DOI: 10.1093/genetics/86.4.745. FRAMESHIFTS AND FRAMESHIFT SUPPRESSORS IN <i>SACCHAROMYCES CEREVISIAE</i>

FRAMESHIFTS AND FRAMESHIFT SUPPRESSORS IN SACCHAROMYCES CEREVISIAE

10.1093/genetics/86.4.745

Crossref journal-article

Oxford University Press (OUP)

Genetics (286)

Abstract

ABSTRACT Using ICR-170 as a mutagen, we have induced a set of mutations in yeast which exhibit behavior similar to that shown for bacterial frameshift mutations. Our genetic study shows that these mutations are polar; the polarity can be relieved by internal suppressors; they revert with acridine half-mustards and are not suppressed by known nonsense suppressors. However, they are suppressed by other dominant external suppressors, which fall into two mutually exclusive groups. Five genetically distinct suppressors were obtained for one of these groups, using co-reversion of two frameshift markers. Three of these are lethal in combination with each other and show a reduction in the GLY3 tRNA peak on a Sepharose 4B column. A fourth suppressor shows an altered chromatographic profile for GLY1 tRNA. We suggest that this group of suppressors represent mutations in the structural genes for the isoaccepting glycyl-tRNA's. Two other suppressors (one linked to the centromere of chromosome III) were found to suppress a second group of frameshifts. Genetic and biochemical studies show that the nonMendelian factor [PSI+] increases the efficiency of some frameshift suppressors.

Bibliography

Culbertson, M. R., Charnas, L., Johnson, M. T., & Fink, G. R. (1977). FRAMESHIFTS AND FRAMESHIFT SUPPRESSORS IN SACCHAROMYCES CEREVISIAE. Genetics, 86(4), 745â764.

Authors 4

Michael R Culbertson (first)
Lawrence Charnas (additional)
M Tina Johnson (additional)
Gerald R Fink (additional)

References 0 Referenced 76

None

Dates

Type	When
Created	4 years, 3 months ago (April 21, 2021, 8:48 p.m.)
Deposited	4 years, 3 months ago (April 21, 2021, 8:48 p.m.)
Indexed	2 weeks ago (Aug. 7, 2025, 4:57 p.m.)
Issued	48 years ago (Aug. 1, 1977)
Published	48 years ago (Aug. 1, 1977)
Published Online	48 years ago (Aug. 1, 1977)
Published Print	48 years ago (Aug. 1, 1977)

Funders 0

None

BibTeX

@article{Culbertson_1977, title={FRAMESHIFTS AND FRAMESHIFT SUPPRESSORS IN SACCHAROMYCES CEREVISIAE}, volume={86}, ISSN={1943-2631}, url={http://dx.doi.org/10.1093/genetics/86.4.745}, DOI={10.1093/genetics/86.4.745}, number={4}, journal={Genetics}, publisher={Oxford University Press (OUP)}, author={Culbertson, Michael R and Charnas, Lawrence and Johnson, M Tina and Fink, Gerald R}, year={1977}, month=aug, pages={745–764} }

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