DOI: 10.1093/genetics/151.2.485. Role of Saccharomyces cerevisiae Chromatin Assembly Factor-I in Repair of Ultraviolet Radiation Damage in Vivo

Role of Saccharomyces cerevisiae Chromatin Assembly Factor-I in Repair of Ultraviolet Radiation Damage in Vivo

10.1093/genetics/151.2.485

Crossref journal-article

Oxford University Press (OUP)

Genetics (286)

Abstract

Abstract In vitro, the protein complex Chromatin Assembly Factor-I (CAF-I) from human or yeast cells deposits histones onto DNA templates after replication. In Saccharomyces cerevisiae, the CAC1, CAC2, and CAC3 genes encode the three CAF-I subunits. Deletion of any of the three CAC genes reduces telomeric gene silencing and confers an increase in sensitivity to killing by ultraviolet (UV) radiation. We used double and triple mutants involving cac1Δ and yeast repair gene mutations to show that deletion of the CAC1 gene increases the UV sensitivity of cells mutant in genes from each of the known DNA repair epistasis groups. For example, double mutants involving cac1Δ and excision repair gene deletions rad1Δ or rad14Δ showed increased UV sensitivity, as did double mutants involving cac1Δ and deletions of members of the RAD51 recombinational repair group. cac1Δ also increased the UV sensitivity of strains with defects in either the error-prone (rev3Δ) or error-free (pol30-46) branches of RAD6-mediated postreplicative DNA repair but did not substantially increase the sensitivity of strains carrying null mutations in the RAD6 or RAD18 genes. Deletion of CAC1 also increased the UV sensitivity and rate of UV-induced mutagenesis in rad5Δ mutants, as has been observed for mutants defective in error-free postreplicative repair. Together, these data suggest that CAF-I has a role in error-free postreplicative damage repair and may also have an auxiliary role in other repair mechanisms. Like the CAC genes, RAD6 is also required for gene silencing at telomeres. We find an increased loss of telomeric gene silencing in rad6Δ cac1Δ and rad18Δ cac1Δ double mutants, suggesting that CAF-I and multiple factors in the postreplicative repair pathway influence chromosome structure.

Bibliography

Game, J. C., & Kaufman, P. D. (1999). Role of Saccharomyces cerevisiae Chromatin Assembly Factor-I in Repair of Ultraviolet Radiation Damage in Vivo. Genetics, 151(2), 485â497.

Authors 2

John C Game (first)
Paul D Kaufman (additional)

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Dates

Type	When
Created	4 years, 4 months ago (April 27, 2021, 11:01 p.m.)
Deposited	3 years, 7 months ago (Jan. 3, 2022, 1:30 p.m.)
Indexed	1 year ago (Aug. 4, 2024, 2:46 a.m.)
Issued	26 years, 6 months ago (Feb. 1, 1999)
Published	26 years, 6 months ago (Feb. 1, 1999)
Published Online	26 years, 6 months ago (Feb. 1, 1999)
Published Print	26 years, 6 months ago (Feb. 1, 1999)

Funders 0

None

BibTeX

@article{Game_1999, title={Role of Saccharomyces cerevisiae Chromatin Assembly Factor-I in Repair of Ultraviolet Radiation Damage in Vivo}, volume={151}, ISSN={1943-2631}, url={http://dx.doi.org/10.1093/genetics/151.2.485}, DOI={10.1093/genetics/151.2.485}, number={2}, journal={Genetics}, publisher={Oxford University Press (OUP)}, author={Game, John C and Kaufman, Paul D}, year={1999}, month=feb, pages={485–497} }

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  "abstract": "<jats:title>Abstract</jats:title>\n               <jats:p>In vitro, the protein complex Chromatin Assembly Factor-I (CAF-I) from human or yeast cells deposits histones onto DNA templates after replication. In Saccharomyces cerevisiae, the CAC1, CAC2, and CAC3 genes encode the three CAF-I subunits. Deletion of any of the three CAC genes reduces telomeric gene silencing and confers an increase in sensitivity to killing by ultraviolet (UV) radiation. We used double and triple mutants involving cac1\u0394 and yeast repair gene mutations to show that deletion of the CAC1 gene increases the UV sensitivity of cells mutant in genes from each of the known DNA repair epistasis groups. For example, double mutants involving cac1\u0394 and excision repair gene deletions rad1\u0394 or rad14\u0394 showed increased UV sensitivity, as did double mutants involving cac1\u0394 and deletions of members of the RAD51 recombinational repair group. cac1\u0394 also increased the UV sensitivity of strains with defects in either the error-prone (rev3\u0394) or error-free (pol30-46) branches of RAD6-mediated postreplicative DNA repair but did not substantially increase the sensitivity of strains carrying null mutations in the RAD6 or RAD18 genes. Deletion of CAC1 also increased the UV sensitivity and rate of UV-induced mutagenesis in rad5\u0394 mutants, as has been observed for mutants defective in error-free postreplicative repair. Together, these data suggest that CAF-I has a role in error-free postreplicative damage repair and may also have an auxiliary role in other repair mechanisms. Like the CAC genes, RAD6 is also required for gene silencing at telomeres. We find an increased loss of telomeric gene silencing in rad6\u0394 cac1\u0394 and rad18\u0394 cac1\u0394 double mutants, suggesting that CAF-I and multiple factors in the postreplicative repair pathway influence chromosome structure.</jats:p>",
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