DOI: 10.1093/genetics/159.3.919. A Filamentous Growth Response Mediated by the Yeast Mating Pathway

A Filamentous Growth Response Mediated by the Yeast Mating Pathway

10.1093/genetics/159.3.919

Crossref journal-article

Oxford University Press (OUP)

Genetics (286)

Abstract

Abstract Haploid cells of the budding yeast Saccharomyces cerevisiae respond to mating pheromones by arresting their cell-division cycle in G1 and differentiating into a cell type capable of locating and fusing with mating partners. Yeast cells undergo chemotactic cell surface growth when pheromones are present above a threshold level for morphogenesis; however, the morphogenetic responses of cells to levels of pheromone below this threshold have not been systematically explored. Here we show that MATa haploid cells exposed to low levels of the α-factor mating pheromone undergo a novel cellular response: cells modulate their division patterns and cell shape, forming colonies composed of filamentous chains of cells. Time-lapse analysis of filament formation shows that its dynamics are distinct from that of pseudohyphal growth; during pheromone-induced filament formation, daughter cells are delayed relative to mother cells with respect to the timing of bud emergence. Filament formation requires the RSR1(BUD1), BUD8, SLK1/BCK1, and SPA2 genes and many elements of the STE11/STE7 MAP kinase pathway; this response is also independent of FAR1, a gene involved in orienting cell polarization during the mating response. We suggest that mating yeast cells undergo a complex response to low levels of pheromone that may enhance the ability of cells to search for mating partners through the modification of cell shape and alteration of cell-division patterns.

Bibliography

Erdman, S., & Snyder, M. (2001). A Filamentous Growth Response Mediated by the Yeast Mating Pathway. Genetics, 159(3), 919â928.

Authors 2

Scott Erdman (first)
Michael Snyder (additional)

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Dates

Type	When
Created	4 years, 4 months ago (April 28, 2021, 12:06 a.m.)
Deposited	3 years, 7 months ago (Jan. 5, 2022, 2:16 a.m.)
Indexed	4 months, 2 weeks ago (April 11, 2025, 3:24 p.m.)
Issued	23 years, 9 months ago (Nov. 1, 2001)
Published	23 years, 9 months ago (Nov. 1, 2001)
Published Online	23 years, 9 months ago (Nov. 1, 2001)
Published Print	23 years, 9 months ago (Nov. 1, 2001)

Funders 0

None

BibTeX

@article{Erdman_2001, title={A Filamentous Growth Response Mediated by the Yeast Mating Pathway}, volume={159}, ISSN={1943-2631}, url={http://dx.doi.org/10.1093/genetics/159.3.919}, DOI={10.1093/genetics/159.3.919}, number={3}, journal={Genetics}, publisher={Oxford University Press (OUP)}, author={Erdman, Scott and Snyder, Michael}, year={2001}, month=nov, pages={919–928} }

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