DOI: 10.1093/genetics/152.1.221. lir-2, lir-1 and lin-26 Encode a New Class of Zinc-Finger Proteins and Are Organized in Two Overlapping Operons Both in Caenorhabditis elegans and in Caenorhabditis briggsae

lir-2, lir-1 and lin-26 Encode a New Class of Zinc-Finger Proteins and Are Organized in Two Overlapping Operons Both in Caenorhabditis elegans and in Caenorhabditis briggsae

10.1093/genetics/152.1.221

Crossref journal-article

Oxford University Press (OUP)

Genetics (286)

Abstract

Abstract lin-26, which encodes a unique Zn-finger protein, is required for differentiation of nonneuronal ectodermal cells in Caenorhabditis elegans. Here, we show that the two genes located immediately upstream of lin-26 encode LIN-26-like Zn-finger proteins; hence their names are lir-1 and lir-2 (lin-26 related). lir-2, lir-1, and lin-26 generate several isoforms by alternative splicing and/or trans-splicing at different positions. On the basis of their trans-splicing pattern, their intergenic distances, and their expression, we suggest that lir-2, lir-1, and lin-26 form two overlapping transcriptional operons. The first operon, which is expressed in virtually all cells, includes lir-2 and long lir-1 isoforms. The second operon, which is expressed in the nonneuronal ectoderm, includes short lir-1 isoforms, starting at exon 2 and lin-26. This unusual genomic organization has been conserved in C. briggsae, as shown by cloning the C. briggsae lir-2, lir-1, and lin-26 homologs. Particularly striking is the sequence conservation throughout the first lir-1 intron, which is very long in both species. Structural conservation is functionally meaningful as C. briggsae lin-26 is also expressed in the nonneuronal ectoderm and can complement a C. elegans lin-26 null mutation.

Bibliography

Dufourcq, P., Chanal, P., Vicaire, S., Camut, E., Quintin, S., Boer, B. G. W. den, Bosher, J. M., & Labouesse, M. (1999). lir-2, lir-1 and lin-26 Encode a New Class of Zinc-Finger Proteins and Are Organized in Two Overlapping Operons Both in Caenorhabditis elegans and in Caenorhabditis briggsae. Genetics, 152(1), 221â235.

Authors 8

Pascale Dufourcq (first)
Philippe Chanal (additional)
Serge Vicaire (additional)
Elise Camut (additional)
Sophie Quintin (additional)
Bart G W den Boer (additional)
Julia M Bosher (additional)
Michel Labouesse (additional)

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Dates

Type	When
Created	4 years, 4 months ago (April 27, 2021, 10:48 p.m.)
Deposited	3 years, 8 months ago (Jan. 3, 2022, 12:04 p.m.)
Indexed	2 months, 2 weeks ago (June 23, 2025, 9:09 a.m.)
Issued	26 years, 4 months ago (May 1, 1999)
Published	26 years, 4 months ago (May 1, 1999)
Published Online	26 years, 4 months ago (May 1, 1999)
Published Print	26 years, 4 months ago (May 1, 1999)

Funders 0

None

BibTeX

@article{Dufourcq_1999, title={lir-2, lir-1 and lin-26 Encode a New Class of Zinc-Finger Proteins and Are Organized in Two Overlapping Operons Both in Caenorhabditis elegans and in Caenorhabditis briggsae}, volume={152}, ISSN={1943-2631}, url={http://dx.doi.org/10.1093/genetics/152.1.221}, DOI={10.1093/genetics/152.1.221}, number={1}, journal={Genetics}, publisher={Oxford University Press (OUP)}, author={Dufourcq, Pascale and Chanal, Philippe and Vicaire, Serge and Camut, Elise and Quintin, Sophie and Boer, Bart G W den and Bosher, Julia M and Labouesse, Michel}, year={1999}, month=may, pages={221–235} }

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  "abstract": "<jats:title>Abstract</jats:title>\n               <jats:p>lin-26, which encodes a unique Zn-finger protein, is required for differentiation of nonneuronal ectodermal cells in Caenorhabditis elegans. Here, we show that the two genes located immediately upstream of lin-26 encode LIN-26-like Zn-finger proteins; hence their names are lir-1 and lir-2 (lin-26 related). lir-2, lir-1, and lin-26 generate several isoforms by alternative splicing and/or trans-splicing at different positions. On the basis of their trans-splicing pattern, their intergenic distances, and their expression, we suggest that lir-2, lir-1, and lin-26 form two overlapping transcriptional operons. The first operon, which is expressed in virtually all cells, includes lir-2 and long lir-1 isoforms. The second operon, which is expressed in the nonneuronal ectoderm, includes short lir-1 isoforms, starting at exon 2 and lin-26. This unusual genomic organization has been conserved in C. briggsae, as shown by cloning the C. briggsae lir-2, lir-1, and lin-26 homologs. Particularly striking is the sequence conservation throughout the first lir-1 intron, which is very long in both species. Structural conservation is functionally meaningful as C. briggsae lin-26 is also expressed in the nonneuronal ectoderm and can complement a C. elegans lin-26 null mutation.</jats:p>",
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