DOI: 10.1093/nar/gkl685. Purification of the yeast Slx5–Slx8 protein complex and characterization of its DNA-binding activity

Purification of the yeast Slx5–Slx8 protein complex and characterization of its DNA-binding activity

10.1093/nar/gkl685

Crossref journal-article

Oxford University Press (OUP)

Nucleic Acids Research (286)

Bibliography

Yang, L., Mullen, J. R., & Brill, S. J. (2006). Purification of the yeast Slx5âSlx8 protein complex and characterization of its DNA-binding activity. Nucleic Acids Research, 34(19), 5541â5551.

Authors 3

Litao Yang (first)
Janet R. Mullen (additional)
Steven J. Brill (additional)

References 47 Referenced 35

10.1042/BST0331456 / Biochem. Soc. Trans / Roles of the Bloom's syndrome helicase in the maintenance of genome stability by Cheok (2005)
10.1093/genetics/144.3.935 / Genetics / SGS1, a homologue of the Bloom's and Werner's syndrome genes, is required for maintenance of genome stability in Saccharomyces cerevisiae by Watt (1996)
10.1128/MCB.14.12.8391 / Mol. Cell. Biol / The yeast type I topoisomerase Top3 interacts with Sgs1, a DNA helicase homolog: a potential eukaryotic reverse gyrase by Gangloff (1994)
10.1002/(SICI)1521-1878(199904)21:4<286::AID-BIES4>3.0.CO;2-Z / Bioessays / Defending genome integrity during DNA replication: a proposed role for RecQ family helicases by Chakraverty (1999)
10.1093/genetics/154.3.1101 / Genetics / Bipartite structure of the SGS1 DNA helicase in Saccharomyces cerevisiae by Mullen (2000)
10.1074/jbc.275.13.9636 / J. Biol. Chem / The Bloom's syndrome gene product interacts with topoisomerase III by Wu (2000)
10.1016/S0021-9258(19)61459-6 / J. Biol. Chem / Interaction between yeast Sgs1 helicase and DNA topoisomerase III by Bennett (2000)
10.1074/jbc.M009719200 / J. Biol. Chem / Mapping the DNA topoisomerase III binding domain of the Sgs1 DNA helicase by Fricke (2001)
10.1038/sj.emboj.7600622 / EMBO J / BLAP75, an essential component of Bloom's syndrome protein complexes that maintain genome integrity by Yin (2005)
10.1038/sj.emboj.7600684 / EMBO J / RMI1/NCE4, a suppressor of genome instability, encodes a member of the RecQ helicase/Topo III complex by Chang (2005)
10.1128/MCB.25.11.4476-4487.2005 / Mol. Cell. Biol / Yeast Rmi1/Nce4 controls genome stability as a subunit of the Sgs1–Top3 complex by Mullen (2005)
10.1073/pnas.0508295103 / Proc. Natl Acad. Sci. USA / BLAP75/RMI1 promotes the BLM-dependent dissolution of homologous recombination intermediates by Wu (2006)
10.1016/S1097-2765(00)80354-8 / Mol. Cell / RecQ helicase and topoisomerase III comprise a novel DNA strand passage function: a conserved mechanism for control of DNA recombination by Harmon (1999)
10.1101/gad.12.8.1134 / Genes Dev / RecQ helicase, in concert with RecA and SSB proteins, initiates and disrupts DNA recombination by Harmon (1998)
10.1038/nature02253 / Nature / The Bloom's syndrome helicase suppresses crossing over during homologous recombination by Wu (2003)
10.1016/S0092-8674(03)00886-9 / Cell / Srs2 and Sgs1-Top3 suppress crossovers during double-strand break repair in yeast by Ira (2003)
10.1126/science.1065810 / Science / Systematic genetic analysis with ordered arrays of yeast deletion mutants by Tong (2001)
10.1038/ng1258 / Nature Genet / DNA helicase gene interaction network defined using synthetic lethality analyzed by microarray by Ooi (2003)
10.1093/genetics/157.1.103 / Genetics / Requirement for three novel protein complexes in the absence of the Sgs1 DNA helicase in Saccharomyces cerevisiae by Mullen (2001)
10.1534/genetics.105.052811 / Genetics / Genetic analysis connects SLX5 and SLX8 to the SUMO pathway in Saccharomyces cerevisiae by Wang (2006)
10.1128/MCB.2.8.985 / Mol. Cell. Biol / Sensitivity to the Yeast Plasmid 2u DNA is conferred by the nuclear allele nib1 by Holm (1982)
10.1073/pnas.0500537102 / Proc. Natl Acad. Sci. USA / A SUMO ligase is part of a nuclear multiprotein complex that affects DNA repair and chromosomal organization by Zhao (2005)
