DOI: 10.1098/rstb.1999.0404. The tobacco mosaic virus particle: structure and assembly

The tobacco mosaic virus particle: structure and assembly

10.1098/rstb.1999.0404

Crossref journal-article

The Royal Society

Philosophical Transactions of the Royal Society of London. Series B: Biological Sciences (175)

Abstract

A short account is given of the physical and chemical studies that have led to an understanding of the structure of the tobacco mosaic virus particle and how it is assembled from its constituent coat protein and RNA. The assembly is a much more complex process than might have been expected from the simplicity of the helical design of the particle. The protein forms an obligatory intermediate (a cylindrical disk composed of two layers of protein units), which recognizes a specific RNA hairpin sequence. This extraordinary mechanism simultaneously fulfils the physical requirement for nucleating the growth of the helical particle and the biological requirement for specific recognition of the viral DNA.

Bibliography

Klug, A. (1999). The tobacco mosaic virus particle: structure and assembly. Philosophical Transactions of the Royal Society of London. Series B: Biological Sciences, 354(1383), 531â535.

Authors 1

A. Klug (first)

References 20 Referenced 441

Bloomer A. C. Champness J. N. Bricogne G. Staden R. & Klug A. 1978 Protein disk of tobacco mosaic virus at 2.8 Ð resolution showing the interactions within and between subunits. Nature 276 362^368. (10.1038/276362a0)
10.1038/newbio229047a0
Butler P. J. G. Bloomer A. C. Bricogne G. Champness J. N. Graham J. Guilley H. Klug A. & Zimmern D. 1976 Tobacco mosaic virus assemblyöspeci¢city and the transition in protein structure during RNA packaging. In Structure ^function relationships of proteins 3rd John Innes Symposium (ed. R. Markham & R. W. Horne) pp.101^110. Amsterdam: North Holland Publishing Co.
10.1016/0022-2836(92)91002-7
{'key': 'p_5', 'first-page': '121', 'article-title': 'Assembly and stability of the tobacco mosaic virus particle', 'volume': '18', 'author': 'Caspar D. L. D.', 'year': '1963', 'journal-title': 'Adv. Prot. Chem.'} / Adv. Prot. Chem. / Assembly and stability of the tobacco mosaic virus particle by Caspar D. L. D. (1963)
Caspar D. L. D. & Namba K. 1990 Switching in the selfassembly of tobacco mosaic virus. Adv. Biophys. 26 157^185. (10.1016/0065-227X(90)90011-H)
10.1016/S0006-3495(98)77818-X
10.1016/0014-5793(72)80473-3
{'key': 'p_9', 'first-page': '46', 'article-title': 'Polymerisation of tobacco mosaic virus protein and its control', 'volume': '229', 'author': 'Durham A. C. H.', 'year': '1971', 'journal-title': 'Nature New Biol.'} / Nature New Biol. / Polymerisation of tobacco mosaic virus protein and its control by Durham A. C. H. (1971)
10.1038/newbio229042a0
Klug A. 1979 The assembly of tobacco mosaic virus: structure and speci¢city.The Harvey Lecture 74 141^162.
{'key': 'p_12', 'first-page': '63', 'article-title': 'The structure of simple viruses', 'volume': '13', 'author': 'Klug A.', 'year': '1960', 'journal-title': 'Adv.Virus Res.'} / Adv.Virus Res. / The structure of simple viruses by Klug A. (1960)
Namba K. & Stubbs G. 1986 Structure of tobacco mosaic virus at 3.6 Ð resolution: implications for assembly. Science 231 1401^1406. (10.1126/science.3952490)
{'key': 'p_14', 'article-title': 'Visualisation of protein^ nucleic acid interactions in a virus. Re¢ned structure of intact tobacco mosaic virus at 2.9 Ð resolution by X-ray ¢ber di¡raction', 'volume': '208', 'author': 'Namba K.', 'year': '1989', 'journal-title': 'J. Mol. Biol.'} / J. Mol. Biol. / Visualisation of protein^ nucleic acid interactions in a virus. Re¢ned structure of intact tobacco mosaic virus at 2.9 Ð resolution by X-ray ¢ber di¡raction by Namba K. (1989)
10.1016/0022-2836(75)90169-2
Stubbs G. Warren S. & Holmes K. 1977 Structure of RNA and RNA binding site in tobacco mosaic virus from a 4-Ð map calculated from X-ray ¢bre diagrams. Nature 267 216^ 221. (10.1038/267216a0)
10.1016/0022-2836(74)90075-8
Zimmern D. 1977 Nucleotide sequence at the origin for assembly on tobacco mosaic virus RNA. Cell 11 463^482. (10.1016/0092-8674(77)90065-4)
Zimmern D. & Butler P. J. G. 1977 The isolation of tobacco mosaic virus RNA fragments containing the origin for viral assembly. Cell 11 455^462. (10.1016/0092-8674(77)90064-2)
10.1016/0014-5793(76)80954-4

Dates

Type	When
Created	23 years, 1 month ago (July 26, 2002, 7:55 p.m.)
Deposited	4 years, 6 months ago (Feb. 14, 2021, 4:38 p.m.)
Indexed	1 day, 13 hours ago (Aug. 27, 2025, 12:26 p.m.)
Issued	26 years, 5 months ago (March 29, 1999)
Published	26 years, 5 months ago (March 29, 1999)
Published Online	26 years, 5 months ago (March 29, 1999)
Published Print	26 years, 5 months ago (March 29, 1999)

Funders 0

None

BibTeX

@article{Klug_1999, title={The tobacco mosaic virus particle: structure and assembly}, volume={354}, ISSN={1471-2970}, url={http://dx.doi.org/10.1098/rstb.1999.0404}, DOI={10.1098/rstb.1999.0404}, number={1383}, journal={Philosophical Transactions of the Royal Society of London. Series B: Biological Sciences}, publisher={The Royal Society}, author={Klug, A.}, editor={Harrison, B. D. and Wilson, T. M. A.}, year={1999}, month=mar, pages={531–535} }

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