DOI: 10.1098/rstb.1995.0049. Nascent chains: folding and chaperone interaction during elongation on ribosomes

Nascent chains: folding and chaperone interaction during elongation on ribosomes

10.1098/rstb.1995.0049

Crossref journal-article

The Royal Society

Philosophical Transactions of the Royal Society of London. Series B: Biological Sciences (175)

Abstract

Monoclonal antibodies that detect folding intermediates in vitro were used to monitor the appearance of folded polypeptide chains during their synthesis on the ribosomes. Nascent immunoreactive chains of the bacteriophage P22 tail-spike protein and of the Escherichia coli β 2 subunit of tryptophan-synthase were thus identified, suggesting that they can fold on the ribosomes. Moreover, the immunoreactivity of ribosome- bound tryptophan-synthase β-chains of intermediate lengths was shown to appear with no detectable delay compared to their synthesis. This suggested that β-chains start folding during their elongation on the ribosomes. However, newly synthesized incomplete β-chains were shown to interact with chaperones while still bound to the ribosome. Because of the peculiar properties of the epitope recognized by the anti- tryptophan-synthase monoclonal antibody used, it could not be concluded whether the immunoreactivity of the nascent β-chains resulted from their ability to fold cotranslationally or from their association with chaperones which might maintain them in an unfolded, immunoreactive state.

Bibliography

Nascent chains: folding and chaperone interaction during elongation on ribosomes. (1995). Philosophical Transactions of the Royal Society of London. Series B: Biological Sciences, 348(1323), 89â95.

Authors 0

None

References 12 Referenced 16

10.1073/pnas.68.2.390
10.1021/bi00461a026
{'key': 'p_3', 'first-page': '351', 'article-title': 'Folding on the ribosome of Escherichia coli tryptophan synthase P subunit nascent chains probed with a conform ation-dependent monoclonal antibody. J. molec', 'volume': '228', 'author': 'Fedorov A.N.', 'year': '1992', 'journal-title': 'Biol.'} / Biol. / Folding on the ribosome of Escherichia coli tryptophan synthase P subunit nascent chains probed with a conform ation-dependent monoclonal antibody. J. molec by Fedorov A.N. (1992)
{'key': 'p_4', 'first-page': '10347', 'article-title': 'Properties of M onoclonal Antibodies Selected for Probing the Conform ation of Wild Type and M u tan t Forms of the P22 Tailspike Endorham nosidase. J . biol', 'volume': '265', 'author': 'Friguet B.', 'year': '1990', 'journal-title': 'Chem.'} / Chem. / Properties of M onoclonal Antibodies Selected for Probing the Conform ation of Wild Type and M u tan t Forms of the P22 Tailspike Endorham nosidase. J . biol by Friguet B. (1990)
10.1006/abio.1993.1206
{'key': 'p_6', 'first-page': '15945', 'article-title': 'In vitro and Ribosom e-bound Folding Interm ediates of P22 Tailspike Protein Detected with M onoclonal Antibodies J. biol', 'volume': '269', 'author': 'Friguet B.', 'year': '1994', 'journal-title': 'Chem.'} / Chem. / In vitro and Ribosom e-bound Folding Interm ediates of P22 Tailspike Protein Detected with M onoclonal Antibodies J. biol by Friguet B. (1994)
10.1016/0014-5793(90)80703-L
10.1073/pnas.79.11.3403
{'key': 'p_9', 'first-page': '4977', 'article-title': 'Form ation of aggregates from a thermolabile in vivo folding interm ediate in P22 tailspike renaturation. J. biol', 'volume': '263', 'author': 'Haase-Pettingel C.A.', 'year': '1988', 'journal-title': 'Chem.'} / Chem. / Form ation of aggregates from a thermolabile in vivo folding interm ediate in P22 tailspike renaturation. J. biol by Haase-Pettingel C.A. (1988)
10.1016/0003-2697(79)90721-8
10.1021/bi00420a010
{'key': 'p_12', 'first-page': '11750', 'article-title': 'Reconstitution of the thermostable trimeric phage P22 tailspike protein from denatured chains in vitro. J. biol', 'volume': '264', 'author': 'Seckler R.', 'year': '1989', 'journal-title': 'Chem.'} / Chem. / Reconstitution of the thermostable trimeric phage P22 tailspike protein from denatured chains in vitro. J. biol by Seckler R. (1989)

Dates

Type	When
Created	18 years, 8 months ago (Dec. 18, 2006, 7:03 p.m.)
Deposited	4 years, 6 months ago (Feb. 18, 2021, 3:28 p.m.)
Indexed	1 year, 9 months ago (Oct. 29, 2023, 6:44 a.m.)
Issued	30 years, 3 months ago (April 29, 1995)
Published	30 years, 3 months ago (April 29, 1995)
Published Online	28 years, 7 months ago (Jan. 1, 1997)
Published Print	30 years, 3 months ago (April 29, 1995)

Funders 0

None

BibTeX

@article{1995, volume={348}, ISSN={1471-2970}, url={http://dx.doi.org/10.1098/rstb.1995.0049}, DOI={10.1098/rstb.1995.0049}, number={1323}, journal={Philosophical Transactions of the Royal Society of London. Series B: Biological Sciences}, publisher={The Royal Society}, year={1995}, month=apr, pages={89–95} }

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