10.1128/MCB.25.10.4311-4320.2005 / Mol. Cell. Biol / Misregulation of 2 micron circle copy number in a SUMO pathway mutant by Chen (2005)
10.1128/MCB.25.10.4299-4310.2005 / Mol. Cell. Biol / The 2 micron plasmid causes cell death in Saccharomyces cerevisiae with a mutation in Ulp1 protease by Dobson (2005)
10.1016/j.dnarep.2005.10.010 / DNA Repair (Amst) / Suppression of genomic instability by SLX5 and SLX8 in Saccharomyces cerevisiae by Zhang (2006)
10.1038/76055 / Nature Genet / Homologous recombination is responsible for cell death in the absence of the Sgs1 and Srs2 helicases by Gangloff (2000)
10.1093/emboj/18.6.1701 / EMBO J / The essential role of yeast topoisomerase III in meiosis depends on recombination by Gangloff (1999)
10.1073/pnas.252652399 / Proc. Natl Acad. Sci. USA / Alternate pathways involving Sgs1/Top3, Mus81/Mms4, and Srs2 prevent formation of toxic recombination intermediates from single-stranded gaps created by DNA replication by Fabre (2002)
10.1128/MCB.23.10.3487-3496.2003 / Mol. Cell. Biol / The mechanism of Mus81-Mms4 cleavage site selection distinguishes it from the homologous endonuclease Rad1-Rad10 by Bastin-Shanower (2003)
10.1146/annurev.biochem.67.1.425 / Annu. Rev. Biochem / The ubiquitin system by Hershko (1998)
10.1016/S1097-2765(01)00349-5 / Mol. Cell / Involvement of PIAS1 in the sumoylation of tumor suppressor p53 by Kahyo (2001)
10.1016/S0092-8674(01)00491-3 / Cell / An E3-like factor that promotes SUMO conjugation to the yeast septins by Johnson (2001)
10.1016/S0378-1119(01)00662-X / Gene / A novel factor required for the SUMO1/Smt3 conjugation of yeast septins by Takahashi (2001)
10.1016/S0092-8674(01)00519-0 / Cell / SP-RING for SUMO: new functions bloom for a ubiquitin-like protein by Hochstrasser (2001)
10.1016/0092-8674(89)90584-9 / Cell / Elevated recombination rates in transcriptionally active DNA by Thomas (1989)
{'key': 'key\n\t\t\t\t20170408045537_B36', 'volume-title': 'Methods in Yeast Genetics', 'author': 'Rose', 'year': '1990'} / Methods in Yeast Genetics by Rose (1990)
10.1093/nar/24.13.2519 / Nucleic Acids Res / A new efficient gene disruption cassette for repeated use in budding yeast by Guldener (1996)
10.1016/0076-6879(90)85008-C / Methods Enzymol / Use of T7 RNA polymerase to direct expression of cloned genes by Studier (1990)
{'key': 'key\n\t\t\t\t20170408045537_B39', 'volume-title': 'Methods in Yeast Genetics', 'author': 'Adams', 'year': '1977'} / Methods in Yeast Genetics by Adams (1977)
10.1101/gad.11.24.3375 / Genes Dev / Persistent initiation of DNA replication and chromatin-bound MCM proteins during the cell cycle in cdc6 mutants by Liang (1997)
{'key': 'key\n\t\t\t\t20170408045537_B41', 'first-page': '346', 'article-title': 'Determination of molecular weights and frictional ratios of proteins in impure systems by use of gel filtration and density gradient centrifugation', 'volume': '112', 'author': 'Siegel', 'year': '1966', 'journal-title': 'Biochem. Biophys. Acta'} / Biochem. Biophys. Acta / Determination of molecular weights and frictional ratios of proteins in impure systems by use of gel filtration and density gradient centrifugation by Siegel (1966)
10.1101/gad.932201 / Genes Dev / Functional overlap between Sgs1-Top3 and the Mms4-Mus81 endonuclease by Kaliraman (2001)
10.1101/gad.1105203 / Genes Dev / Slx1-Slx4 is a second structure-specific endonuclease functionally redundant with Sgs1-Top3 by Fricke (2003)
10.1126/science.2511628 / Science / Similarity between the transcriptional silencer binding proteins ABF1 and RAP1 by Diffley (1989)
10.1128/MCB.17.12.7159 / Mol. Cell. Biol / Architecture of the yeast origin recognition complex bound to origins of DNA replication by Lee (1997)
10.1038/35001009 / Nature / A comprehensive analysis of protein–protein interactions in Saccharomyces cerevisiae by Uetz (2000)
10.1016/S0021-9258(19)78100-9 / J. Biol. Chem / Recombinant replication protein A: expression, complex formation, and functional characterization by Henricksen (1994)

