DOI: 10.1093/genetics/151.4.1273. Analysis of Mutations in the Yeast mRNA Decapping Enzyme

Analysis of Mutations in the Yeast mRNA Decapping Enzyme

10.1093/genetics/151.4.1273

Crossref journal-article

Oxford University Press (OUP)

Genetics (286)

Abstract

Abstract A major mechanism of mRNA decay in yeast is initiated by deadenylation, followed by mRNA decapping, which exposes the transcript to 5′ to 3′ exonucleolytic degradation. The decapping enzyme that removes the 5′ cap structure is encoded by the DCP1 gene. To understand the function of the decapping enzyme, we used alanine scanning mutagenesis to create 31 mutant versions of the enzyme, and we examined the effects of the mutations both in vivo and in vitro. Two types of mutations that affected mRNA decapping in vivo were identified, including a temperature-sensitive allele. First, two mutants produced decapping enzymes that were defective for decapping in vitro, suggesting that these mutated residues are required for enzymatic activity. In contrast, several mutants that moderately affected mRNA decapping in vivo yielded decapping enzymes that had at least the same specific activity as the wild-type enzyme in vitro. Combination of alleles within this group yielded decapping enzymes that showed a strong loss of function in vivo, but that still produced fully active enzymes in vitro. This suggested that interactions of the decapping enzyme with other factors may be required for efficient decapping in vivo, and that these particular mutations may be disrupting such interactions. Interestingly, partial loss of decapping activity in vivo led to a defect in normal deadenylation-dependent decapping, but it did not affect the rapid deadenylation-independent decapping triggered by early nonsense codons. This observation suggested that these two types of mRNA decapping differ in their requirements for the decapping enzyme.

Bibliography

Tharun, S., & Parker, R. (1999). Analysis of Mutations in the Yeast mRNA Decapping Enzyme. Genetics, 151(4), 1273â1285.

Authors 2

Sundaresan Tharun (first)
Roy Parker (additional)

References 38 Referenced 60

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Dates

Type	When
Created	4 years, 4 months ago (April 27, 2021, 11:04 p.m.)
Deposited	3 years, 8 months ago (Jan. 3, 2022, 12:48 p.m.)
Indexed	1 year, 3 months ago (May 22, 2024, 9:52 a.m.)
Issued	26 years, 5 months ago (April 1, 1999)
Published	26 years, 5 months ago (April 1, 1999)
Published Online	26 years, 5 months ago (April 1, 1999)
Published Print	26 years, 5 months ago (April 1, 1999)

Funders 0

None

BibTeX

@article{Tharun_1999, title={Analysis of Mutations in the Yeast mRNA Decapping Enzyme}, volume={151}, ISSN={1943-2631}, url={http://dx.doi.org/10.1093/genetics/151.4.1273}, DOI={10.1093/genetics/151.4.1273}, number={4}, journal={Genetics}, publisher={Oxford University Press (OUP)}, author={Tharun, Sundaresan and Parker, Roy}, year={1999}, month=apr, pages={1273–1285} }

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