DOI: 10.1094/mpmi.1997.10.9.1075. G Protein α Subunit Genes Control Growth, Development, and Pathogenicity of <i>Magnaporthe grisea</i>

G Protein α Subunit Genes Control Growth, Development, and Pathogenicity of Magnaporthe grisea

10.1094/mpmi.1997.10.9.1075

Crossref journal-article

Scientific Societies

Molecular Plant-Microbe Interactions® (1665)

Abstract

Three G protein α subunit genes have been cloned and characterized from Magnaporthe grisea: magA is very similar to CPG-2 of Cryphonectria parasitica; magB is virtually identical to CPG-1 of Cryphonectria parasitica, to gna1 of Neurospora crassa, and to fadA of Emericella nidulans; and magC is most similar to gna2 of Neurospora crassa. Homologous recombination resulting in targeted deletion of magA had no effect on vegetative growth, conidiation, or appressorium formation. Deletion of magC reduced conidiation, but did not affect vegetative growth or appressorium formation. However, disruption of magB significantly reduced vegetative growth, conidiation, and appressorium formation. magB¯ transformants, unlike magA¯ and magC¯ transformants, exhibited a reduced ability to infect and colonize susceptible rice leaves. G protein α subunit genes are required for M. grisea mating. magB¯ transformants failed to form perithecia, whereas magA¯ and magC¯ transformants did not produce mature asci. These results suggest that G protein α subunit genes are involved in signal transduction pathways in M. grisea that control vegetative growth, conidiation, conidium attachment, appressorium formation, mating, and pathogenicity.

Bibliography

Liu, S., & Dean, R. A. (1997). G Protein Î± Subunit Genes Control Growth, Development, and Pathogenicity of Magnaporthe grisea. Molecular Plant-Microbe InteractionsÂ®, 10(9), 1075â1086.

Authors 2

Shaohua Liu (first)
Ralph A. Dean (additional)

References 48 Referenced 264

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Dates

Type	When
Created	18 years, 3 months ago (May 11, 2007, 4:55 a.m.)
Deposited	3 years, 8 months ago (Dec. 29, 2021, 2:43 p.m.)
Indexed	1 day, 18 hours ago (Sept. 4, 2025, 9:24 a.m.)
Issued	27 years, 9 months ago (Dec. 1, 1997)
Published	27 years, 9 months ago (Dec. 1, 1997)
Published Print	27 years, 9 months ago (Dec. 1, 1997)

Funders 0

None

BibTeX

@article{Liu_1997, title={G Protein α Subunit Genes Control Growth, Development, and Pathogenicity of Magnaporthe grisea}, volume={10}, ISSN={1943-7706}, url={http://dx.doi.org/10.1094/mpmi.1997.10.9.1075}, DOI={10.1094/mpmi.1997.10.9.1075}, number={9}, journal={Molecular Plant-Microbe Interactions®}, publisher={Scientific Societies}, author={Liu, Shaohua and Dean, Ralph A.}, year={1997}, month=dec, pages={1075–1086} }

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  "abstract": "<jats:p> Three G protein \u03b1 subunit genes have been cloned and characterized from Magnaporthe grisea: magA is very similar to CPG-2 of Cryphonectria parasitica; magB is virtually identical to CPG-1 of Cryphonectria parasitica, to gna1 of Neurospora crassa, and to fadA of Emericella nidulans; and magC is most similar to gna2 of Neurospora crassa. Homologous recombination resulting in targeted deletion of magA had no effect on vegetative growth, conidiation, or appressorium formation. Deletion of magC reduced conidiation, but did not affect vegetative growth or appressorium formation. However, disruption of magB significantly reduced vegetative growth, conidiation, and appressorium formation. magB\u00af transformants, unlike magA\u00af and magC\u00af transformants, exhibited a reduced ability to infect and colonize susceptible rice leaves. G protein \u03b1 subunit genes are required for M. grisea mating. magB\u00af transformants failed to form perithecia, whereas magA\u00af and magC\u00af transformants did not produce mature asci. These results suggest that G protein \u03b1 subunit genes are involved in signal transduction pathways in M. grisea that control vegetative growth, conidiation, conidium attachment, appressorium formation, mating, and pathogenicity. </jats:p>",
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