Dates

Type	When
Created	18 years, 10 months ago (Oct. 4, 2006, 8:36 p.m.)
Deposited	4 years, 1 month ago (Aug. 1, 2021, 4:49 p.m.)
Indexed	1 year, 1 month ago (July 22, 2024, 8:31 a.m.)
Issued	18 years, 10 months ago (Oct. 4, 2006)
Published	18 years, 10 months ago (Oct. 4, 2006)
Published Online	18 years, 10 months ago (Oct. 4, 2006)
Published Print	18 years, 10 months ago (Nov. 1, 2006)

Funders 0

None

BibTeX

@article{Yang_2006, title={Purification of the yeast Slx5–Slx8 protein complex and characterization of its DNA-binding activity}, volume={34}, ISSN={1362-4962}, url={http://dx.doi.org/10.1093/nar/gkl685}, DOI={10.1093/nar/gkl685}, number={19}, journal={Nucleic Acids Research}, publisher={Oxford University Press (OUP)}, author={Yang, Litao and Mullen, Janet R. and Brill, Steven J.}, year={2006}, month=oct, pages={5541–5551} }

JSON

{
  "indexed": {
    "date-parts": [
      [
        2024,
        7,
        22
      ]
    ],
    "date-time": "2024-07-22T12:31:32Z",
    "timestamp": 1721651492463
  },
  "reference-count": 47,
  "publisher": "Oxford University Press (OUP)",
  "issue": "19",
  "content-domain": {
    "domain": [],
    "crossmark-restriction": false
  },
  "published-print": {
    "date-parts": [
      [
        2006,
        11
      ]
    ]
  },
  "DOI": "10.1093/nar/gkl685",
  "type": "journal-article",
  "created": {
    "date-parts": [
      [
        2006,
        10,
        5
      ]
    ],
    "date-time": "2006-10-05T00:36:13Z",
    "timestamp": 1160008573000
  },
  "page": "5541-5551",
  "source": "Crossref",
  "is-referenced-by-count": 35,
  "title": "Purification of the yeast Slx5\u2013Slx8 protein complex and characterization of its DNA-binding activity",
  "prefix": "10.1093",
  "volume": "34",
  "author": [
    {
      "given": "Litao",
      "family": "Yang",
      "sequence": "first",
      "affiliation": []
    },
    {
      "given": "Janet R.",
      "family": "Mullen",
      "sequence": "additional",
      "affiliation": []
    },
    {
      "given": "Steven J.",
      "family": "Brill",
      "sequence": "additional",
      "affiliation": []
    }
  ],
  "member": "286",
  "published-online": {
    "date-parts": [
      [
        2006,
        10,
        4
      ]
    ]
  },
  "reference": [
    {
      "key": "key\n\t\t\t\t20170408045537_B1",
      "doi-asserted-by": "crossref",
      "first-page": "1456",
      "DOI": "10.1042/BST0331456",
      "article-title": "Roles of the Bloom's syndrome helicase in the maintenance of genome stability",
      "volume": "33",
      "author": "Cheok",
      "year": "2005",
      "journal-title": "Biochem. Soc. Trans"
    },
    {
      "key": "key\n\t\t\t\t20170408045537_B2",
      "doi-asserted-by": "crossref",
      "first-page": "935",
      "DOI": "10.1093/genetics/144.3.935",
      "article-title": "SGS1, a homologue of the Bloom's and Werner's syndrome genes, is required for maintenance of genome stability in Saccharomyces cerevisiae",
      "volume": "144",
      "author": "Watt",
      "year": "1996",
      "journal-title": "Genetics"
    },
    {
      "key": "key\n\t\t\t\t20170408045537_B3",
      "doi-asserted-by": "crossref",
      "first-page": "8391",
      "DOI": "10.1128/MCB.14.12.8391",
      "article-title": "The yeast type I topoisomerase Top3 interacts with Sgs1, a DNA helicase homolog: a potential eukaryotic reverse gyrase",
      "volume": "14",
      "author": "Gangloff",
      "year": "1994",
      "journal-title": "Mol. Cell. Biol"
    },
    {
      "key": "key\n\t\t\t\t20170408045537_B4",
