DOI: 10.1093/genetics/149.1.417. Isolation and Characterization of Arabidopsis Mutants Defective in the Induction of Ethylene Biosynthesis by Cytokinin

Isolation and Characterization of Arabidopsis Mutants Defective in the Induction of Ethylene Biosynthesis by Cytokinin

10.1093/genetics/149.1.417

Crossref journal-article

Oxford University Press (OUP)

Genetics (286)

Abstract

Abstract Cytokinins elevate ethylene biosynthesis in etiolated Arabidopsis seedlings via a post-transcriptional modification of one isoform of the key biosynthetic enzyme ACC synthase. In order to begin to dissect the signaling events leading from cytokinin perception to this modification, we have isolated a series of mutants that lack the ethylene-mediated triple response in the presence of cytokinin due to their failure to increase ethylene biosynthesis. Analysis of genetic complementation and mapping revealed that these Cin mutants (cytokinin-insensitive) represent four distinct complementation groups, one of which, cin4, is allelic to the constitutive photomorphogenic mutant fus9/cop10. The Cin mutants have subtle effects on the morphology of adult plants. We further characterized the Cin mutants by analyzing ethylene biosynthesis in response to various other inducers and in adult tissues, as well as by assaying additional cytokinin responses. The cin3 mutant did not disrupt ethylene biosynthesis under any other conditions, nor did it disrupt any other cytokinin responses. Only cin2 disrupted ethylene biosynthesis in multiple circumstances. cin1 and cin2 made less anthocyanin in response to cytokinin. cin1 also displayed reduced shoot initiation in tissue culture in response to cytokinin, suggesting that it affects a cytokinin signaling element.

Bibliography

Vogel, J. P., Schuerman, P., Woeste, K., Brandstatter, I., & Kieber, J. J. (1998). Isolation and Characterization of Arabidopsis Mutants Defective in the Induction of Ethylene Biosynthesis by Cytokinin. Genetics, 149(1), 417â427.

Authors 5

John P Vogel (first)
Peter Schuerman (additional)
Keith Woeste (additional)
Ingrid Brandstatter (additional)
Joseph J Kieber (additional)

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Dates

Type	When
Created	4 years, 6 months ago (Feb. 12, 2021, 5:30 p.m.)
Deposited	3 years, 8 months ago (Jan. 3, 2022, 4:32 a.m.)
Indexed	4 weeks ago (Aug. 7, 2025, 4:51 p.m.)
Issued	27 years, 4 months ago (May 1, 1998)
Published	27 years, 4 months ago (May 1, 1998)
Published Online	27 years, 4 months ago (May 1, 1998)
Published Print	27 years, 4 months ago (May 1, 1998)

Funders 0

None

BibTeX

@article{Vogel_1998, title={Isolation and Characterization of Arabidopsis Mutants Defective in the Induction of Ethylene Biosynthesis by Cytokinin}, volume={149}, ISSN={1943-2631}, url={http://dx.doi.org/10.1093/genetics/149.1.417}, DOI={10.1093/genetics/149.1.417}, number={1}, journal={Genetics}, publisher={Oxford University Press (OUP)}, author={Vogel, John P and Schuerman, Peter and Woeste, Keith and Brandstatter, Ingrid and Kieber, Joseph J}, year={1998}, month=may, pages={417–427} }

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