DOI: 10.1094/mpmi.2001.14.7.877. Characterization of a <i>PR-10</i> Pathogenesis-Related Gene Family Induced in Rice During Infection with <i>Magnaporthe grisea</i>

Characterization of a PR-10 Pathogenesis-Related Gene Family Induced in Rice During Infection with Magnaporthe grisea

10.1094/mpmi.2001.14.7.877

Crossref journal-article

Scientific Societies

Molecular Plant-Microbe Interactions® (1665)

Abstract

A partial cDNA with homology to the PR-10 class of pathogenesis-related proteins was used to screen a rice genomic library. One 16-kb genomic clone contained three genes with PR-10 similarity. These genes, RPR10a, RPR10b, and RPR10c, were arranged in tandem and separated by approximately 2.5 kb. RPR10a cDNA was obtained by reverse transcription-polymerase chain reaction, and sequence analysis revealed that RPR10a and RPR10b encode predicted proteins of 158 and 160 amino acids, respectively, and share 71% amino acid identity. RPR10c appears to be a nonfunctional pseudogene. Gene-specific probes were used to study transcript accumulations of the three RPR10 genes in rice plants following inoculation with Magnaporthe grisea. RPR10a transcripts were induced from a low basal level within 12 h after inoculation and showed a second higher level induction at 48 h, which continued throughout the 144 h it was examined. In addition, RPR10a was induced strongly by salicylic and jasmonic acid applications to rice plants. Transcripts of RPR10b also were enhanced by M. grisea, but were not strongly visible until 48 h after inoculation. Tissue prints of M. grisea-infected rice leaves when the RPR10a-specific probe was used indicate that RPR10a is expressed most strongly in a localized fashion in response to the pathogen.

Bibliography

McGee, J. D., Hamer, J. E., & Hodges, T. K. (2001). Characterization of a PR-10 Pathogenesis-Related Gene Family Induced in Rice During Infection with Magnaporthe grisea. Molecular Plant-Microbe InteractionsÂ®, 14(7), 877â886.

Authors 3

J. David McGee (first)
John E. Hamer (additional)
Thomas K. Hodges (additional)

References 48 Referenced 141

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Dates

Type	When
Created	18 years, 3 months ago (May 11, 2007, 5:17 a.m.)
Deposited	3 years, 8 months ago (Dec. 29, 2021, 2:55 p.m.)
Indexed	4 days, 4 hours ago (Sept. 2, 2025, 6:39 a.m.)
Issued	24 years, 2 months ago (July 1, 2001)
Published	24 years, 2 months ago (July 1, 2001)
Published Print	24 years, 2 months ago (July 1, 2001)

Funders 0

None

BibTeX

@article{McGee_2001, title={Characterization of a PR-10 Pathogenesis-Related Gene Family Induced in Rice During Infection with Magnaporthe grisea}, volume={14}, ISSN={1943-7706}, url={http://dx.doi.org/10.1094/mpmi.2001.14.7.877}, DOI={10.1094/mpmi.2001.14.7.877}, number={7}, journal={Molecular Plant-Microbe Interactions®}, publisher={Scientific Societies}, author={McGee, J. David and Hamer, John E. and Hodges, Thomas K.}, year={2001}, month=jul, pages={877–886} }

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