      "doi-asserted-by": "crossref",
      "first-page": "286",
      "DOI": "10.1002/(SICI)1521-1878(199904)21:4<286::AID-BIES4>3.0.CO;2-Z",
      "article-title": "Defending genome integrity during DNA replication: a proposed role for RecQ family helicases",
      "volume": "21",
      "author": "Chakraverty",
      "year": "1999",
      "journal-title": "Bioessays"
    },
    {
      "key": "key\n\t\t\t\t20170408045537_B5",
      "doi-asserted-by": "crossref",
      "first-page": "1101",
      "DOI": "10.1093/genetics/154.3.1101",
      "article-title": "Bipartite structure of the SGS1 DNA helicase in Saccharomyces cerevisiae",
      "volume": "154",
      "author": "Mullen",
      "year": "2000",
      "journal-title": "Genetics"
    },
    {
      "key": "key\n\t\t\t\t20170408045537_B6",
      "doi-asserted-by": "crossref",
      "first-page": "9636",
      "DOI": "10.1074/jbc.275.13.9636",
      "article-title": "The Bloom's syndrome gene product interacts with topoisomerase III",
      "volume": "275",
      "author": "Wu",
      "year": "2000",
      "journal-title": "J. Biol. Chem"
    },
    {
      "key": "key\n\t\t\t\t20170408045537_B7",
      "doi-asserted-by": "crossref",
      "first-page": "26898",
      "DOI": "10.1016/S0021-9258(19)61459-6",
      "article-title": "Interaction between yeast Sgs1 helicase and DNA topoisomerase III",
      "volume": "275",
      "author": "Bennett",
      "year": "2000",
      "journal-title": "J. Biol. Chem"
    },
    {
      "key": "key\n\t\t\t\t20170408045537_B8",
      "doi-asserted-by": "crossref",
      "first-page": "8848",
      "DOI": "10.1074/jbc.M009719200",
      "article-title": "Mapping the DNA topoisomerase III binding domain of the Sgs1 DNA helicase",
      "volume": "276",
      "author": "Fricke",
      "year": "2001",
      "journal-title": "J. Biol. Chem"
    },
    {
      "key": "key\n\t\t\t\t20170408045537_B9",
      "doi-asserted-by": "crossref",
      "first-page": "1465",
      "DOI": "10.1038/sj.emboj.7600622",
      "article-title": "BLAP75, an essential component of Bloom's syndrome protein complexes that maintain genome integrity",
      "volume": "24",
      "author": "Yin",
      "year": "2005",
      "journal-title": "EMBO J"
    },
    {
      "key": "key\n\t\t\t\t20170408045537_B10",
      "doi-asserted-by": "crossref",
      "first-page": "2024",
      "DOI": "10.1038/sj.emboj.7600684",
      "article-title": "RMI1/NCE4, a suppressor of genome instability, encodes a member of the RecQ helicase/Topo III complex",
      "volume": "24",
      "author": "Chang",
      "year": "2005",
      "journal-title": "EMBO J"
    },
    {
      "key": "key\n\t\t\t\t20170408045537_B11",
      "doi-asserted-by": "crossref",
      "first-page": "4476",
      "DOI": "10.1128/MCB.25.11.4476-4487.2005",
      "article-title": "Yeast Rmi1/Nce4 controls genome stability as a subunit of the Sgs1\u2013Top3 complex",
      "volume": "25",
      "author": "Mullen",
      "year": "2005",
      "journal-title": "Mol. Cell. Biol"
    },
    {
      "key": "key\n\t\t\t\t20170408045537_B12",
      "doi-asserted-by": "crossref",
      "first-page": "4068",
      "DOI": "10.1073/pnas.0508295103",
      "article-title": "BLAP75/RMI1 promotes the BLM-dependent dissolution of homologous recombination intermediates",
      "volume": "103",
      "author": "Wu",
      "year": "2006",
      "journal-title": "Proc. Natl Acad. Sci. USA"
    },
    {
      "key": "key\n\t\t\t\t20170408045537_B13",
      "doi-asserted-by": "crossref",
      "first-page": "611",
