DOI: 10.1093/genetics/91.2.245. EVIDENCE OF THE INSENSITIVITY OF THE α-<i>inc</i> ALLELE TO THE FUNCTION OF THE HOMOTHALLIC GENES IN SACCHAROMYCES YEASTS

EVIDENCE OF THE INSENSITIVITY OF THE α-inc ALLELE TO THE FUNCTION OF THE HOMOTHALLIC GENES IN SACCHAROMYCES YEASTS

10.1093/genetics/91.2.245

Crossref journal-article

Oxford University Press (OUP)

Genetics (286)

Abstract

ABSTRACT The possible function of the α-inc allele (an α mating-type allele that is insensitive to the function of the homothallic gene system) was investigated by means of protoplast fusion. The fusion of protoplasts prepared from haploid strains of α-inc HO HMα HMa and α ho hmα HMa gave rise mainly to nonmating clones (58 of 64 isolates) and a few clones (six of 64 isolates) showing a mating type. Thirty of the 56 nonmating clones showed the diploid cell size and 28 clones had a larger cell size. Tetrad analysis of the nonmating clones with diploid cell size indicated that they were a/α-inc diploid; the normal α allele in α/α-inc cells was preferentially switched to an a allele. This observation further indicated that the HO/ho HMα/hmα HMa/HMa genotype is effective for the conversion of the α to a and that the inconvertibility of the a-inc allele is due to the insensitivity of the mating-type allele to the functional combination of the homothallic genes. It was suspected that fusion products larger than diploid cells might have been caused by multiple fusion of protoplasts.

Bibliography

Takano, I., & Arima, K. (1979). EVIDENCE OF THE INSENSITIVITY OF THE Î±-inc ALLELE TO THE FUNCTION OF THE HOMOTHALLIC GENES IN SACCHAROMYCES YEASTS. Genetics, 91(2), 245â254.

Authors 2

Isamu Takano (first)
Kenji Arima (additional)

References 0 Referenced 8

None

Dates

Type	When
Created	4 years, 4 months ago (April 22, 2021, 6:22 a.m.)
Deposited	4 years, 4 months ago (April 22, 2021, 6:22 a.m.)
Indexed	2 years, 4 months ago (April 1, 2023, 2:28 a.m.)
Issued	46 years, 6 months ago (Feb. 1, 1979)
Published	46 years, 6 months ago (Feb. 1, 1979)
Published Online	46 years, 6 months ago (Feb. 1, 1979)
Published Print	46 years, 6 months ago (Feb. 1, 1979)

Funders 0

None

BibTeX

@article{Takano_1979, title={EVIDENCE OF THE INSENSITIVITY OF THE α-inc ALLELE TO THE FUNCTION OF THE HOMOTHALLIC GENES IN SACCHAROMYCES YEASTS}, volume={91}, ISSN={1943-2631}, url={http://dx.doi.org/10.1093/genetics/91.2.245}, DOI={10.1093/genetics/91.2.245}, number={2}, journal={Genetics}, publisher={Oxford University Press (OUP)}, author={Takano, Isamu and Arima, Kenji}, year={1979}, month=feb, pages={245–254} }

