DOI: 10.1094/phyto.1998.88.4.276. Resistance to <i>Aspergillus flavus</i> in Corn Kernels Is Associated with a 14-kDa Protein

Resistance to Aspergillus flavus in Corn Kernels Is Associated with a 14-kDa Protein

10.1094/phyto.1998.88.4.276

Crossref journal-article

Scientific Societies

Phytopathology® (1665)

Abstract

Corn genotypes resistant or susceptible to Aspergillus flavus were extracted for protein analysis using a pH 2.8 buffer. The profile of protein extracts revealed that a 14-kDa protein is present in relatively high concentration in kernels of seven resistant corn genotypes, but is absent or present only in low concentration in kernels of six susceptible ones. The N-terminal sequence of this 14-kDa protein showed 100% homology to a corn trypsin inhibitor. The 14-kDa protein purified from resistant varieties also demonstrated in vitro inhibition of both trypsin activity and the growth of A. flavus. This is the first demonstration of antifungal activity of a corn 14-kDa trypsin inhibitor protein. The expression of this protein among tested genotypes may be related to their difference in resistance to A. flavus infection and subsequent aflatoxin contamination.

Bibliography

Chen, Z.-Y., Brown, R. L., Lax, A. R., Guo, B. Z., Cleveland, T. E., & Russin, J. S. (1998). Resistance to Aspergillus flavus in Corn Kernels Is Associated with a 14-kDa Protein. PhytopathologyÂ®, 88(4), 276â281.

Authors 6

Z.-Y. Chen (first)
R. L. Brown (additional)
A. R. Lax (additional)
B. Z. Guo (additional)
T. E. Cleveland (additional)
J. S. Russin (additional)

References 40 Referenced 101

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Chen, Z.Y., Brown, R. L., Lax, A. R., Guo, B. Z., Cleveland, T. E., and Russin, J. S. 1997. Resistance to Aspergillus flavus in corn kernels is associated with a 14 kDa protein. (Abstr.) Phytopathology 87:S19. (10.1094/PHYTO.1998.88.4.276)
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Chilosi, G., Caruso, C., Caporale, C., Vacca, F., Magro, P., Buonocore, V., Salzman, R., Bordelon, B., Bressan, R., and Hasegawa, M. 1996. Wheat seed PR-4 and trypsin inhibitor have substantial in vitro antifungal activities against Aspergillus flavus. Page 29 in: Proc. Aflatoxin Elimination Workshop. J. Robens and T. E. Cleveland, eds. U.S. Dep. Agric. Agric Res. Serv., Washington, DC.
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Dates

Type	When
Created	18 years, 3 months ago (May 11, 2007, 6:51 a.m.)
Deposited	3 years, 8 months ago (Dec. 29, 2021, 3:10 p.m.)
Indexed	4 days ago (Sept. 2, 2025, 6:39 a.m.)
Issued	27 years, 5 months ago (April 1, 1998)
Published	27 years, 5 months ago (April 1, 1998)
Published Print	27 years, 5 months ago (April 1, 1998)

Funders 0

None

BibTeX

@article{Chen_1998, title={Resistance to Aspergillus flavus in Corn Kernels Is Associated with a 14-kDa Protein}, volume={88}, ISSN={1943-7684}, url={http://dx.doi.org/10.1094/phyto.1998.88.4.276}, DOI={10.1094/phyto.1998.88.4.276}, number={4}, journal={Phytopathology®}, publisher={Scientific Societies}, author={Chen, Z.-Y. and Brown, R. L. and Lax, A. R. and Guo, B. Z. and Cleveland, T. E. and Russin, J. S.}, year={1998}, month=apr, pages={276–281} }

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