DOI: 10.1093/nar/gkq1323. Mutant CAG repeats of Huntingtin transcript fold into hairpins, form nuclear foci and are targets for RNA interference

Mutant CAG repeats of Huntingtin transcript fold into hairpins, form nuclear foci and are targets for RNA interference

10.1093/nar/gkq1323

Crossref journal-article

Oxford University Press (OUP)

Nucleic Acids Research (286)

Bibliography

de Mezer, M., Wojciechowska, M., Napierala, M., Sobczak, K., & Krzyzosiak, W. J. (2011). Mutant CAG repeats of Huntingtin transcript fold into hairpins, form nuclear foci and are targets for RNA interference. Nucleic Acids Research, 39(9), 3852â3863.

Authors 5

Mateusz de Mezer (first)
Marzena Wojciechowska (additional)
Marek Napierala (additional)
Krzysztof Sobczak (additional)
Wlodzimierz J. Krzyzosiak (additional)

References 43 Referenced 165

10.1038/nrg1686 / Nat. Rev. Genet. / History of genetic disease: the molecular genetics of Huntington disease - a history by Bates (2005)
10.1056/NEJM198611133152006 / N. Engl. J. Med. / Huntington's disease. Pathogenesis and management by Martin (1986)
10.1016/0092-8674(93)90585-E / Cell / A novel gene containing a trinucleotide repeat that is expanded and unstable on Huntington's disease chromosomes by The Huntington's Disease Collaborative Research Group (1993)
10.1038/352077a0 / Nature / Androgen receptor gene mutations in X-linked spinal and bulbar muscular atrophy by La Spada (1991)
10.1038/nrg1691 / Nat. Rev. Genet. / Diseases of unstable repeat expansion: mechanisms and common principles by Gatchel (2005)
10.1126/science.277.5334.1990 / Science / Aggregation of huntingtin in neuronal intranuclear inclusions and dystrophic neurites in brain by DiFiglia (1997)
{'key': 'key\n\t\t\t\t20170510153524_B7', 'first-page': '338', 'article-title': "Huntington's disease: how does huntingtin, an anti-apoptotic protein, become toxic? Pathol", 'volume': '52', 'author': 'Rangone', 'year': '2004', 'journal-title': 'Biol.'} / Biol. / Huntington's disease: how does huntingtin, an anti-apoptotic protein, become toxic? Pathol by Rangone (2004)
10.1016/S0092-8674(00)81782-1 / Cell / Huntingtin acts in the nucleus to induce apoptosis but death does not correlate with the formation of intranuclear inclusions by Saudou (1998)
10.1093/hmg/10.18.1879 / Hum. Mol. Genet. / The myotonic dystrophy expanded CUG repeat tract is necessary but not sufficient to disrupt C2C12 myoblast differentiation by Amack (2001)
10.1126/science.289.5485.1769 / Science / Myotonic dystrophy in transgenic mice expressing an expanded CUG repeat by Mankodi (2000)
{'key': 'key\n\t\t\t\t20170510153524_B11', 'first-page': '385', 'article-title': 'Molecular basis of myotonic dystrophy: expansion of a trinucleotide (CTG) repeat at the 3′ end of a transcript encoding a protein kinase family member', 'volume': '69', 'author': 'Brook', 'year': '1992', 'journal-title': 'Cell'} / Cell / Molecular basis of myotonic dystrophy: expansion of a trinucleotide (CTG) repeat at the 3′ end of a transcript encoding a protein kinase family member by Brook (1992)
10.1126/science.1062125 / Science / Myotonic dystrophy type 2 caused by a CCTG expansion in intron 1 of ZNF9 by Liquori (2001)
10.1038/emboj.2010.21 / EMBO J. / Sam68 sequestration and partial loss of function are associated with splicing alterations in FXTAS patients by Sellier (2010)
10.1016/j.tins.2004.03.003 / Trends Neurosci. / RNA gains a new function: a mediator of neurodegeneration by Orr (2004)
10.1242/jcs.02404 / J. Cell Sci. / Colocalization of muscleblind with RNA foci is separable from mis-regulation of alternative splicing in myotonic dystrophy by Ho (2005)
10.1038/nature06909 / Nature / RNA toxicity is a component of ataxin-3 degeneration in Drosophila by Li (2008)
10.1074/jbc.272.49.31079 / J. Biol. Chem. / CUG repeats present in myotonin kinase RNA form metastable “slippery” hairpins by Napierala (1997)
10.1016/j.jmb.2004.05.021 / J. Mol. Biol. / Molecular architecture of CAG repeats in human disease related transcripts by Michlewski (2004)
10.1074/jbc.M405130200 / J. Biol. Chem. / Imperfect CAG repeats form diverse structures in SCA1 transcripts by Sobczak (2004)
10.1074/jbc.M409984200 / J. Biol. Chem. / CAG repeats containing CAA interruptions form branched hairpin structures in spinocerebellar ataxia type 2 transcripts by Sobczak (2005)
10.1093/nar/gki186 / Nucleic Acids Res. / Facile FMR1 mRNA structure regulation by interruptions in CGG repeats by Napierala (2005)
10.1006/jmbi.1999.2700 / J. Mol. Biol. / Expanded Sequence Dependence of Thermodynamic Parameters Improves Prediction of RNA Secondary Structure by Mathews (1999)
10.1093/nar/gkg595 / Nucleic Acids Res. / Mfold web server for nucleic acid folding and hybridization prediction by Zuker (2003)
10.1093/nar/gkg766 / Nucleic Acids Res. / RNA structure of trinucleotide repeats associated with human neurological diseases by Sobczak (2003)
10.1093/nar/15.22.9109 / Nucleic Acids Res. / Probing the structure of RNAs in solution by Ehresmann (1987)
10.1016/0076-6879(89)80102-8 / Methods Enzymol. / Enzymatic approaches to probing of RNA secondary and tertiary structure by Knapp (1989)
10.1074/jbc.M109.078790 / J. Biol. Chem. / Structural diversity of triplet repeat RNAs by Sobczak (2010)
10.1016/j.ab.2010.03.021 / Anal. Biochem. / Trinucleotide repeat system for sequence specificity analysis of RNA structure probing reagents by Sobczak (2010)
10.1074/jbc.M404931200 / J. Biol. Chem. / Structural features of microRNA (miRNA) precursors and their relevance to miRNA biogenesis and small interfering RNA/short hairpin RNA design by Krol (2004)
10.1016/0014-5793(89)80641-6 / FEBS Lett. / Structure of the 5′-external transcribed spacer of the human ribosomal RNA gene by Renalier (1989)
10.1002/humu.20466 / Hum. Mutat. / CAG and CTG repeat polymorphism in exons of human genes shows distinct features at the expandable loci by Rozanska (2007)
10.1002/humu.20075 / Hum. Mutat. / Patterns of CAG repeat interruptions in SCA1 and SCA2 genes in relation to repeat instability by Sobczak (2004)
10.1371/journal.pone.0003968 / PLoS One / Cytoplasmic CUG RNA foci are insufficient to elicit key DM1 features by Dansithong (2008)
10.1038/nrm2085 / Nat. Rev. Mol. Cell Biol. / Illuminating the silence: understanding the structure and function of small RNAs by Rana (2007)
10.1016/j.jmb.2005.03.011 / J. Mol. Biol. / Local RNA target structure influences siRNA efficacy: systematic analysis of intentionally designed binding regions by Schubert (2005)
10.1038/ng2135 / Nat. Genet. / The role of site accessibility in microRNA target recognition by Kertesz (2007)
10.1016/j.cell.2007.04.037 / Cell / Molecular basis for target RNA recognition and cleavage by human RISC by Ameres (2007)
10.1261/rna.546207 / RNA / Effect of target secondary structure on RNAi efficiency by Shao (2007)
10.1038/nbt1404 / Nat. Biotechnol. / The impact of target site accessibility on the design of effective siRNAs by Tafer (2008)
10.1093/nar/gkm920 / Nucleic Acids Res. / Efficient siRNA selection using hybridization thermodynamics by Lu (2008)
10.1073/pnas.1231012100 / Proc. Natl Acad. Sci. USA / Allele-specific silencing of dominant disease genes by Miller (2003)
10.1038/nbt.1539 / Nat. Biotechnol. / Allele-specific silencing of mutant huntingtin and ataxin-3 genes by targeting expanded CAG repeats in mRNAs by Hu (2009)
10.1093/nar/gkq700 / Nucleic Acids Res. / Atomic resolution structure of CAG RNA repeats: structural insights and implications for the trinucleotide repeat expansion diseases by Kiliszek (2010)

