DOI: 10.1093/genetics/97.2.307. A SECOND INFORMATIONAL SUPPRESSOR, <i>SUP-7 X</i>, IN <i>CAENORHABDITIS ELEGANS</i>

A SECOND INFORMATIONAL SUPPRESSOR, SUP-7 X, IN CAENORHABDITIS ELEGANS

10.1093/genetics/97.2.307

Crossref journal-article

Oxford University Press (OUP)

Genetics (286)

Abstract

ABSTRACT More than 30 independent suppressor mutations have been obtained in the nematode C. elegans through reversion analysis of two unc-13 mutants. Many of the new isolates map to the region of the previously identified informational suppressor, sup-5 III (Waterston and Brenner 1978). Several of the other suppressor mutations map to the left half of the X-linkage group and define a second suppressor gene, sup-7 X. In tests against 40 mutations in six genes, the sup-7(st5) allele was found to suppress to a greater extent the same alleles acted on by sup-5(e1464). Like sup-5(e1464), sup-7(st5) acts on null alleles of the myosin heavy-chain gene unc-54 I (MacLeod et al. 1977; MacLeod, Waterston and Brenner 1977) and the putative paramyosin gene unc-15 I (Waterston et al. 1977). Chemical analysis of unc-15(e1214); sup-7(st5) animals show that paramyosin is restored to more than 30% of the wild-type level. —As was observed for sup-5(e1464), suppression by sup-7(st5) is dose dependent and is greater in animals grown at 15° than at 25°. However, associated with this increased suppression is a decreased viability of sup-7(st5) homozygotes. Reversion of the lethality has resulted in the isolation of deficiency mutations that complement st5 lethality, but lack suppressor function. These properties of sup-7(st5) suggest that it, like sup-5(e1464), is an informational suppressor of null alleles, and its reversion via deficiencies further narrows the possible explanations of its action.

Bibliography

Waterston, R. H. (1981). A SECOND INFORMATIONAL SUPPRESSOR, SUP-7 X, IN CAENORHABDITIS ELEGANS. Genetics, 97(2), 307â325.

Authors 1

Robert H Waterston (first)

References 0 Referenced 45

None

Dates

Type	When
Created	4 years, 4 months ago (April 22, 2021, 12:29 p.m.)
Deposited	4 years, 4 months ago (April 22, 2021, 12:29 p.m.)
Indexed	1 year, 7 months ago (Jan. 13, 2024, 4:19 a.m.)
Issued	44 years, 6 months ago (Feb. 1, 1981)
Published	44 years, 6 months ago (Feb. 1, 1981)
Published Online	44 years, 6 months ago (Feb. 1, 1981)
Published Print	44 years, 6 months ago (Feb. 1, 1981)

Funders 0

None

BibTeX

@article{Waterston_1981, title={A SECOND INFORMATIONAL SUPPRESSOR, SUP-7 X, IN CAENORHABDITIS ELEGANS}, volume={97}, ISSN={1943-2631}, url={http://dx.doi.org/10.1093/genetics/97.2.307}, DOI={10.1093/genetics/97.2.307}, number={2}, journal={Genetics}, publisher={Oxford University Press (OUP)}, author={Waterston, Robert H}, year={1981}, month=feb, pages={307–325} }

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  "abstract": "<jats:title>ABSTRACT</jats:title>\n               <jats:p>More than 30 independent suppressor mutations have been obtained in the nematode C. elegans through reversion analysis of two unc-13 mutants. Many of the new isolates map to the region of the previously identified informational suppressor, sup-5 III (Waterston and Brenner 1978). Several of the other suppressor mutations map to the left half of the X-linkage group and define a second suppressor gene, sup-7 X. In tests against 40 mutations in six genes, the sup-7(st5) allele was found to suppress to a greater extent the same alleles acted on by sup-5(e1464). Like sup-5(e1464), sup-7(st5) acts on null alleles of the myosin heavy-chain gene unc-54 I (MacLeod et al. 1977; MacLeod, Waterston and Brenner 1977) and the putative paramyosin gene unc-15 I (Waterston et al. 1977). Chemical analysis of unc-15(e1214); sup-7(st5) animals show that paramyosin is restored to more than 30% of the wild-type level. \u2014As was observed for sup-5(e1464), suppression by sup-7(st5) is dose dependent and is greater in animals grown at 15\u00b0 than at 25\u00b0. However, associated with this increased suppression is a decreased viability of sup-7(st5) homozygotes. Reversion of the lethality has resulted in the isolation of deficiency mutations that complement st5 lethality, but lack suppressor function. These properties of sup-7(st5) suggest that it, like sup-5(e1464), is an informational suppressor of null alleles, and its reversion via deficiencies further narrows the possible explanations of its action.</jats:p>",
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