DOI: 10.1094/mpmi.1998.11.11.1136. Agrobacterium tumefaciens Transformation of the Radiation Hypersensitive Arabidopsis thaliana Mutants uvh1 and rad5

Agrobacterium tumefaciens Transformation of the Radiation Hypersensitive Arabidopsis thaliana Mutants uvh1 and rad5

10.1094/mpmi.1998.11.11.1136

Crossref journal-article

Scientific Societies

Molecular Plant-Microbe Interactions® (1665)

Abstract

The Arabidopsis thaliana mutants uvh1 and rad5, originally identified as radiation hypersensitive, were reported to be deficient in T-DNA integration based on the relative efficiencies of stable transformation and T-DNA transfer. We reassessed these mutants for susceptibility to transformation by Agrobacterium tumefaciens. The mutant rad5 showed a significant reduction in the efficiency of transient as well as stable transformation, compared with its wild-type progenitor. These data indicate that rad5 is blocked at a step in the transformation process prior to T-DNA integration. We additionally found, using both an in vitro root inoculation and an in vivo flower bolt inoculation assay, that the mutant uvh1 is as susceptible to A. tumefaciens-mediated transformation as is its wild-type progenitor, C10.

Bibliography

Nam, J., Mysore, K. S., & Gelvin, S. B. (1998). Agrobacterium tumefaciens Transformation of the Radiation Hypersensitive Arabidopsis thaliana Mutants uvh1 and rad5. Molecular Plant-Microbe InteractionsÂ®, 11(11), 1136â1141.

Authors 3

Jaesung Nam (first)
Kirankumar S. Mysore (additional)
Stanton B. Gelvin (additional)

References 26 Referenced 26

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Dates

Type	When
Created	18 years, 3 months ago (May 11, 2007, 4:59 a.m.)
Deposited	3 years, 8 months ago (Dec. 29, 2021, 2:36 p.m.)
Indexed	1 month ago (Aug. 5, 2025, 9:02 a.m.)
Issued	26 years, 10 months ago (Nov. 1, 1998)
Published	26 years, 10 months ago (Nov. 1, 1998)
Published Print	26 years, 10 months ago (Nov. 1, 1998)

Funders 0

None

BibTeX

@article{Nam_1998, title={Agrobacterium tumefaciens Transformation of the Radiation Hypersensitive Arabidopsis thaliana Mutants uvh1 and rad5}, volume={11}, ISSN={1943-7706}, url={http://dx.doi.org/10.1094/mpmi.1998.11.11.1136}, DOI={10.1094/mpmi.1998.11.11.1136}, number={11}, journal={Molecular Plant-Microbe Interactions®}, publisher={Scientific Societies}, author={Nam, Jaesung and Mysore, Kirankumar S. and Gelvin, Stanton B.}, year={1998}, month=nov, pages={1136–1141} }

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