      "DOI": "10.1016/S1097-2765(00)80354-8",
      "article-title": "RecQ helicase and topoisomerase III comprise a novel DNA strand passage function: a conserved mechanism for control of DNA recombination",
      "volume": "3",
      "author": "Harmon",
      "year": "1999",
      "journal-title": "Mol. Cell"
    },
    {
      "key": "key\n\t\t\t\t20170408045537_B14",
      "doi-asserted-by": "crossref",
      "first-page": "1134",
      "DOI": "10.1101/gad.12.8.1134",
      "article-title": "RecQ helicase, in concert with RecA and SSB proteins, initiates and disrupts DNA recombination",
      "volume": "12",
      "author": "Harmon",
      "year": "1998",
      "journal-title": "Genes Dev"
    },
    {
      "key": "key\n\t\t\t\t20170408045537_B15",
      "doi-asserted-by": "crossref",
      "first-page": "870",
      "DOI": "10.1038/nature02253",
      "article-title": "The Bloom's syndrome helicase suppresses crossing over during homologous recombination",
      "volume": "426",
      "author": "Wu",
      "year": "2003",
      "journal-title": "Nature"
    },
    {
      "key": "key\n\t\t\t\t20170408045537_B16",
      "doi-asserted-by": "crossref",
      "first-page": "401",
      "DOI": "10.1016/S0092-8674(03)00886-9",
      "article-title": "Srs2 and Sgs1-Top3 suppress crossovers during double-strand break repair in yeast",
      "volume": "115",
      "author": "Ira",
      "year": "2003",
      "journal-title": "Cell"
    },
    {
      "key": "key\n\t\t\t\t20170408045537_B17",
      "doi-asserted-by": "crossref",
      "first-page": "2364",
      "DOI": "10.1126/science.1065810",
      "article-title": "Systematic genetic analysis with ordered arrays of yeast deletion mutants",
      "volume": "294",
      "author": "Tong",
      "year": "2001",
      "journal-title": "Science"
    },
    {
      "key": "key\n\t\t\t\t20170408045537_B18",
      "doi-asserted-by": "crossref",
      "first-page": "277",
      "DOI": "10.1038/ng1258",
      "article-title": "DNA helicase gene interaction network defined using synthetic lethality analyzed by microarray",
      "volume": "35",
      "author": "Ooi",
      "year": "2003",
      "journal-title": "Nature Genet"
    },
    {
      "key": "key\n\t\t\t\t20170408045537_B19",
      "doi-asserted-by": "crossref",
      "first-page": "103",
      "DOI": "10.1093/genetics/157.1.103",
      "article-title": "Requirement for three novel protein complexes in the absence of the Sgs1 DNA helicase in Saccharomyces cerevisiae",
      "volume": "157",
      "author": "Mullen",
      "year": "2001",
      "journal-title": "Genetics"
    },
    {
      "key": "key\n\t\t\t\t20170408045537_B20",
      "doi-asserted-by": "crossref",
      "first-page": "1499",
      "DOI": "10.1534/genetics.105.052811",
      "article-title": "Genetic analysis connects SLX5 and SLX8 to the SUMO pathway in Saccharomyces cerevisiae",
      "volume": "172",
      "author": "Wang",
      "year": "2006",
      "journal-title": "Genetics"
    },
    {
      "key": "key\n\t\t\t\t20170408045537_B21",
      "doi-asserted-by": "crossref",
      "first-page": "985",
      "DOI": "10.1128/MCB.2.8.985",
      "article-title": "Sensitivity to the Yeast Plasmid 2u DNA is conferred by the nuclear allele nib1",
      "volume": "2",
      "author": "Holm",
      "year": "1982",
      "journal-title": "Mol. Cell. Biol"
    },
    {
      "key": "key\n\t\t\t\t20170408045537_B22",
      "doi-asserted-by": "crossref",
      "first-page": "4777",
      "DOI": "10.1073/pnas.0500537102",
