DOI: 10.1093/genetics/150.4.1429. The Anatomy of a Hypoxic Operator in Saccharomyces cerevisiae

The Anatomy of a Hypoxic Operator in Saccharomyces cerevisiae

10.1093/genetics/150.4.1429

Crossref journal-article

Oxford University Press (OUP)

Genetics (286)

Abstract

Abstract Aerobic repression of the hypoxic genes of Saccharomyces cerevisiae is mediated by the DNA-binding protein Rox1 and the Tup1/Ssn6 general repression complex. To determine the DNA sequence requirements for repression, we carried out a mutational analysis of the consensus Rox1-binding site and an analysis of the arrangement of the Rox1 sites into operators in the hypoxic ANB1 gene. We found that single base pair substitutions in the consensus sequence resulted in lower affinities for Rox1, and the decreased affinity of Rox1 for mutant sites correlated with the ability of these sites to repress expression of the hypoxic ANB1 gene. In addition, there was a general but not complete correlation between the strength of repression of a given hypoxic gene and the compliance of the Rox1 sites in that gene to the consensus sequence. An analysis of the ANB1 operators revealed that the two Rox1 sites within an operator acted synergistically in vivo, but that Rox1 did not bind cooperatively in vitro, suggesting the presence of a higher order repression complex in the cell. In addition, the spacing or helical phasing of the Rox1 sites was not important in repression. The differential repression by the two operators of the ANB1 gene was found to be due partly to the location of the operators and partly to the sequences between the two Rox1-binding sites in each. Finally, while Rox1 repression requires the Tup1/Ssn6 general repression complex and this complex has been proposed to require the aminoterminal regions of histones H3 and H4 for full repression of a number of genes, we found that these regions were dispensable for ANB1 repression and the repression of two other hypoxic genes.

Bibliography

Deckert, J., Torres, A. M. R., Hwang, S. M., Kastaniotis, A. J., & Zitomer, R. S. (1998). The Anatomy of a Hypoxic Operator in Saccharomyces cerevisiae. Genetics, 150(4), 1429â1441.

Authors 5

Jutta Deckert (first)
Ana Maria Rodriguez Torres (additional)
Soo Myung Hwang (additional)
Alexander J Kastaniotis (additional)
Richard S Zitomer (additional)

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Dates

Type	When
Created	4 years, 4 months ago (April 27, 2021, 10:39 p.m.)
Deposited	3 years, 7 months ago (Jan. 3, 2022, 8:57 a.m.)
Indexed	1 year, 3 months ago (May 11, 2024, 3:14 a.m.)
Issued	26 years, 8 months ago (Dec. 1, 1998)
Published	26 years, 8 months ago (Dec. 1, 1998)
Published Online	26 years, 8 months ago (Dec. 1, 1998)
Published Print	26 years, 8 months ago (Dec. 1, 1998)

Funders 0

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BibTeX

@article{Deckert_1998, title={The Anatomy of a Hypoxic Operator in Saccharomyces cerevisiae}, volume={150}, ISSN={1943-2631}, url={http://dx.doi.org/10.1093/genetics/150.4.1429}, DOI={10.1093/genetics/150.4.1429}, number={4}, journal={Genetics}, publisher={Oxford University Press (OUP)}, author={Deckert, Jutta and Torres, Ana Maria Rodriguez and Hwang, Soo Myung and Kastaniotis, Alexander J and Zitomer, Richard S}, year={1998}, month=dec, pages={1429–1441} }

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  "abstract": "<jats:title>Abstract</jats:title>\n               <jats:p>Aerobic repression of the hypoxic genes of Saccharomyces cerevisiae is mediated by the DNA-binding protein Rox1 and the Tup1/Ssn6 general repression complex. To determine the DNA sequence requirements for repression, we carried out a mutational analysis of the consensus Rox1-binding site and an analysis of the arrangement of the Rox1 sites into operators in the hypoxic ANB1 gene. We found that single base pair substitutions in the consensus sequence resulted in lower affinities for Rox1, and the decreased affinity of Rox1 for mutant sites correlated with the ability of these sites to repress expression of the hypoxic ANB1 gene. In addition, there was a general but not complete correlation between the strength of repression of a given hypoxic gene and the compliance of the Rox1 sites in that gene to the consensus sequence. An analysis of the ANB1 operators revealed that the two Rox1 sites within an operator acted synergistically in vivo, but that Rox1 did not bind cooperatively in vitro, suggesting the presence of a higher order repression complex in the cell. In addition, the spacing or helical phasing of the Rox1 sites was not important in repression. The differential repression by the two operators of the ANB1 gene was found to be due partly to the location of the operators and partly to the sequences between the two Rox1-binding sites in each. Finally, while Rox1 repression requires the Tup1/Ssn6 general repression complex and this complex has been proposed to require the aminoterminal regions of histones H3 and H4 for full repression of a number of genes, we found that these regions were dispensable for ANB1 repression and the repression of two other hypoxic genes.</jats:p>",
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