Temperature-Sensitive Mutations in the Saccharomyces cerevisiae MRT4, GRC5, SLA2 and THS1 Genes Result in Defects in mRNA Turnover
10.1093/genetics/153.1.35
Crossref journal-article
Oxford University Press (OUP)
Genetics (286)
Abstract

Abstract In a screen for factors involved in mRNA turnover, four temperature-sensitive yeast strains (ts1189, ts942, ts817, and ts1100) exhibited defects in the decay of several mRNAs. Complementation of the growth and mRNA decay defects, and genetic experiments, revealed that ts1189 is mutated in the previously unknown MRT4 gene, ts942 is mutated in GRC5 (encoding the L9 ribosomal protein), ts817 contains a mutation in SLA2 (encoding a membrane protein), and ts1100 contains a mutation in THS1 (encoding the threonyl-tRNA synthetase). Three of the four mutants (mrt4, grc5, and sla2) were not defective in protein synthesis, suggesting that these strains contain mutations in factors that may play a specific role in mRNA decay. The mRNA stabilization observed in the ths1 strain, however, could be due to the significant drop in translation observed in this mutant at 37°. While the three interesting mutants appear to encode novel mRNA decay factors, at least one could be linked to a previously characterized mRNA decay pathway. The growth and mRNA decay defects of ts942 (grc5) cells were suppressed by overexpression of the NMD3 gene, encoding a protein shown to participate in a two-hybrid interaction with the nonsense-mediated decay protein Upf1p.

Bibliography

Zuk, D., Belk, J. P., & Jacobson, A. (1999). Temperature-Sensitive Mutations in the Saccharomyces cerevisiae MRT4, GRC5, SLA2 and THS1 Genes Result in Defects in mRNA Turnover. Genetics, 153(1), 35–47.

Authors 3
  1. Dorit Zuk (first)
  2. Jonathan P Belk (additional)
  3. Allan Jacobson (additional)
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Dates
Type When
Created 4 years, 3 months ago (April 27, 2021, 11:10 p.m.)
Deposited 3 years, 7 months ago (Jan. 3, 2022, 10:23 p.m.)
Indexed 3 weeks, 1 day ago (July 30, 2025, 8:42 p.m.)
Issued 25 years, 11 months ago (Sept. 1, 1999)
Published 25 years, 11 months ago (Sept. 1, 1999)
Published Online 25 years, 11 months ago (Sept. 1, 1999)
Published Print 25 years, 11 months ago (Sept. 1, 1999)
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@article{Zuk_1999, title={Temperature-Sensitive Mutations in the Saccharomyces cerevisiae MRT4, GRC5, SLA2 and THS1 Genes Result in Defects in mRNA Turnover}, volume={153}, ISSN={1943-2631}, url={http://dx.doi.org/10.1093/genetics/153.1.35}, DOI={10.1093/genetics/153.1.35}, number={1}, journal={Genetics}, publisher={Oxford University Press (OUP)}, author={Zuk, Dorit and Belk, Jonathan P and Jacobson, Allan}, year={1999}, month=sep, pages={35–47} }