DOI: 10.1094/mpmi.1997.10.7.938. A <i>Rhizobium leguminosarum</i> Biovar <i>trifolii</i> Locus Not Localized on the Sym Plasmid Hinders Effective Nodulation on Plants of the Pea Cross-Inoculation Group

A Rhizobium leguminosarum Biovar trifolii Locus Not Localized on the Sym Plasmid Hinders Effective Nodulation on Plants of the Pea Cross-Inoculation Group

10.1094/mpmi.1997.10.7.938

Crossref journal-article

Scientific Societies

Molecular Plant-Microbe Interactions® (1665)

Abstract

Introduction of the Sym plasmid pRL1JI into the cured Rhizobium leguminosarum bv. trifolii strain RCR5 resulted in a strain, designated RBL5523, that was expected to nodulate plants of the pea cross-inoculation group. However, effective nodulation occurred only on Vicia sativa plants, not on V. hirsuta or Pisum sativum. After random Tn5 mutagenesis, a derivative of RBL5523 was isolated that effectively nodulated and fixed nitrogen on P. sativum and V. hirsuta. Characterization of the mutant, RBL5787, indicated the cell surface components, extracellular polysaccharides, lipopolysaccharides, and outer membrane proteins, as well as the pattern of Nod metabolites, were indistinguishable from those of the parental strain. To obtain an indication of the function of the mutated locus, the flanking regions were sequenced and used to perform searches in protein and nonredundant nucleotide databases. No significant similarity or homology with any known sequence was detected.

Bibliography

Roest, H. P., Mulders, I. H. M., Spaink, H. P., Wijffelman, C. A., & Lugtenberg, B. J. J. (1997). A Rhizobium leguminosarum Biovar trifolii Locus Not Localized on the Sym Plasmid Hinders Effective Nodulation on Plants of the Pea Cross-Inoculation Group. Molecular Plant-Microbe InteractionsÂ®, 10(7), 938â941.

Authors 5

Henk P. Roest (first)
Ine H. M. Mulders (additional)
Herman P. Spaink (additional)
Carel A. Wijffelman (additional)
Ben J. J. Lugtenberg (additional)

References 21 Referenced 33

10.1016/S0022-2836(05)80360-2
10.1038/276633a0
10.1099/00221287-112-1-135
10.1128/jb.170.8.3782-3785.1988
Gray, J. X., de Maagd, R. A., Rolfe, B. G., Johnston, A. W. B., and Lugtenberg, B. J. J. 1992. The role of the Rhizobium cell surface during symbiosis. Pages 357-376 in: Molecular Signals in Plant-Microbe Communications. D. P. S. Verma, ed. CRC Press Inc., Boca Raton, FL.
10.1104/pp.43.8.1185
10.1038/291351a0
10.1111/j.1365-2672.1979.tb00830.x
10.1073/pnas.87.6.2264
10.1128/jb.173.9.2791-2799.1991
10.1016/0092-8674(89)90893-3
Maniatis, T., Fritsch, E. F., and Sambrook, J. 1982. Molecular Cloning: A Laboratory Manual. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.
10.1016/0378-1119(84)90022-2
10.1111/j.1365-2958.1993.tb01665.x
10.1094/MPMI-7-0099
10.1111/j.1574-6968.1984.tb01466.x
10.1128/jb.177.17.4985-4991.1995
10.1094/MPMI-5-072
10.1099/00221287-101-1-51
{'key': 'p_20', 'first-page': '317', 'volume': '27', 'author': 'van Brussel A. A. N.', 'year': '1982', 'journal-title': 'Lett.'} / Lett. by van Brussel A. A. N. (1982)
10.1104/pp.98.1.143

Dates

Type	When
Created	18 years, 3 months ago (May 11, 2007, 4:56 a.m.)
Deposited	3 years, 7 months ago (Dec. 29, 2021, 2:45 p.m.)
Indexed	1 year ago (Aug. 24, 2024, 7:06 p.m.)
Issued	27 years, 11 months ago (Sept. 1, 1997)
Published	27 years, 11 months ago (Sept. 1, 1997)
Published Print	27 years, 11 months ago (Sept. 1, 1997)

Funders 0

None

BibTeX

@article{Roest_1997, title={A Rhizobium leguminosarum Biovar trifolii Locus Not Localized on the Sym Plasmid Hinders Effective Nodulation on Plants of the Pea Cross-Inoculation Group}, volume={10}, ISSN={1943-7706}, url={http://dx.doi.org/10.1094/mpmi.1997.10.7.938}, DOI={10.1094/mpmi.1997.10.7.938}, number={7}, journal={Molecular Plant-Microbe Interactions®}, publisher={Scientific Societies}, author={Roest, Henk P. and Mulders, Ine H. M. and Spaink, Herman P. and Wijffelman, Carel A. and Lugtenberg, Ben J. J.}, year={1997}, month=sep, pages={938–941} }

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