DOI: 10.1094/phyto.2003.93.4.402. Cytological Evidence of an Active Role of Silicon in Wheat Resistance to Powdery Mildew (<i>Blumeria graminis</i> f. sp. <i>tritici</i>)

Cytological Evidence of an Active Role of Silicon in Wheat Resistance to Powdery Mildew (Blumeria graminis f. sp. tritici)

10.1094/phyto.2003.93.4.402

Crossref journal-article

Scientific Societies

Phytopathology® (1665)

Abstract

Silicon (Si) amendments in the form of exogenously supplied nutrient solution or calcium silicate slag protect wheat plants from powdery mildew disease caused by the fungus Blumeria graminis f. sp. tritici. The most striking difference between Si- and Si+ plants challenged with B. graminis f. sp. tritici was the extent of epidermal cell infection and colonization by B. graminis f. sp. tritici. Histological and ultrastructural analyses revealed that epidermal cells of Si+ plants reacted to B. graminis f. sp. tritici attack with specific defense reactions including papilla formation, production of callose, and release of electron-dense osmiophilic material identified by cytochemical labeling as glycosilated phenolics. Phenolic material not only accumulated along the cell wall but also was associated with altered integrity of haustoria in a manner similar to localized phytoalexins as reported from other pathosystems. These results strongly suggest that Si mediates active localized cell defenses against B. graminis f. sp. tritici attack.

Bibliography

BÃ©langer, R. R., Benhamou, N., & Menzies, J. G. (2003). Cytological Evidence of an Active Role of Silicon in Wheat Resistance to Powdery Mildew (Blumeria graminis f. sp. tritici). PhytopathologyÂ®, 93(4), 402â412.