      "article-title": "A SUMO ligase is part of a nuclear multiprotein complex that affects DNA repair and chromosomal organization",
      "volume": "102",
      "author": "Zhao",
      "year": "2005",
      "journal-title": "Proc. Natl Acad. Sci. USA"
    },
    {
      "key": "key\n\t\t\t\t20170408045537_B23",
      "doi-asserted-by": "crossref",
      "first-page": "4311",
      "DOI": "10.1128/MCB.25.10.4311-4320.2005",
      "article-title": "Misregulation of 2 micron circle copy number in a SUMO pathway mutant",
      "volume": "25",
      "author": "Chen",
      "year": "2005",
      "journal-title": "Mol. Cell. Biol"
    },
    {
      "key": "key\n\t\t\t\t20170408045537_B24",
      "doi-asserted-by": "crossref",
      "first-page": "4299",
      "DOI": "10.1128/MCB.25.10.4299-4310.2005",
      "article-title": "The 2 micron plasmid causes cell death in Saccharomyces cerevisiae with a mutation in Ulp1 protease",
      "volume": "25",
      "author": "Dobson",
      "year": "2005",
      "journal-title": "Mol. Cell. Biol"
    },
    {
      "key": "key\n\t\t\t\t20170408045537_B25",
      "doi-asserted-by": "crossref",
      "first-page": "336",
      "DOI": "10.1016/j.dnarep.2005.10.010",
      "article-title": "Suppression of genomic instability by SLX5 and SLX8 in Saccharomyces cerevisiae",
      "volume": "5",
      "author": "Zhang",
      "year": "2006",
      "journal-title": "DNA Repair (Amst)"
    },
    {
      "key": "key\n\t\t\t\t20170408045537_B26",
      "doi-asserted-by": "crossref",
      "first-page": "192",
      "DOI": "10.1038/76055",
      "article-title": "Homologous recombination is responsible for cell death in the absence of the Sgs1 and Srs2 helicases",
      "volume": "25",
      "author": "Gangloff",
      "year": "2000",
      "journal-title": "Nature Genet"
    },
    {
      "key": "key\n\t\t\t\t20170408045537_B27",
      "doi-asserted-by": "crossref",
      "first-page": "1701",
      "DOI": "10.1093/emboj/18.6.1701",
      "article-title": "The essential role of yeast topoisomerase III in meiosis depends on recombination",
      "volume": "18",
      "author": "Gangloff",
      "year": "1999",
      "journal-title": "EMBO J"
    },
    {
      "key": "key\n\t\t\t\t20170408045537_B28",
      "doi-asserted-by": "crossref",
      "first-page": "16887",
      "DOI": "10.1073/pnas.252652399",
      "article-title": "Alternate pathways involving Sgs1/Top3, Mus81/Mms4, and Srs2 prevent formation of toxic recombination intermediates from single-stranded gaps created by DNA replication",
      "volume": "99",
      "author": "Fabre",
      "year": "2002",
      "journal-title": "Proc. Natl Acad. Sci. USA"
    },
    {
      "key": "key\n\t\t\t\t20170408045537_B29",
      "doi-asserted-by": "crossref",
      "first-page": "3487",
      "DOI": "10.1128/MCB.23.10.3487-3496.2003",
      "article-title": "The mechanism of Mus81-Mms4 cleavage site selection distinguishes it from the homologous endonuclease Rad1-Rad10",
      "volume": "23",
      "author": "Bastin-Shanower",
      "year": "2003",
      "journal-title": "Mol. Cell. Biol"
    },
    {
      "key": "key\n\t\t\t\t20170408045537_B30",
      "doi-asserted-by": "crossref",
      "first-page": "425",
      "DOI": "10.1146/annurev.biochem.67.1.425",
      "article-title": "The ubiquitin system",
      "volume": "67",
      "author": "Hershko",
      "year": "1998",
      "journal-title": "Annu. Rev. Biochem"
    },
    {
      "key": "key\n\t\t\t\t20170408045537_B31",
      "doi-asserted-by": "crossref",
      "first-page": "713",
      "DOI": "10.1016/S1097-2765(01)00349-5",