JSON

{
  "indexed": {
    "date-parts": [
      [
        2023,
        4,
        1
      ]
    ],
    "date-time": "2023-04-01T06:28:52Z",
    "timestamp": 1680330532552
  },
  "reference-count": 0,
  "publisher": "Oxford University Press (OUP)",
  "issue": "2",
  "license": [
    {
      "start": {
        "date-parts": [
          [
            1979,
            2,
            1
          ]
        ],
        "date-time": "1979-02-01T00:00:00Z",
        "timestamp": 286675200000
      },
      "content-version": "vor",
      "delay-in-days": 0,
      "URL": "https://academic.oup.com/journals/pages/open_access/funder_policies/chorus/standard_publication_model"
    }
  ],
  "content-domain": {
    "domain": [],
    "crossmark-restriction": false
  },
  "published-print": {
    "date-parts": [
      [
        1979,
        2,
        1
      ]
    ]
  },
  "abstract": "<jats:title>ABSTRACT</jats:title>\n               <jats:p>The possible function of the \u03b1-inc allele (an \u03b1 mating-type allele that is insensitive to the function of the homothallic gene system) was investigated by means of protoplast fusion. The fusion of protoplasts prepared from haploid strains of \u03b1-inc \u00a0HO HM\u03b1 HMa and \u03b1 ho hm\u03b1 HMa gave rise mainly to nonmating clones (58 of 64 isolates) and a few clones (six of 64 isolates) showing a mating type. Thirty of the 56 nonmating clones showed the diploid cell size and 28 clones had a larger cell size. Tetrad analysis of the nonmating clones with diploid cell size indicated that they were a/\u03b1-inc diploid; the normal \u03b1 allele in \u03b1/\u03b1-inc cells was preferentially switched to an a allele. This observation further indicated that the HO/ho HM\u03b1/hm\u03b1 HMa/HMa genotype is effective for the conversion of the \u03b1 to a and that the inconvertibility of the a-inc allele is due to the insensitivity of the mating-type allele to the functional combination of the homothallic genes. It was suspected that fusion products larger than diploid cells might have been caused by multiple fusion of protoplasts.</jats:p>",
  "DOI": "10.1093/genetics/91.2.245",
  "type": "journal-article",
  "created": {
    "date-parts": [
      [
        2021,
        4,
        22
      ]
    ],
    "date-time": "2021-04-22T10:22:21Z",
    "timestamp": 1619086941000
  },
  "page": "245-254",
  "source": "Crossref",
  "is-referenced-by-count": 8,
  "title": "EVIDENCE OF THE INSENSITIVITY OF THE \u03b1-<i>inc</i> ALLELE TO THE FUNCTION OF THE HOMOTHALLIC GENES IN SACCHAROMYCES YEASTS",
  "prefix": "10.1093",
  "volume": "91",
  "author": [
    {
      "given": "Isamu",
      "family": "Takano",
      "sequence": "first",
      "affiliation": [
        {
          "name": "The Central Research Institute, Suntory Ltd., Wakayamadai, Shimarmoto-cho, Mishima-gun, Osaka 618, Japan"
        }
      ]
    },
    {
      "given": "Kenji",
      "family": "Arima",
      "sequence": "additional",
      "affiliation": [
        {
          "name": "The Central Research Institute, Suntory Ltd., Wakayamadai, Shimarmoto-cho, Mishima-gun, Osaka 618, Japan"
        }
      ]
    }
  ],
  "member": "286",
  "published-online": {
    "date-parts": [
      [
        1979,
        2,
        1
      ]
    ]
  },
  "container-title": "Genetics",
  "original-title": [],
  "language": "en",
  "link": [
    {
      "URL": "http://academic.oup.com/genetics/article-pdf/91/2/245/34424455/genetics0245.pdf",
      "content-type": "application/pdf",
      "content-version": "vor",
      "intended-application": "syndication"
    },
    {
      "URL": "http://academic.oup.com/genetics/article-pdf/91/2/245/34424455/genetics0245.pdf",
      "content-type": "unspecified",
      "content-version": "vor",
      "intended-application": "similarity-checking"
    }
  ],
  "deposited": {
    "date-parts": [
      [
        2021,
        4,
        22
      ]
    ],
    "date-time": "2021-04-22T10:22:22Z",
    "timestamp": 1619086942000
  },
  "score": 1,
  "resource": {
    "primary": {
      "URL": "https://academic.oup.com/genetics/article/91/2/245/5993344"
    }
  },
  "subtitle": [],
  "short-title": [],
  "issued": {
    "date-parts": [
      [
        1979,
        2,
        1
      ]
    ]
  },
  "references-count": 0,
  "journal-issue": {
    "issue": "2",
    "published-print": {
      "date-parts": [
        [
          1979,
          2,
          1
        ]
      ]
    }
  },
  "URL": "http://dx.doi.org/10.1093/genetics/91.2.245",
  "relation": {},
  "ISSN": [
    "1943-2631"
  ],
  "subject": [],
  "published-other": {
    "date-parts": [
      [
        1979,
        2,
        1
      ]
    ]
  },
  "published": {
    "date-parts": [
      [
        1979,
        2,
        1
      ]
    ]
  }
}