DOI: 10.1093/genetics/158.1.87. Multiple Functions of the Nonconserved N-Terminal Domain of Yeast TATA-Binding Protein

Multiple Functions of the Nonconserved N-Terminal Domain of Yeast TATA-Binding Protein

10.1093/genetics/158.1.87

Crossref journal-article

Oxford University Press (OUP)

Genetics (286)

Abstract

Abstract The TATA-binding protein (TBP) is composed of a highly conserved core domain sufficient for TATA-element binding and preinitiation complex formation as well as a highly divergent N-terminal region that is dispensable for yeast cell viability. In vitro, removal of the N-terminal region domain enhances TBP-TATA association and TBP dimerization. Here, we examine the effects of truncation of the N-terminal region in the context of yeast TBP mutants with specific defects in DNA binding and in interactions with various proteins. For a subset of mutations that disrupt DNA binding and the response to transcriptional activators, removal of the N-terminal domain rescues their transcriptional defects. By contrast, deletion of the N-terminal region is lethal in combination with mutations on a limited surface of TBP. Although this surface is important for interactions with TFIIA and Brf1, TBP interactions with these two factors do not appear to be responsible for this dependence on the N-terminal region. Our results suggest that the N-terminal region of TBP has at least two distinct functions in vivo. It inhibits the interaction of TBP with TATA elements, and it acts positively in combination with a specific region of the TBP core domain that presumably interacts with another protein(s).

Bibliography

Lee, M., & Struhl, K. (2001). Multiple Functions of the Nonconserved N-Terminal Domain of Yeast TATA-Binding Protein. Genetics, 158(1), 87â93.

Authors 2

Mark Lee (first)
Kevin Struhl (additional)

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Dates

Type	When
Created	4 years, 4 months ago (April 28, 2021, 1:40 a.m.)
Deposited	3 years, 7 months ago (Jan. 5, 2022, 12:38 a.m.)
Indexed	1 year, 6 months ago (Feb. 12, 2024, 8:43 p.m.)
Issued	24 years, 3 months ago (May 1, 2001)
Published	24 years, 3 months ago (May 1, 2001)
Published Online	24 years, 3 months ago (May 1, 2001)
Published Print	24 years, 3 months ago (May 1, 2001)

Funders 0

None

BibTeX

@article{Lee_2001, title={Multiple Functions of the Nonconserved N-Terminal Domain of Yeast TATA-Binding Protein}, volume={158}, ISSN={1943-2631}, url={http://dx.doi.org/10.1093/genetics/158.1.87}, DOI={10.1093/genetics/158.1.87}, number={1}, journal={Genetics}, publisher={Oxford University Press (OUP)}, author={Lee, Mark and Struhl, Kevin}, year={2001}, month=may, pages={87–93} }

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