DOI: 10.1093/genetics/154.3.1039. Subtelomeric Repeat Amplification Is Associated With Growth at Elevated Temperature in yku70 Mutants of Saccharomyces cerevisiae

Subtelomeric Repeat Amplification Is Associated With Growth at Elevated Temperature in yku70 Mutants of Saccharomyces cerevisiae

10.1093/genetics/154.3.1039

Crossref journal-article

Oxford University Press (OUP)

Genetics (286)

Abstract

Abstract Inactivation of the Saccharomyces cerevisiae gene YKU70 (HDF1), which encodes one subunit of the Ku heterodimer, confers a DNA double-strand break repair defect, shortening of and structural alterations in the telomeres, and a severe growth defect at 37°. To elucidate the basis of the temperature sensitivity, we analyzed subclones derived from rare yku70 mutant cells that formed a colony when plated at elevated temperature. In all these temperature-resistant subclones, but not in cell populations shifted to 37°, we observed substantial amplification and redistribution of subtelomeric Y′ element DNA. Amplification of Y′ elements and adjacent telomeric sequences has been described as an alternative pathway for chromosome end stabilization that is used by postsenescence survivors of mutants deficient for the telomerase pathway. Our data suggest that the combination of Ku deficiency and elevated temperature induces a potentially lethal alteration of telomere structure or function. Both in yku70 mutants and in wild type, incubation at 37° results in a slight reduction of the mean length of terminal restriction fragments, but not in a significant loss of telomeric (C1-3A/TG1-3)n sequences. We propose that the absence of Ku, which is known to bind to telomeres, affects the telomeric chromatin so that its chromosome end-defining function is lost at 37°.

Bibliography

Fellerhoff, B., Eckardt-Schupp, F., & Friedl, A. A. (2000). Subtelomeric Repeat Amplification Is Associated With Growth at Elevated Temperature in yku70 Mutants of Saccharomyces cerevisiae. Genetics, 154(3), 1039â1051.

Authors 3

Barbara Fellerhoff (first)
Friederike Eckardt-Schupp (additional)
Anna A Friedl (additional)

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Dates

Type	When
Created	4 years, 4 months ago (April 27, 2021, 11:21 p.m.)
Deposited	3 years, 8 months ago (Jan. 3, 2022, 11:34 p.m.)
Indexed	1 month, 1 week ago (July 24, 2025, 7:43 a.m.)
Issued	25 years, 6 months ago (March 1, 2000)
Published	25 years, 6 months ago (March 1, 2000)
Published Online	25 years, 6 months ago (March 1, 2000)
Published Print	25 years, 6 months ago (March 1, 2000)

Funders 0

None

BibTeX

@article{Fellerhoff_2000, title={Subtelomeric Repeat Amplification Is Associated With Growth at Elevated Temperature in yku70 Mutants of Saccharomyces cerevisiae}, volume={154}, ISSN={1943-2631}, url={http://dx.doi.org/10.1093/genetics/154.3.1039}, DOI={10.1093/genetics/154.3.1039}, number={3}, journal={Genetics}, publisher={Oxford University Press (OUP)}, author={Fellerhoff, Barbara and Eckardt-Schupp, Friederike and Friedl, Anna A}, year={2000}, month=mar, pages={1039–1051} }

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