DOI: 10.1093/genetics/143.3.1115. Fine-Structure Mapping of Meiosis-Specific Double-Strand DNA Breaks at a Recombination Hotspot Associated With an Insertion of Telomeric Sequences Upstream of the <i>HIS4</i> Locus in Yeast

Fine-Structure Mapping of Meiosis-Specific Double-Strand DNA Breaks at a Recombination Hotspot Associated With an Insertion of Telomeric Sequences Upstream of the HIS4 Locus in Yeast

10.1093/genetics/143.3.1115

Crossref journal-article

Oxford University Press (OUP)

Genetics (286)

Abstract

Abstract Meiotic recombination in Saccharomyces cerevisiae is initiated by double-strand DNA breaks (DSBs). Using two approaches, we mapped the position of DSBs associated with a recombination hotspot created by insertion of telomeric sequences into the region upstream of HIS4. We found that the breaks have no obvious sequence specificity and localize to a region of ~50 bp adjacent to the telomeric insertion. By mapping the breaks and by studies of the exonuclease III sensitivity of the broken ends, we conclude that most of the broken DNA molecules have blunt ends with 3′-hydroxyl groups.

Bibliography

Xu, F., & Petes, T. D. (1996). Fine-Structure Mapping of Meiosis-Specific Double-Strand DNA Breaks at a Recombination Hotspot Associated With an Insertion of Telomeric Sequences Upstream of the HIS4 Locus in Yeast. Genetics, 143(3), 1115â1125.

Authors 2

Fei Xu (first)
Thomas D Petes (additional)

References 0 Referenced 38

None

Dates

Type	When
Created	4 years, 7 months ago (Jan. 13, 2021, 5:02 p.m.)
Deposited	4 years, 4 months ago (April 22, 2021, 4:05 p.m.)
Indexed	1 month, 4 weeks ago (July 2, 2025, 3:11 p.m.)
Issued	29 years, 1 month ago (July 1, 1996)
Published	29 years, 1 month ago (July 1, 1996)
Published Online	29 years, 1 month ago (July 1, 1996)
Published Print	29 years, 1 month ago (July 1, 1996)

Funders 0

None

BibTeX

@article{Xu_1996, title={Fine-Structure Mapping of Meiosis-Specific Double-Strand DNA Breaks at a Recombination Hotspot Associated With an Insertion of Telomeric Sequences Upstream of the HIS4 Locus in Yeast}, volume={143}, ISSN={1943-2631}, url={http://dx.doi.org/10.1093/genetics/143.3.1115}, DOI={10.1093/genetics/143.3.1115}, number={3}, journal={Genetics}, publisher={Oxford University Press (OUP)}, author={Xu, Fei and Petes, Thomas D}, year={1996}, month=jul, pages={1115–1125} }

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