DOI: 10.1093/genetics/153.3.1245. RNA Interference Can Target Pre-mRNA: Consequences for Gene Expression in a Caenorhabditis elegans Operon

RNA Interference Can Target Pre-mRNA: Consequences for Gene Expression in a Caenorhabditis elegans Operon

10.1093/genetics/153.3.1245

Crossref journal-article

Oxford University Press (OUP)

Genetics (286)

Abstract

Abstract In nematodes, flies, trypanosomes, and planarians, introduction of double-stranded RNA results in sequence-specific inactivation of gene function, a process termed RNA interference (RNAi). We demonstrate that RNAi against the Caenorhabditis elegans gene lir-1, which is part of the lir-1/lin-26 operon, induced phenotypes very different from a newly isolated lir-1 null mutation. Specifically, lir-1(RNAi) induced embryonic lethality reminiscent of moderately strong lin-26 alleles, whereas the lir-1 null mutant was viable. We show that the lir-1(RNAi) phenotypes resulted from a severe loss of lin-26 gene expression. In addition, we found that RNAi directed against lir-1 or lin-26 introns induced similar phenotypes, so we conclude that lir-1(RNAi) targets the lir-1/lin-26 pre-mRNA. This provides direct evidence that RNA interference can prevent gene expression by targeting nuclear transcripts. Our results highlight that caution may be necessary when interpreting RNA interference without the benefit of mutant alleles.

Bibliography

Bosher, J. M., Dufourcq, P., Sookhareea, S., & Labouesse, M. (1999). RNA Interference Can Target Pre-mRNA: Consequences for Gene Expression in a Caenorhabditis elegans Operon. Genetics, 153(3), 1245â1256.

Authors 4

Julia M Bosher (first)
Pascale Dufourcq (additional)
Satis Sookhareea (additional)
Michel Labouesse (additional)

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Dates

Type	When
Created	4 years, 4 months ago (April 28, 2021, 1:10 a.m.)
Deposited	3 years, 8 months ago (Jan. 3, 2022, 10:54 p.m.)
Indexed	4 months, 4 weeks ago (April 5, 2025, 5:44 p.m.)
Issued	25 years, 10 months ago (Nov. 1, 1999)
Published	25 years, 10 months ago (Nov. 1, 1999)
Published Online	25 years, 10 months ago (Nov. 1, 1999)
Published Print	25 years, 10 months ago (Nov. 1, 1999)

Funders 0

None

BibTeX

@article{Bosher_1999, title={RNA Interference Can Target Pre-mRNA: Consequences for Gene Expression in a Caenorhabditis elegans Operon}, volume={153}, ISSN={1943-2631}, url={http://dx.doi.org/10.1093/genetics/153.3.1245}, DOI={10.1093/genetics/153.3.1245}, number={3}, journal={Genetics}, publisher={Oxford University Press (OUP)}, author={Bosher, Julia M and Dufourcq, Pascale and Sookhareea, Satis and Labouesse, Michel}, year={1999}, month=nov, pages={1245–1256} }

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