DOI: 10.1093/genetics/150.1.21. The [KIL-d] Cytoplasmic Genetic Element of Yeast Results in Epigenetic Regulation of Viral M Double-Stranded RNA Gene Expression

The [KIL-d] Cytoplasmic Genetic Element of Yeast Results in Epigenetic Regulation of Viral M Double-Stranded RNA Gene Expression

10.1093/genetics/150.1.21

Crossref journal-article

Oxford University Press (OUP)

Genetics (286)

Abstract

Abstract [KIL-d] is a cytoplasmically inherited genetic trait that causes killer virus-infected cells of Saccharomyces cerevisiae to express the normal killer phenotypes in a/α cells, but to show variegated defective killer phenotypes in a or α type cells. Mating of [KIL-d] haploids results in “healing” of their phenotypic defects, while meiosis of the resulting diploids results in “resetting” of the variegated, but mitotically stable, defects. We show that [KIL-d] does not reside on the double-stranded RNA genome of killer virus. Thus, the [KIL-d] effect on viral gene expression is epigenetic in nature. Resetting requires nuclear events of meiosis, since [KIL-d] can be cytoplasmically transmitted during cytoduction without causing defects in killer virus expression. Subsequently, mating of these cytoductants followed by meiosis generates spore clones expressing variegated defective phenotypes. Cytoduction of wild-type cytoplasm into a phenotypically defective [KIL-d] haploid fails to heal, nor does simultaneous or sequential expression of both MAT alleles cause healing. Thus, healing is not triggered by the appearance of heterozygosity at the MAT locus, but rather requires the nuclear fusion events which occur during mating. Therefore, [KIL-d] appears to interact with the nucleus in order to exert its effects on gene expression by the killer virus RNA genome.

Bibliography

TallÃ³czy, Z., Menon, S., Neigeborn, L., & Leibowitz, M. J. (1998). The [KIL-d] Cytoplasmic Genetic Element of Yeast Results in Epigenetic Regulation of Viral M Double-Stranded RNA Gene Expression. Genetics, 150(1), 21â30.

Authors 4

Zsolt Tallóczy (first)
Sujoy Menon (additional)
Lenore Neigeborn (additional)
Michael J Leibowitz (additional)

References 33 Referenced 15

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10.1093/genetics/144.4.1375 / Genetics / Genesis and variability of [PSI] prion factors in Saccharomyces cerevisiae by Derkatch (1996)
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Dates

Type	When
Created	4 years, 4 months ago (April 27, 2021, 10:10 p.m.)
Deposited	3 years, 7 months ago (Jan. 3, 2022, 6:52 a.m.)
Indexed	1 year, 10 months ago (Sept. 30, 2023, 6:20 p.m.)
Issued	26 years, 11 months ago (Sept. 1, 1998)
Published	26 years, 11 months ago (Sept. 1, 1998)
Published Online	26 years, 11 months ago (Sept. 1, 1998)
Published Print	26 years, 11 months ago (Sept. 1, 1998)

Funders 0

None

BibTeX

@article{Tall_czy_1998, title={The [KIL-d] Cytoplasmic Genetic Element of Yeast Results in Epigenetic Regulation of Viral M Double-Stranded RNA Gene Expression}, volume={150}, ISSN={1943-2631}, url={http://dx.doi.org/10.1093/genetics/150.1.21}, DOI={10.1093/genetics/150.1.21}, number={1}, journal={Genetics}, publisher={Oxford University Press (OUP)}, author={Tallóczy, Zsolt and Menon, Sujoy and Neigeborn, Lenore and Leibowitz, Michael J}, year={1998}, month=sep, pages={21–30} }

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