org/10.1099/00221287-143-2-303}, DOI={10.1099/00221287-143-2-303}, number={2}, journal={Microbiology}, publisher={Microbiology Society}, author={Cormack, Brendan P. and Bertram, Gwyneth and Egerton, Mark and Gow, Neil A. R. and Falkow, Stanley and Brown, Alistair J. P.}, year={1997}, month=feb, pages={303–311} }

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      "article-title": "Structure and regulation of the Candida albicans ADH1 gene encoding an immunogenic alcohol dehydrogenase.",
      "volume": "12",
      "author": "Bertram",
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      "journal-title": "Yeast"
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      "key": "R4",
      "first-page": "127",
      "article-title": "RNA extraction and mRNA quantitation in Candida albicans.",
      "volume-title": "In Molecular Biology of Pathogenic Fungi: a Laboratory Manual",
      "author": "Brown",
      "year": "1994"
    },
    {
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      "article-title": "Codon utilisation in the pathogenic yeast, Candida albicans.",
      "volume": "19",
      "author": "Brown",
      "year": "1991",
      "journal-title": "Nucleic Acids Res"
    },
    {
      "key": "R6",
      "first-page": "75",
      "article-title": "Stable transformation and regulated expression of an inducible reporter construct in Candida albicans using restriction enzyme-mediated integration.",
      "volume": "251",
      "author": "Brown",
      "year": "1996",
      "journal-title": "Mol Gen Genet"
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      "first-page": "453",
      "DOI": "10.1007/BF00279393",
      "article-title": "Cloning and expression of Candida albicans ADE2 and proteinase genes on a replicative plasmid in C.albicans and in Saccharomyces cerevisiae.",
      "volume": "235",
      "author": "Cannon",
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      "article-title": "Green fluorescent protein as a marker for gene expression.",
      "volume": "263",
      "author": "Chalfie",
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      "journal-title": "Science"
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      "doi-asserted-by": "crossref",
      "first-page": "33",
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      "author": "Cormack",
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      "journal-title": "Gene"
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      "article-title": "A technique for radio-labeling DNA restriction endonuclease fragments to high specific activity.",
      "volume": "132",
      "author": "Feinberg",
      "year": "1983",
      "journal-title": "Anal Biochem"
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    {
      "key": "R13",
      "doi-asserted-by": "crossref",
      "first-page": "717",
      "DOI": "10.1093/genetics/134.3.717",
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      "year": "1993",
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      "author": "Gietz",
      "year": "1992",
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    },
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      "key": "R16",
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      "author": "Guthrie",
      "year": "1991"
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      "key": "R17",
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      "first-page": "12501",
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      "author": "Hostetter",
      "year": "1990",
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