DOI: 10.1098/rstb.1989.0008. Fluorescence analysis during steady-state photosynthesis

Fluorescence analysis during steady-state photosynthesis

10.1098/rstb.1989.0008

Crossref journal-article

The Royal Society

Philosophical Transactions of the Royal Society of London. B, Biological Sciences (175)

Abstract

Photosynthetic gas exchange of attached leaves has been measured under steady-state conditions at different light intensities and correlated with simultaneous measurements of chlorophyll fluorescence and oxidized P 700 (by absorbance changes at 820 nm). The data suggest that during light-saturated assimilation, photosystem II (PSII) photochemistry is mainly controlled by non-photochemical and non-radiative dissipation of excitation energy, rather than by accumulation of reduced acceptor, Q A , and this could be related to ‘high-energy quenching’ of fluorescence. The occurrence of oxidized P 700 at saturating light and low concentration of CO 2 suggests that in the steady state PSI photochemistry is controlled by a shortage of electron donation from the plastoquinone pool (photosynthetic control), rather than by excess electrons at the acceptor side. The significance of the oxidized form of P 700 as a ‘quencher’ of excitation energy is discussed. This control of photosystems I and II, both related to the proton gradient across the thylakoid membrane, may serve to match the potential rate of net photochemistry to the demand by the biochemical reactions. However, when light-saturated assimilation is not limited by CO 2 , PSI activity is controlled by accumulation of reduced electron acceptors, rather than by photosynthetic mechanisms. Photosynthetic control has been found to determine the redox state of the ferredoxin-thioredoxin system.

Bibliography

Fluorescence analysis during steady-state photosynthesis. (1989). Philosophical Transactions of the Royal Society of London. B, Biological Sciences, 323(1216), 253â268.

