DOI: 10.1093/genetics/159.2.453. Genes Involved in Sister Chromatid Separation and Segregation in the Budding Yeast <i>Saccharomyces cerevisiae</i>

Genes Involved in Sister Chromatid Separation and Segregation in the Budding Yeast Saccharomyces cerevisiae

10.1093/genetics/159.2.453

Crossref journal-article

Oxford University Press (OUP)

Genetics (286)

Abstract

Abstract Accurate chromosome segregation requires the precise coordination of events during the cell cycle. Replicated sister chromatids are held together while they are properly attached to and aligned by the mitotic spindle at metaphase. At anaphase, the links between sisters must be promptly dissolved to allow the mitotic spindle to rapidly separate them to opposite poles. To isolate genes involved in chromosome behavior during mitosis, we microscopically screened a temperature-sensitive collection of budding yeast mutants that contain a GFP-marked chromosome. Nine LOC (loss of cohesion) complementation groups that do not segregate sister chromatids at anaphase were identified. We cloned the corresponding genes and performed secondary tests to determine their function in chromosome behavior. We determined that three LOC genes, PDS1, ESP1, and YCS4, are required for sister chromatid separation and three other LOC genes, CSE4, IPL1, and SMT3, are required for chromosome segregation. We isolated alleles of two genes involved in splicing, PRP16 and PRP19, which impair α-tubulin synthesis thus preventing spindle assembly, as well as an allele of CDC7 that is defective in DNA replication. We also report an initial characterization of phenotypes associated with the SMT3/SUMO gene and the isolation of WSS1, a high-copy smt3 suppressor.

Bibliography

Biggins, S., Bhalla, N., Chang, A., Smith, D. L., & Murray, A. W. (2001). Genes Involved in Sister Chromatid Separation and Segregation in the Budding Yeast Saccharomyces cerevisiae. Genetics, 159(2), 453â470.

Authors 5

Sue Biggins (first)
Needhi Bhalla (additional)
Amy Chang (additional)
Dana L Smith (additional)
Andrew W Murray (additional)

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Dates

Type	When
Created	4 years, 4 months ago (April 28, 2021, 1:45 a.m.)
Deposited	3 years, 8 months ago (Jan. 5, 2022, 2:27 a.m.)
Indexed	1 month, 1 week ago (July 24, 2025, 7:02 a.m.)
Issued	23 years, 11 months ago (Oct. 1, 2001)
Published	23 years, 11 months ago (Oct. 1, 2001)
Published Online	23 years, 11 months ago (Oct. 1, 2001)
Published Print	23 years, 11 months ago (Oct. 1, 2001)

Funders 0

None

BibTeX

@article{Biggins_2001, title={Genes Involved in Sister Chromatid Separation and Segregation in the Budding Yeast Saccharomyces cerevisiae}, volume={159}, ISSN={1943-2631}, url={http://dx.doi.org/10.1093/genetics/159.2.453}, DOI={10.1093/genetics/159.2.453}, number={2}, journal={Genetics}, publisher={Oxford University Press (OUP)}, author={Biggins, Sue and Bhalla, Needhi and Chang, Amy and Smith, Dana L and Murray, Andrew W}, year={2001}, month=oct, pages={453–470} }

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