DOI: 10.1094/mpmi.2004.17.11.1250. Transcriptional Regulation of Components of the Type III Secretion System and Effectors in <i>Pseudomonas syringae</i> pv. <i>phaseolicola</i>

Transcriptional Regulation of Components of the Type III Secretion System and Effectors in Pseudomonas syringae pv. phaseolicola

10.1094/mpmi.2004.17.11.1250

Crossref journal-article

Scientific Societies

Molecular Plant-Microbe Interactions® (1665)

Abstract

Quantitative real-time polymerase chain reaction was used with specific TaqMan probes to examine transcription of selected hrp and effector genes in Pseudomonas syringae pv. phaseolicola strains 1448A (race 6) and 1449B (race 7). Transcripts examined were from genes encoding the regulators hrpR and hrpL, core structural components of the type III secretion system (TTSS) hrcC, hrcJ, hrcN, hrcU, and hrpA; the first open-reading frame of each hrp operon, including hrpF, hrpJ, hrpP, and hrpY; and also secreted effectors hrpZ, avrPphE, avrPphF, and virPphA. All genes were induced by incubation in a minimal medium and showed patterns of expression indicating regulation by HrpRS and HrpL. Basal mRNA levels and the timing of accumulation of transcripts after induction differed significantly, suggesting the operation of additional regulatory elements. However, no clear transcriptional hierarchy emerged to explain the ordered construction of the TTSS. Quantitative analysis confirmed that the rates and levels of transcript accumulation within the first 2 h after inoculation were considerably higher in planta than in vitro, and indicated that plant cell wall contact may enhance transcription of TTSS and effector genes in P. syringae pv. phaseolicola. The low-abundance hrcU mRNA had a half-life of 16.5 min, whereas other transcripts had half-lives between 3 and 8 min.

Bibliography

Thwaites, R., Spanu, P. D., Panopoulos, N. J., Stevens, C., & Mansfield, J. W. (2004). Transcriptional Regulation of Components of the Type III Secretion System and Effectors in Pseudomonas syringae pv. phaseolicola. Molecular Plant-Microbe InteractionsÂ®, 17(11), 1250â1258.

Authors 5

R. Thwaites (first)
P. D. Spanu (additional)
N. J. Panopoulos (additional)
C. Stevens (additional)
J. W. Mansfield (additional)

References 42 Referenced 37

10.1093/emboj/19.10.2304
10.1073/pnas.112318199
10.1073/pnas.0535335100
10.1046/j.1365-2958.1996.5731077.x
10.1046/j.1365-2958.2002.03008.x
10.1016/0885-5765(88)90003-3
10.1677/jme.0.0290023
10.1016/S0966-842X(02)02451-4
10.1146/annurev.micro.54.1.735
10.1046/j.1365-2958.2001.02235.x
10.1073/pnas.032514099
10.1111/j.1365-2958.1995.tb02230.x
10.1126/science.295.5560.1722
10.1016/0885-5765(87)90061-0
10.1016/S0966-842X(97)01163-3
10.1007/s00438-001-0619-4
10.1126/science.2781284
10.1073/pnas.96.19.10875
10.1126/science.1066397
10.1016/S1286-4579(03)00032-7
10.1016/S0092-8674(00)00053-2
10.1128/jb.172.2.741-747.1990
10.1073/pnas.011265298
10.1093/emboj/21.8.1909
10.1146/annurev.phyto.35.1.129
10.1128/jb.168.2.512-522.1986
10.1094/MPMI-7-0726
10.1046/j.1365-2958.1998.00692.x
10.1094/MPMI.2003.16.3.249
10.1094/MPMI-8-0717
10.1128/JB.180.17.4532-4537.1998
10.1128/JB.174.11.3499-3507.1992
{'key': 'p_33', 'first-page': '330', 'volume': '257', 'author': 'Schirmer F.', 'year': '1998', 'journal-title': 'Mol. Gen. Genet.'} / Mol. Gen. Genet. by Schirmer F. (1998)
10.1101/gr.912603
10.1016/S0168-6445(03)00064-0
10.1016/S0966-842X(03)00155-0
10.1046/j.1365-3059.1999.00321.x
10.1093/emboj/19.13.3204
10.1023/A:1008661415950
10.1128/jb.178.4.1061-1069.1996
10.1128/jb.176.10.3089-3091.1994
10.1046/j.1365-2958.2002.02964.x

Dates

Type	When
Created	18 years, 3 months ago (May 11, 2007, 5:26 a.m.)
Deposited	3 years, 8 months ago (Dec. 29, 2021, 3:01 p.m.)
Indexed	1 week, 1 day ago (Aug. 27, 2025, 11:56 a.m.)
Issued	20 years, 10 months ago (Nov. 1, 2004)
Published	20 years, 10 months ago (Nov. 1, 2004)
Published Print	20 years, 10 months ago (Nov. 1, 2004)

Funders 0

None

BibTeX

@article{Thwaites_2004, title={Transcriptional Regulation of Components of the Type III Secretion System and Effectors in Pseudomonas syringae pv. phaseolicola}, volume={17}, ISSN={1943-7706}, url={http://dx.doi.org/10.1094/mpmi.2004.17.11.1250}, DOI={10.1094/mpmi.2004.17.11.1250}, number={11}, journal={Molecular Plant-Microbe Interactions®}, publisher={Scientific Societies}, author={Thwaites, R. and Spanu, P. D. and Panopoulos, N. J. and Stevens, C. and Mansfield, J. W.}, year={2004}, month=nov, pages={1250–1258} }

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