      "article-title": "Involvement of PIAS1 in the sumoylation of tumor suppressor p53",
      "volume": "8",
      "author": "Kahyo",
      "year": "2001",
      "journal-title": "Mol. Cell"
    },
    {
      "key": "key\n\t\t\t\t20170408045537_B32",
      "doi-asserted-by": "crossref",
      "first-page": "735",
      "DOI": "10.1016/S0092-8674(01)00491-3",
      "article-title": "An E3-like factor that promotes SUMO conjugation to the yeast septins",
      "volume": "106",
      "author": "Johnson",
      "year": "2001",
      "journal-title": "Cell"
    },
    {
      "key": "key\n\t\t\t\t20170408045537_B33",
      "doi-asserted-by": "crossref",
      "first-page": "223",
      "DOI": "10.1016/S0378-1119(01)00662-X",
      "article-title": "A novel factor required for the SUMO1/Smt3 conjugation of yeast septins",
      "volume": "275",
      "author": "Takahashi",
      "year": "2001",
      "journal-title": "Gene"
    },
    {
      "key": "key\n\t\t\t\t20170408045537_B34",
      "doi-asserted-by": "crossref",
      "first-page": "5",
      "DOI": "10.1016/S0092-8674(01)00519-0",
      "article-title": "SP-RING for SUMO: new functions bloom for a ubiquitin-like protein",
      "volume": "107",
      "author": "Hochstrasser",
      "year": "2001",
      "journal-title": "Cell"
    },
    {
      "key": "key\n\t\t\t\t20170408045537_B35",
      "doi-asserted-by": "crossref",
      "first-page": "619",
      "DOI": "10.1016/0092-8674(89)90584-9",
      "article-title": "Elevated recombination rates in transcriptionally active DNA",
      "volume": "56",
      "author": "Thomas",
      "year": "1989",
      "journal-title": "Cell"
    },
    {
      "key": "key\n\t\t\t\t20170408045537_B36",
      "volume-title": "Methods in Yeast Genetics",
      "author": "Rose",
      "year": "1990"
    },
    {
      "key": "key\n\t\t\t\t20170408045537_B37",
      "doi-asserted-by": "crossref",
      "first-page": "2519",
      "DOI": "10.1093/nar/24.13.2519",
      "article-title": "A new efficient gene disruption cassette for repeated use in budding yeast",
      "volume": "24",
      "author": "Guldener",
      "year": "1996",
      "journal-title": "Nucleic Acids Res"
    },
    {
      "key": "key\n\t\t\t\t20170408045537_B38",
      "doi-asserted-by": "crossref",
      "first-page": "60",
      "DOI": "10.1016/0076-6879(90)85008-C",
      "article-title": "Use of T7 RNA polymerase to direct expression of cloned genes",
      "volume": "185",
      "author": "Studier",
      "year": "1990",
      "journal-title": "Methods Enzymol"
    },
    {
      "key": "key\n\t\t\t\t20170408045537_B39",
      "volume-title": "Methods in Yeast Genetics",
      "author": "Adams",
      "year": "1977"
    },
    {
      "key": "key\n\t\t\t\t20170408045537_B40",
      "doi-asserted-by": "crossref",
      "first-page": "3375",
      "DOI": "10.1101/gad.11.24.3375",
      "article-title": "Persistent initiation of DNA replication and chromatin-bound MCM proteins during the cell cycle in cdc6 mutants",
      "volume": "11",
      "author": "Liang",
      "year": "1997",
      "journal-title": "Genes Dev"
    },
    {
      "key": "key\n\t\t\t\t20170408045537_B41",
      "first-page": "346",
      "article-title": "Determination of molecular weights and frictional ratios of proteins in impure systems by use of gel filtration and density gradient centrifugation",
      "volume": "112",
      "author": "Siegel",
      "year": "1966",
      "journal-title": "Biochem. Biophys. Acta"
    },
    {
      "key": "key\n\t\t\t\t20170408045537_B42",
