DOI: 10.1093/genetics/163.3.931. The <i>Drosophila melanogaster sir2</i>+ Gene Is Nonessential and Has Only Minor Effects on Position-Effect Variegation

The Drosophila melanogaster sir2+ Gene Is Nonessential and Has Only Minor Effects on Position-Effect Variegation

10.1093/genetics/163.3.931

Crossref journal-article

Oxford University Press (OUP)

Genetics (286)

Abstract

Abstract Five Drosophila melanogaster genes belong to the highly conserved sir2 family, which encodes NAD+-dependent protein deacetylases. Of these five, dsir2+ (CG5216) is most similar to the Saccharomyces cerevisiae SIR2 gene, which has profound effects on chromatin structure and life span. Four independent Drosophila strains were found with P-element insertions near the dsir2 transcriptional start site as well as extraneous linked recessive lethal mutations. Imprecise excision of one of these P elements (PlacW 07223) from a chromosome freed of extraneous lethal mutations produced dsir217, a null intragenic deletion allele that generates no DSIR2 protein. Contrary to expectations from the report by Rosenberg and Parkhurst on their P-mobilization allele dSir2ex10, homozygosity for dsir217 had no apparent deleterious effects on viability, developmental rate, or sex ratio, and it fully complemented sir2ex10. Moreover, through a genetic test, we ruled out the reported effect of dSir2ex10 on Sex-lethal expression. We did observe a modest, strictly recessive suppression of whitem4 position-effect variegation and a shortening of life span in dsir2 homozygous mutants, suggesting that dsir2 has some functions in common with yeast SIR2.

Bibliography

ÃstrÃ¶m, S. U., Cline, T. W., & Rine, J. (2003). The Drosophila melanogaster sir2+ Gene Is Nonessential and Has Only Minor Effects on Position-Effect Variegation. Genetics, 163(3), 931â937.

Authors 3

Stefan U Åström (first)
Thomas W Cline (additional)
Jasper Rine (additional)

References 34 Referenced 68

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10.1006/bbrc.1999.0897 / Biochem. Biophys. Res. Commun. / Characterization of five human cDNAs with homology to the yeast SIR2 gene: Sir2-like proteins (sirtuins) metabolize NAD and may have protein ADP-ribosyltransferase activity by Frye (1999)
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Dates

Type	When
Created	4 years, 4 months ago (April 23, 2021, 1:36 a.m.)
Deposited	3 years, 7 months ago (Jan. 5, 2022, 2:20 p.m.)
Indexed	3 weeks ago (Aug. 7, 2025, 4:51 p.m.)
Issued	22 years, 5 months ago (March 1, 2003)
Published	22 years, 5 months ago (March 1, 2003)
Published Online	22 years, 5 months ago (March 1, 2003)
Published Print	22 years, 5 months ago (March 1, 2003)

Funders 0

None

BibTeX

@article{_str_m_2003, title={The Drosophila melanogaster sir2+ Gene Is Nonessential and Has Only Minor Effects on Position-Effect Variegation}, volume={163}, ISSN={1943-2631}, url={http://dx.doi.org/10.1093/genetics/163.3.931}, DOI={10.1093/genetics/163.3.931}, number={3}, journal={Genetics}, publisher={Oxford University Press (OUP)}, author={Åström, Stefan U and Cline, Thomas W and Rine, Jasper}, year={2003}, month=mar, pages={931–937} }

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