DOI: 10.1098/rstb.1999.0406. Tobacco mosiac virus particle structure and the initiation of disassembly

Tobacco mosiac virus particle structure and the initiation of disassembly

10.1098/rstb.1999.0406

Crossref journal-article

The Royal Society

Philosophical Transactions of the Royal Society of London. Series B: Biological Sciences (175)

Abstract

The structure of an intact tobacco mosaic virus (TMV) particle was determined at 2.9 Å resolution using fibre diffraction methods. All residues of the coat protein and the three nucleotides of RNA that are bound to each protein subunit were visible in the electron density map. Examination of the structures of TMV, cucumber green mottle mosaic virus and ribgrass mosaic virus, and site–directed mutagenesis experiments in which carboxylate groups were changed to the corresponding amides, showed that initial stages of disassembly are driven by complex electrostatic interactions involving at least seven carboxylate side–chains and a phosphate group. The locations of these interactions can drift during evolution, allowing the viruses to evade plant defensive responses that depend on recognition of the viral coat protein surface.

Bibliography

Stubbs, G. (1999). Tobacco mosiac virus particle structure and the initiation of disassembly. Philosophical Transactions of the Royal Society of London. Series B: Biological Sciences, 354(1383), 551â557.

Authors 1

Gerald Stubbs (first)

References 9 Referenced 61

Barrett A. N. Barrington Leigh J. Holmes K. C. Leberman R. Mandelkow E. & von Sengbusch P. 1971 An electron density map of tobacco mosaic virus at 10 Ð resolution. Cold Spring Harbor Symp. Quant. Biol. 36 1433^448. (10.1101/SQB.1972.036.01.056)
Bawden F. C. Pirie N. W. Bernal J. D. & Fankuchen I. 1936 Liquid crystalline substances from virus-infected plants. Nature 138 1051^1053. (10.1038/1381051a0)
{'key': 'p_3', 'article-title': 'X-ray and crystallographic studies of plant virus preparations', 'volume': '28', 'author': 'Bernal J. D.', 'year': '1941', 'journal-title': 'J. Gen. Physiol.'} / J. Gen. Physiol. / X-ray and crystallographic studies of plant virus preparations by Bernal J. D. (1941)
{'key': 'p_4', 'first-page': '61', 'article-title': 'Crystallographic re¢nement by simulated annealing: application to crambin', 'volume': '45', 'author': 'BrÏnger A. T.', 'year': '1989', 'journal-title': 'Acta Cryst. A'} / Acta Cryst. A / Crystallographic re¢nement by simulated annealing: application to crambin by BrÏnger A. T. (1989)
10.1016/0022-2836(72)90063-0
Caspar D. L. D. 1956 Radial density distribution in the tobacco mosaic virus particle. Nature 177 928^928. (10.1038/177928a0)
{'key': 'p_7', 'first-page': '121', 'article-title': 'Assembly and stability of the tobacco mosaic virus particle', 'volume': '18', 'author': 'Caspar D. L. D.', 'year': '1963', 'journal-title': 'Adv. Protein Chem.'} / Adv. Protein Chem. / Assembly and stability of the tobacco mosaic virus particle by Caspar D. L. D. (1963)
10.1006/jmbi.1994.1564
Culver J. N. Dawson W. O. Plonk K. & Stubbs G. 1995 Sitedirected mutagenesis con¢rms the involvement of carboxylate groups in the disassembly of tobacco mosaic virus. Virology 206 724^730. (10.1016/S0042-6822(95)80096-4)

Dates

Type	When
Created	23 years, 1 month ago (July 26, 2002, 7:55 p.m.)
Deposited	4 years, 6 months ago (Feb. 14, 2021, 4:39 p.m.)
Indexed	1 month, 1 week ago (July 16, 2025, 8:15 a.m.)
Issued	26 years, 4 months ago (March 29, 1999)
Published	26 years, 4 months ago (March 29, 1999)
Published Online	26 years, 4 months ago (March 29, 1999)
Published Print	26 years, 4 months ago (March 29, 1999)

Funders 0

None

BibTeX

@article{Stubbs_1999, title={Tobacco mosiac virus particle structure and the initiation of disassembly}, volume={354}, ISSN={1471-2970}, url={http://dx.doi.org/10.1098/rstb.1999.0406}, DOI={10.1098/rstb.1999.0406}, number={1383}, journal={Philosophical Transactions of the Royal Society of London. Series B: Biological Sciences}, publisher={The Royal Society}, author={Stubbs, Gerald}, editor={Harrison, B. D. and Wilson, T. M. A.}, year={1999}, month=mar, pages={551–557} }

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