      "doi-asserted-by": "crossref",
      "first-page": "2730",
      "DOI": "10.1101/gad.932201",
      "article-title": "Functional overlap between Sgs1-Top3 and the Mms4-Mus81 endonuclease",
      "volume": "15",
      "author": "Kaliraman",
      "year": "2001",
      "journal-title": "Genes Dev"
    },
    {
      "key": "key\n\t\t\t\t20170408045537_B43",
      "doi-asserted-by": "crossref",
      "first-page": "1768",
      "DOI": "10.1101/gad.1105203",
      "article-title": "Slx1-Slx4 is a second structure-specific endonuclease functionally redundant with Sgs1-Top3",
      "volume": "17",
      "author": "Fricke",
      "year": "2003",
      "journal-title": "Genes Dev"
    },
    {
      "key": "key\n\t\t\t\t20170408045537_B44",
      "doi-asserted-by": "crossref",
      "first-page": "1034",
      "DOI": "10.1126/science.2511628",
      "article-title": "Similarity between the transcriptional silencer binding proteins ABF1 and RAP1",
      "volume": "246",
      "author": "Diffley",
      "year": "1989",
      "journal-title": "Science"
    },
    {
      "key": "key\n\t\t\t\t20170408045537_B45",
      "doi-asserted-by": "crossref",
      "first-page": "7159",
      "DOI": "10.1128/MCB.17.12.7159",
      "article-title": "Architecture of the yeast origin recognition complex bound to origins of DNA replication",
      "volume": "17",
      "author": "Lee",
      "year": "1997",
      "journal-title": "Mol. Cell. Biol"
    },
    {
      "key": "key\n\t\t\t\t20170408045537_B46",
      "doi-asserted-by": "crossref",
      "first-page": "623",
      "DOI": "10.1038/35001009",
      "article-title": "A comprehensive analysis of protein\u2013protein interactions in Saccharomyces cerevisiae",
      "volume": "403",
      "author": "Uetz",
      "year": "2000",
      "journal-title": "Nature"
    },
    {
      "key": "key\n\t\t\t\t20170408045537_B47",
      "doi-asserted-by": "crossref",
      "first-page": "11121",
      "DOI": "10.1016/S0021-9258(19)78100-9",
      "article-title": "Recombinant replication protein A: expression, complex formation, and functional characterization",
      "volume": "269",
      "author": "Henricksen",
      "year": "1994",
      "journal-title": "J. Biol. Chem"
    }
  ],
  "container-title": "Nucleic Acids Research",
  "original-title": [],
  "language": "en",
  "link": [
    {
      "URL": "http://academic.oup.com/nar/article-pdf/34/19/5541/11216356/gkl685.pdf",
      "content-type": "unspecified",
      "content-version": "vor",
      "intended-application": "similarity-checking"
    }
  ],
  "deposited": {
    "date-parts": [
      [
        2021,
        8,
        1
      ]
    ],
    "date-time": "2021-08-01T20:49:14Z",
    "timestamp": 1627850954000
  },
  "score": 1,
  "resource": {
    "primary": {
      "URL": "https://academic.oup.com/nar/article-lookup/doi/10.1093/nar/gkl685"
    }
  },
  "subtitle": [],
  "short-title": [],
  "issued": {
    "date-parts": [
      [
        2006,
        10,
        4
      ]
    ]
  },
  "references-count": 47,
  "journal-issue": {
    "issue": "19",
    "published-online": {
      "date-parts": [
        [
          2006,
          10,
          4
        ]
      ]
    },
    "published-print": {
      "date-parts": [
        [
          2006,
          11
        ]
      ]
    }
  },
  "URL": "http://dx.doi.org/10.1093/nar/gkl685",
  "relation": {},
  "ISSN": [
    "0305-1048",
    "1362-4962"
  ],
  "subject": [],
  "published": {
    "date-parts": [
      [
        2006,
        10,
        4
      ]
    ]
  }
}