DOI: 10.1093/genetics/161.3.1015. Enhancement of <i>Saccharomyces cerevisiae</i> End-Joining Efficiency by Cell Growth Stage but Not by Impairment of Recombination

Enhancement of Saccharomyces cerevisiae End-Joining Efficiency by Cell Growth Stage but Not by Impairment of Recombination

10.1093/genetics/161.3.1015

Crossref journal-article

Oxford University Press (OUP)

Genetics (286)

Abstract

Abstract Cells can repair DNA double-strand breaks by both homologous and nonhomologous mechanisms. To explore the basis of pathway utilization, we developed both plasmid and chromosomal yeast repair assays in which breaks are created with restriction endonucleases so that nonhomologous end-joining (NHEJ) competes with the single-strand annealing (SSA) recombination pathway, which we show acts with high efficiency via terminal direct repeats of only 28 bp and with reduced but measurable efficiency at 10 bp. The chromosomal assay utilizes a novel approach termed suicide deletion in which the endonuclease cleaves its own gene from the chromosome, thereby ending the futile cleavage cycle that otherwise prevents detection of simple-religation events. Eliminating SSA as a possibility in either assay, either by removal of the direct repeat or by mutation of RAD52, increased the relative but not the absolute efficiency of NHEJ. In contrast, the apparent efficiency of NHEJ was specifically increased in the G1 stage of the haploid cell cycle, as well as by the glucose depletion-signaled transition to stationary phase. The combined results argue against a model in which pathway utilization is determined by a passive competition. Instead, they demonstrate an active regulation designed to optimize the likelihood of genome restoration based on cell state.

Bibliography

Karathanasis, E., & Wilson, T. E. (2002). Enhancement of Saccharomyces cerevisiae End-Joining Efficiency by Cell Growth Stage but Not by Impairment of Recombination. Genetics, 161(3), 1015â1027.

Authors 2

Elissa Karathanasis (first)
Thomas E Wilson (additional)

References 40 Referenced 89

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Dates

Type	When
Created	4 years, 4 months ago (April 23, 2021, 12:57 a.m.)
Deposited	3 years, 7 months ago (Jan. 5, 2022, 9:09 a.m.)
Indexed	3 months ago (May 27, 2025, 2:47 a.m.)
Issued	23 years, 1 month ago (July 1, 2002)
Published	23 years, 1 month ago (July 1, 2002)
Published Online	23 years, 1 month ago (July 1, 2002)
Published Print	23 years, 1 month ago (July 1, 2002)

Funders 0

None

BibTeX

@article{Karathanasis_2002, title={Enhancement of Saccharomyces cerevisiae End-Joining Efficiency by Cell Growth Stage but Not by Impairment of Recombination}, volume={161}, ISSN={1943-2631}, url={http://dx.doi.org/10.1093/genetics/161.3.1015}, DOI={10.1093/genetics/161.3.1015}, number={3}, journal={Genetics}, publisher={Oxford University Press (OUP)}, author={Karathanasis, Elissa and Wilson, Thomas E}, year={2002}, month=jul, pages={1015–1027} }

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