DOI: 10.1093/genetics/150.4.1377. Characterization of Three Related Glucose Repressors and Genes They Regulate in Saccharomyces cerevisiae

Characterization of Three Related Glucose Repressors and Genes They Regulate in Saccharomyces cerevisiae

10.1093/genetics/150.4.1377

Crossref journal-article

Oxford University Press (OUP)

Genetics (286)

Abstract

Abstract Mig1 and Mig2 are proteins with similar zinc fingers that are required for glucose repression of SUC2 expression. Mig1, but not Mig2, is required for repression of some other glucose-repressed genes, including the GAL genes. A second homolog of Mig1, Yer028, appears to be a glucose-dependent transcriptional repressor that binds to the Mig1-binding sites in the SUC2 promoter, but is not involved in glucose repression of SUC2 expression. Despite their functional redundancy, we found several significant differences between Mig1 and Mig2: (1) in the absence of glucose, Mig1, but not Mig2, is inactivated by the Snf1 protein kinase; (2) nuclear localization of Mig1, but not Mig2, is regulated by glucose; (3) expression of MIG1, but not MIG2, is repressed by glucose; and (4) Mig1 and Mig2 bind to similar sites but with different relative affinities. By two approaches, we have identified many genes regulated by Mig1 and Mig2, and confirmed a role for Mig1 and Mig2 in repression of several of them. We found no genes repressed by Yer028. Also, we identified no genes repressed by only Mig1 or Mig2. Thus, Mig1 and Mig2 are redundant glucose repressors of many genes.

Bibliography

Lutfiyya, L. L., Iyer, V. R., DeRisi, J., DeVit, M. J., Brown, P. O., & Johnston, M. (1998). Characterization of Three Related Glucose Repressors and Genes They Regulate in Saccharomyces cerevisiae. Genetics, 150(4), 1377â1391.

Authors 6

Linda L Lutfiyya (first)
Vishwanath R Iyer (additional)
Joe DeRisi (additional)
Michael J DeVit (additional)
Patrick O Brown (additional)
Mark Johnston (additional)

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Dates

Type	When
Created	4 years, 4 months ago (April 27, 2021, 10:39 p.m.)
Deposited	3 years, 7 months ago (Jan. 3, 2022, 8:42 a.m.)
Indexed	5 months, 1 week ago (March 19, 2025, 10:28 a.m.)
Issued	26 years, 8 months ago (Dec. 1, 1998)
Published	26 years, 8 months ago (Dec. 1, 1998)
Published Online	26 years, 8 months ago (Dec. 1, 1998)
Published Print	26 years, 8 months ago (Dec. 1, 1998)

Funders 0

None

BibTeX

@article{Lutfiyya_1998, title={Characterization of Three Related Glucose Repressors and Genes They Regulate in Saccharomyces cerevisiae}, volume={150}, ISSN={1943-2631}, url={http://dx.doi.org/10.1093/genetics/150.4.1377}, DOI={10.1093/genetics/150.4.1377}, number={4}, journal={Genetics}, publisher={Oxford University Press (OUP)}, author={Lutfiyya, Linda L and Iyer, Vishwanath R and DeRisi, Joe and DeVit, Michael J and Brown, Patrick O and Johnston, Mark}, year={1998}, month=dec, pages={1377–1391} }

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