Dates

Type	When
Created	14 years, 7 months ago (Jan. 19, 2011, 8:14 p.m.)
Deposited	7 years, 4 months ago (April 2, 2018, 10:41 a.m.)
Indexed	1 day, 21 hours ago (Aug. 21, 2025, 1:28 p.m.)
Issued	14 years, 7 months ago (Jan. 18, 2011)
Published	14 years, 7 months ago (Jan. 18, 2011)
Published Online	14 years, 7 months ago (Jan. 18, 2011)
Published Print	14 years, 3 months ago (May 1, 2011)

Funders 0

None

BibTeX

@article{de_Mezer_2011, title={Mutant CAG repeats of Huntingtin transcript fold into hairpins, form nuclear foci and are targets for RNA interference}, volume={39}, ISSN={0305-1048}, url={http://dx.doi.org/10.1093/nar/gkq1323}, DOI={10.1093/nar/gkq1323}, number={9}, journal={Nucleic Acids Research}, publisher={Oxford University Press (OUP)}, author={de Mezer, Mateusz and Wojciechowska, Marzena and Napierala, Marek and Sobczak, Krzysztof and Krzyzosiak, Wlodzimierz J.}, year={2011}, month=jan, pages={3852–3863} }

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      "author": "Sobczak",
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      "author": "Schubert",
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      "author": "Kertesz",
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      "author": "Shao",
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      "author": "Tafer",
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      "author": "Miller",
      "year": "2003",
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      "volume": "27",
      "author": "Hu",
      "year": "2009",
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