DOI: 10.1094/mpmi.2001.14.5.629. Genetic Mapping and Functional Analysis of the Tomato Bs4 Locus Governing Recognition of the Xanthomonas campestris pv. vesicatoria AvrBs4 Protein

Genetic Mapping and Functional Analysis of the Tomato Bs4 Locus Governing Recognition of the Xanthomonas campestris pv. vesicatoria AvrBs4 Protein

10.1094/mpmi.2001.14.5.629

Crossref journal-article

Scientific Societies

Molecular Plant-Microbe Interactions® (1665)

Abstract

Xanthomonas campestris pv. vesicatoria is the causal agent of bacterial spot disease on pepper (Capsicum spp.) and tomato (Lycopersicon spp.). Analysis of 17 different Lycopersicon accessions with avrBs4-expressing X. campestris pv. vesicatoria strains identified 15 resistant and two susceptible tomato genotypes. Genetic analysis revealed that AvrBs4 recognition in tomato is governed by a single locus, designated Bs4 (bacterial spot resistance locus no. 4). Amplified fragment length polymorphism and bulked DNA templates from resistant and susceptible plants were used to define a 2.6-cM interval containing the Bs4 locus. A standard tomato mapping population was employed to localize Bs4- linked markers on the short arm of chromosome 5. Investigation of X. campestris pv. vesicatoria hrp mutant strains revealed that AvrBs4 secretion and avirulence activity are hrp dependent. Agrobacterium-based delivery of the avrBs4 gene into tomato triggered a plant response that phenotypically resembled the hypersensitive response induced by avrBs4-expressing X. campestris pv. vesicatoria strains, suggesting symplastic perception of the avirulence protein. Mutations in the avrBs4 C-terminal nuclear localization signals (NLSs) showed that NLSs are dispensable for Bs4-mediated recognition. Our data suggest that tomato Bs4 and pepper Bs3 employ different recognition modes for detection of the highly homologous X. campestris pv. vesicatoria avirulence proteins AvrBs4 and AvrBs3.

Bibliography

Ballvora, A., Pierre, M., van den Ackerveken, G., Schornack, S., Rossier, O., Ganal, M., Lahaye, T., & Bonas, U. (2001). Genetic Mapping and Functional Analysis of the Tomato Bs4 Locus Governing Recognition of the Xanthomonas campestris pv. vesicatoria AvrBs4 Protein. Molecular Plant-Microbe InteractionsÂ®, 14(5), 629â638.

Authors 8

Agim Ballvora (first)
Michéle Pierre (additional)
Guido van den Ackerveken (additional)
Sebastian Schornack (additional)
Ombeline Rossier (additional)
Martin Ganal (additional)
Thomas Lahaye (additional)
Ulla Bonas (additional)

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Dates

Type	When
Created	18 years, 3 months ago (May 11, 2007, 5:17 a.m.)
Deposited	3 years, 8 months ago (Dec. 29, 2021, 2:56 p.m.)
Indexed	1 month, 3 weeks ago (July 9, 2025, 6:58 p.m.)
Issued	24 years, 4 months ago (May 1, 2001)
Published	24 years, 4 months ago (May 1, 2001)
Published Print	24 years, 4 months ago (May 1, 2001)

Funders 0

None

BibTeX

@article{Ballvora_2001, title={Genetic Mapping and Functional Analysis of the Tomato Bs4 Locus Governing Recognition of the Xanthomonas campestris pv. vesicatoria AvrBs4 Protein}, volume={14}, ISSN={1943-7706}, url={http://dx.doi.org/10.1094/mpmi.2001.14.5.629}, DOI={10.1094/mpmi.2001.14.5.629}, number={5}, journal={Molecular Plant-Microbe Interactions®}, publisher={Scientific Societies}, author={Ballvora, Agim and Pierre, Michéle and van den Ackerveken, Guido and Schornack, Sebastian and Rossier, Ombeline and Ganal, Martin and Lahaye, Thomas and Bonas, Ulla}, year={2001}, month=may, pages={629–638} }

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