Authors 0

None

References 37 Referenced 59

10.1007/BF00402983
{'key': 'p_2', 'first-page': '542', 'article-title': 'Analysis of the slow phases of the in vivo chlorophyll fluorescence induction curve. Changes in the redox state of photosystem II electron acceptors and fluorescence emission from photosystems I and II. Biochim. biophys', 'volume': '635', 'author': 'Bradbury M.', 'year': '1981', 'journal-title': 'Acta'} / Acta / Analysis of the slow phases of the in vivo chlorophyll fluorescence induction curve. Changes in the redox state of photosystem II electron acceptors and fluorescence emission from photosystems I and II. Biochim. biophys by Bradbury M. (1981)
Briantais J.-M . Vernotte C. Krause G. H. & Weis E. 1986 Chlorophyll a fluorescence of higher plants: chloroplasts and leaves. In Light emission by plants and bacteria (ed. Govindjee J. Amesz & D. A. Fork) pp. 539-583. Orlando Florida: Academic Press. (10.1016/B978-0-12-294310-2.50024-X)
{'key': 'p_4', 'first-page': '43', 'article-title': 'Regulation of the formation and utilization of photosynthate in leaves. Biochim. biophys', 'volume': '853', 'author': 'Cseke C.', 'year': '1986', 'journal-title': 'Acta'} / Acta / Regulation of the formation and utilization of photosynthate in leaves. Biochim. biophys by Cseke C. (1986)
10.1007/BF00391092
10.1007/BF00397352
{'key': 'p_7', 'first-page': '552', 'article-title': 'Regulation of photosynthesis in isolated spinach chloroplasts during orthophosphate limitation. Biochim. biophys', 'volume': '894', 'author': 'Furbank R. T.', 'year': '1987', 'journal-title': 'Acta.'} / Acta. / Regulation of photosynthesis in isolated spinach chloroplasts during orthophosphate limitation. Biochim. biophys by Furbank R. T. (1987)
10.1007/BF00032706
Graber P. Junesch U. & Thulke G. 1987 The chloroplast ATP-synthase: the rate of the catalytic reaction. In Progress in photosynthesis research vol. 3 (ed. J. Biggins) pp. 177-184. Dordrecht: Martinus Nijhoff. (10.1007/978-94-017-0516-5_39)
Harbinson J. & Woodward I. 1987 The use of light-induced absorbance changes at 820 nm to monitor the oxidation state of P700 in plants. Plant Cell <Sc Environm. 10 131-140. (10.1111/1365-3040.ep11602090)
Heber U. Enser U. Weis E. Ziem U. & Giersch C. 1979 Regulation of the photosynthetic carbon cycle phosphorylation and electron transport in illuminated intact chloroplasts. In UCLA-Symposia (ed. D. E. Atkinson) pp. 113-137. New York: Academic Press. (10.1016/B978-0-12-066250-0.50012-0)
{'key': 'p_12', 'first-page': '144', 'article-title': 'The assimilatory power as driving force in photosynthesis. Biochim. biophys', 'volume': '852', 'author': 'Heber U.', 'year': '1986', 'journal-title': 'Acta'} / Acta / The assimilatory power as driving force in photosynthesis. Biochim. biophys by Heber U. (1986)
Horton P. 1987 Interplay between environmental and metabolic factors in the regulation of electron transport in higher plants. In Progress Nijhoff. (10.1007/978-94-009-3535-8_161)
inphotosynthesis research vol. 2 (ed. J. Biggins) pp. 681-688. Dordrecht: Martinus
{'key': 'p_15', 'first-page': '107', 'article-title': 'Studies on the induction of chlorophyll fuorescence in isolated barley protoplasts. VI. Resolution of non-photochemical quenching. Biochim. biophys', 'volume': '932', 'author': 'Horton P.', 'year': '1988', 'journal-title': 'Acta'} / Acta / Studies on the induction of chlorophyll fuorescence in isolated barley protoplasts. VI. Resolution of non-photochemical quenching. Biochim. biophys by Horton P. (1988)
{'key': 'p_16', 'first-page': '453', 'article-title': 'Regulation of the photochemical efficiency of photosystem 2; consequences for the light response of field photosynthesis', 'volume': '26', 'author': 'Horton P.', 'year': '1988', 'journal-title': 'PI. Physiol. Biochem.'} / PI. Physiol. Biochem. / Regulation of the photochemical efficiency of photosystem 2; consequences for the light response of field photosynthesis by Horton P. (1988)
{'key': 'p_17', 'first-page': '317', 'article-title': 'New evidence supporting energy transfer between photosynthetic units. Biochim biophys', 'volume': '305', 'author': 'Joliot P.', 'year': '1973', 'journal-title': 'Acta'} / Acta / New evidence supporting energy transfer between photosynthetic units. Biochim biophys by Joliot P. (1973)
