DOI: 10.1093/genetics/162.1.113. A Targeted RNAi Screen for Genes Involved in Chromosome Morphogenesis and Nuclear Organization in the <i>Caenorhabditis elegans</i> Germline

A Targeted RNAi Screen for Genes Involved in Chromosome Morphogenesis and Nuclear Organization in the Caenorhabditis elegans Germline

10.1093/genetics/162.1.113

Crossref journal-article

Oxford University Press (OUP)

Genetics (286)

Abstract

Abstract We have implemented a functional genomics strategy to identify genes involved in chromosome morphogenesis and nuclear organization during meiotic prophase in the Caenorhabditis elegans germline. This approach took advantage of a gene-expression survey that used DNA microarray technology to identify genes preferentially expressed in the germline. We defined a subset of 192 germline-enriched genes whose expression profiles were similar to those of previously identified meiosis genes and designed a screen to identify genes for which inhibition by RNA interference (RNAi) elicited defects in function or development of the germline. We obtained strong germline phenotypes for 27% of the genes tested, indicating that this targeted approach greatly enriched for genes that function in the germline. In addition to genes involved in key meiotic prophase events, we identified genes involved in meiotic progression, germline proliferation, and chromosome organization and/or segregation during mitotic growth.

Bibliography

ColaiÃ¡covo, M. P., Stanfield, G. M., Reddy, K. C., Reinke, V., Kim, S. K., & Villeneuve, A. M. (2002). A Targeted RNAi Screen for Genes Involved in Chromosome Morphogenesis and Nuclear Organization in the Caenorhabditis elegans Germline. Genetics, 162(1), 113â128.

Authors 6

M P Colaiácovo (first)
G M Stanfield (additional)
K C Reddy (additional)
V Reinke (additional)
S K Kim (additional)
A M Villeneuve (additional)

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Dates

Type	When
Created	4 years, 4 months ago (April 23, 2021, 1:01 a.m.)
Deposited	3 years, 7 months ago (Jan. 5, 2022, 12:47 p.m.)
Indexed	1 year, 3 months ago (May 22, 2024, 9:54 a.m.)
Issued	23 years ago (Sept. 1, 2002)
Published	23 years ago (Sept. 1, 2002)
Published Online	23 years ago (Sept. 1, 2002)
Published Print	23 years ago (Sept. 1, 2002)

Funders 0

None

BibTeX

@article{Colai_covo_2002, title={A Targeted RNAi Screen for Genes Involved in Chromosome Morphogenesis and Nuclear Organization in the Caenorhabditis elegans Germline}, volume={162}, ISSN={1943-2631}, url={http://dx.doi.org/10.1093/genetics/162.1.113}, DOI={10.1093/genetics/162.1.113}, number={1}, journal={Genetics}, publisher={Oxford University Press (OUP)}, author={Colaiácovo, M P and Stanfield, G M and Reddy, K C and Reinke, V and Kim, S K and Villeneuve, A M}, year={2002}, month=sep, pages={113–128} }

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