Authors 3

R. R. Bélanger (first)
Nicole Benhamou (additional)
J. G. Menzies (additional)

References 43 Referenced 263

10.1177/35.10.3114363
Benhamou, N. 1989. Preparation and application of lectin-gold complexes. Pages 205-218 in: Colloidal Gold, Principles, Methods and Applications, Vol. I. J. R. Latgé and D. Boucias, eds. Springer-Verlag, Berlin. (10.1016/B978-0-12-333927-0.50009-X)
10.1094/Phyto-82-468
10.1046/j.1365-313X.1998.00088.x
10.1139/m87-070
10.1094/Phyto-84-1432
10.1016/0885-5765(87)90012-9
10.1094/Phyto-84-236
10.1016/0885-5765(92)90053-X
10.1016/0885-5765(92)90022-N
Datnoff, L. E., Snyder, G. H., and Korndörfer, G. H. 2001. Silicon in Agriculture. Elsevier Science B.V., Amsterdam.
Deren, C. W. 2001. Plant genotype, silicon concentration, and siliconrelated responses. Pages 149-158 in: Silicon in Agriculture. L. E. Datnoff, G. H. Snyder, and G. H. Korndörfer, eds. Elsevier Science B.V., Amsterdam. (10.1016/S0928-3420(01)80012-4)
10.1073/pnas.91.1.11
Epstein, E. 2001. Silicon in plants: Facts vs. concepts. Pages 1-15 in: Silicon in Agriculture. L. E. Datnoff, G. H. Snyder, and G. H. Korndörfer, eds. Elsevier Science B.V., Amsterdam. (10.1016/S0928-3420(01)80005-7)
10.1094/PHYTO.1998.88.5.396
Fawe, A., Menzies, J. G., Chérif, M., and Bélanger, R. R. 2001. Silicon and disease resistance in dicotyledons. Pages 159-169 in: Silicon in Agriculture. L. E. Datnoff, G. H. Snyder, and G. H. Korndörfer, eds. Elsevier Science B.V., Amsterdam. (10.1016/S0928-3420(01)80013-6)
10.1038/physci241020a0
10.1111/j.1439-0329.1986.tb01049.x
{'key': 'p_19', 'first-page': '25', 'volume': '14', 'author': 'Germar B.', 'year': '1935', 'journal-title': 'Rev. Appl. Mycol.'} / Rev. Appl. Mycol. by Germar B. (1935)
10.1007/BF01332648
10.1080/00380768.1995.10419563
10.1080/00380768.1995.10419564
Ishiguro, K. 2001. Review of research in Japan on the roles of silicon in conferring resistance against rice blast. Pages 277-291 in: Silicon in Agriculture. L. E. Datnoff, G. H. Snyder, and G. H. Korndörfer, eds. Elsevier Science B.V., Amsterdam. (10.1016/S0928-3420(01)80021-5)
10.1016/S0065-2113(08)60734-8
10.1016/S0885-5765(88)80033-X
10.1016/0048-4059(75)90094-6
10.3186/jjphytopath.42.30
10.1016/0048-4059(76)90011-4
10.3186/jjphytopath.41.33
{'key': 'p_30', 'first-page': '154', 'volume': '96', 'author': 'Leusch H.-J.', 'year': '1989', 'journal-title': 'Z. Pflanzenkr. Pflanzensh.'} / Z. Pflanzenkr. Pflanzensh. by Leusch H.-J. (1989)
Marschner, H. 1986. Beneficial mineral elements. Silicon. Pages 351-359 in: Mineral Nutrition of Higher Plants. H. Marschner, ed. Academic Press, London.
10.1016/0885-5765(91)90007-5
10.1080/00380768.1983.10432407
10.1016/S0176-1617(87)80028-7
10.1139/b91-020
10.1016/S0885-5765(05)80027-X
10.1139/b86-103
Thordal-Christensen, H., Gregersen, P. L., and Collinge, D. 2000. The barley/Blumeria (syn. Erysiphe) graminis interaction. Pages 77-100 in: Mechanisms of Resistance to Plant Disease. A. Slusarenko, R. S. S. Fraser, and L. C. van Loon, eds. Kluwer Academic Publishers, London. (10.1007/978-94-011-3937-3_4)
{'key': 'p_39', 'first-page': '427', 'volume': '12', 'author': 'Wagner F.', 'year': '1940', 'journal-title': 'Phytopathol. Z.'} / Phytopathol. Z. by Wagner F. (1940)
{'key': 'p_40', 'first-page': '107', 'volume': '8', 'author': 'Yoshida S.', 'year': '1962', 'journal-title': 'Jpn. J. Soil Sci. Plant Nutr.'} / Jpn. J. Soil Sci. Plant Nutr. by Yoshida S. (1962)
10.1139/b93-029
10.1016/S0048-4059(83)81043-1
Zeyen, R. J., Carver, T. L. W., and Lyngkjaer, M. F. 2002. The formation and role of papillae. Pages 107-125 in: The Powdery Mildews: A Comprehensive Treatise. R. R. Bélanger, W. R. Bushnell, A. J. Dik, and T. L. W. Carver, eds. The American Phytopathological Society, St. Paul, MN.

Dates

Type	When
Created	18 years, 3 months ago (May 11, 2007, 7:20 a.m.)
Deposited	3 years, 8 months ago (Dec. 29, 2021, 3:38 p.m.)
Indexed	4 weeks, 1 day ago (Aug. 7, 2025, 4:56 a.m.)
Issued	22 years, 5 months ago (April 1, 2003)
Published	22 years, 5 months ago (April 1, 2003)
Published Print	22 years, 5 months ago (April 1, 2003)

Funders 0

None

BibTeX

@article{B_langer_2003, title={Cytological Evidence of an Active Role of Silicon in Wheat Resistance to Powdery Mildew (Blumeria graminis f. sp. tritici)}, volume={93}, ISSN={1943-7684}, url={http://dx.doi.org/10.1094/phyto.2003.93.4.402}, DOI={10.1094/phyto.2003.93.4.402}, number={4}, journal={Phytopathology®}, publisher={Scientific Societies}, author={Bélanger, R. R. and Benhamou, Nicole and Menzies, J. G.}, year={2003}, month=apr, pages={402–412} }

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