{'key': 'p_18', 'first-page': '183', 'article-title': 'The rise time of photoreduction, difference spectrum and oxidation reduction potential of P430', 'volume': '82', 'author': 'Ke B.', 'year': '1972', 'journal-title': 'Archs Biochem. Biophys.'} / Archs Biochem. Biophys. / The rise time of photoreduction, difference spectrum and oxidation reduction potential of P430 by Ke B. (1972)
{'key': 'p_19', 'first-page': '116', 'article-title': 'Photoinduced quenching of chlorophyll fluorescence in intact chloroplasts and algae. Biochim. biophys', 'volume': '679', 'author': 'Krause G. H.', 'year': '1982', 'journal-title': 'Acta'} / Acta / Photoinduced quenching of chlorophyll fluorescence in intact chloroplasts and algae. Biochim. biophys by Krause G. H. (1982)
{'key': 'p_20', 'first-page': '169', 'article-title': 'Characterization of chlorophyll fluorescence quenching in chloroplasts by fluorescence spectroscopy at 77 K. I. ApH-dependent quenching. Biochim. biophys', 'volume': '723', 'author': 'Krause G. H.', 'year': '1983', 'journal-title': 'Acta'} / Acta / Characterization of chlorophyll fluorescence quenching in chloroplasts by fluorescence spectroscopy at 77 K. I. ApH-dependent quenching. Biochim. biophys by Krause G. H. (1983)
Krause G. H. & Laasch H. 1987 Photoinhibition of photosynthesis. Studies on mechanisms of damage and protection in chloroplasts. In Progress in photosynthesis Martinus Nijhoff. (10.1007/978-94-017-0519-6_3)
vol. 4 (ed. J. Biggins) pp. 19-26. Dordrecht :
{'key': 'p_23', 'first-page': '445', 'article-title': 'Regulation of thermal energy dissipation of absorbed light energy in chloroplasts indicated by energy-dependent fluorescence quenching', 'volume': '26', 'author': 'Krause G. H.', 'year': '1988', 'journal-title': 'PI. Physiol. Biochem.'} / PI. Physiol. Biochem. / Regulation of thermal energy dissipation of absorbed light energy in chloroplasts indicated by energy-dependent fluorescence quenching by Krause G. H. (1988)
10.1007/BF00028527
Kyle D. J. 1987 The biochemical basis for photoinhibition of photosystem II. In Topics in photosynthesis (ed. D. J. Kyle C. B. Osmond & C. J. Arntzen) vol. 9 ( ) pp. 197-226. Amsterdam: Elsevier.
10.1098/rspb.1986.0024
10.1016/0014-5793(74)80997-X
{'key': 'p_28', 'first-page': '310', 'article-title': 'Picosecond absorbancedifference spectroscopy on the primary reactions and the antenna-excited states in photosystem I particles. Biochim. biophys', 'volume': '850', 'author': 'Nuijs A. M.', 'year': '1986', 'journal-title': 'Acta'} / Acta / Picosecond absorbancedifference spectroscopy on the primary reactions and the antenna-excited states in photosystem I particles. Biochim. biophys by Nuijs A. M. (1986)
10.1111/j.1399-3054.1987.tb04362.x
{'key': 'p_30', 'first-page': '23', 'article-title': 'pH dependence of oxygen evolution and reduction kinetics of photooxidized chlorophyll <zn (P-680) in photosystem II particles from Synechococcus sp. Biochim. biophys', 'volume': '890', 'author': 'Schlodder E.', 'year': '1987', 'journal-title': 'Acta'} / Acta / pH dependence of oxygen evolution and reduction kinetics of photooxidized chlorophyll <zn (P-680) in photosystem II particles from Synechococcus sp. Biochim. biophys by Schlodder E. (1987)
10.1007/BF00024185
Schreiber U. & Neubauer C. 1987 The polyphasic rise of chlorophyll fluorescence upon onset of strong continuous illumination: II. Partial control by the photosystem II donor side and possible ways of interpretation. Z. Naturf. 42c 1255-1264. (10.1515/znc-1987-11-1218)
10.1111/j.1432-1033.1972.tb01667.x
10.1111/j.1469-8137.1986.tb00826.x
10.1104/pp.81.4.1115
Tran-Ahn T. & Rumberg B. 1987 Coupling mechanism between proton transport and ATP synthesis in chloroplasts. In Progress in photosynthesis Nijhoff. (10.1007/978-94-017-0516-5_40)
research vol. 3 (ed. J. Biggins) pp. 185-188. Dordrecht: Martinus

Dates

Type	When
Created	18 years, 8 months ago (Dec. 18, 2006, 5:49 p.m.)
Deposited	4 years, 6 months ago (Feb. 20, 2021, 8:16 p.m.)
Indexed	2 weeks, 5 days ago (Aug. 6, 2025, 9:45 a.m.)
Issued	36 years, 4 months ago (April 12, 1989)
Published	36 years, 4 months ago (April 12, 1989)
Published Online	28 years, 7 months ago (Jan. 1, 1997)
Published Print	36 years, 4 months ago (April 12, 1989)

Funders 0

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BibTeX

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