DOI: 10.1093/genetics/134.4.1003. Second-site suppressors of a cold-sensitive external scaffolding protein of bacteriophage phi X174.

Second-site suppressors of a cold-sensitive external scaffolding protein of bacteriophage phi X174.

10.1093/genetics/134.4.1003

Crossref journal-article

Oxford University Press (OUP)

Genetics (286)

Abstract

Abstract This report describes the isolation and characterization of second-site suppressors of a cold-sensitive (cs) external scaffolding protein, gpD, of bacteriophage phi X174. Seven genetically distinct suppressors were isolated. Six of them are located in gene F which encodes the major coat protein of the virus. The seventh is located in gene J which encodes the DNA-binding protein. A subset of the suppressors are trans-acting. These second-site suppressors do not exhibit allele specificity; they are able to suppress defects associated with a csD protein for which they were not selected. The initial characterization of the second-site suppressors and their locations within the major coat protein suggest that the mechanism of suppression may involve both structural and stoichiometric phenomena.

Bibliography

Fane, B. A., Shien, S., & Hayashi, M. (1993). Second-site suppressors of a cold-sensitive external scaffolding protein of bacteriophage phi X174. Genetics, 134(4), 1003â1011.

Authors 3

B A Fane (first)
S Shien (additional)
M Hayashi (additional)

References 0 Referenced 35

None

Dates

Type	When
Created	4 years, 7 months ago (Jan. 13, 2021, 3:20 p.m.)
Deposited	4 years, 4 months ago (April 22, 2021, 3:23 p.m.)
Indexed	1 year, 2 months ago (June 30, 2024, 4:25 a.m.)
Issued	32 years ago (Aug. 1, 1993)
Published	32 years ago (Aug. 1, 1993)
Published Online	32 years ago (Aug. 1, 1993)
Published Print	32 years ago (Aug. 1, 1993)

Funders 0

None

BibTeX

@article{Fane_1993, title={Second-site suppressors of a cold-sensitive external scaffolding protein of bacteriophage phi X174.}, volume={134}, ISSN={1943-2631}, url={http://dx.doi.org/10.1093/genetics/134.4.1003}, DOI={10.1093/genetics/134.4.1003}, number={4}, journal={Genetics}, publisher={Oxford University Press (OUP)}, author={Fane, B A and Shien, S and Hayashi, M}, year={1993}, month=aug, pages={1003–1011} }

JSON

{
  "indexed": {
    "date-parts": [
      [
        2024,
        6,
        30
      ]
    ],
    "date-time": "2024-06-30T08:25:10Z",
    "timestamp": 1719735910866
  },
  "reference-count": 0,
  "publisher": "Oxford University Press (OUP)",
  "issue": "4",
  "license": [
    {
      "start": {
        "date-parts": [
          [
            1993,
            8,
            1
          ]
        ],
        "date-time": "1993-08-01T00:00:00Z",
        "timestamp": 744163200000
      },
      "content-version": "vor",
      "delay-in-days": 0,
      "URL": "https://academic.oup.com/journals/pages/open_access/funder_policies/chorus/standard_publication_model"
    }
  ],
  "content-domain": {
    "domain": [],
    "crossmark-restriction": false
  },
  "published-print": {
    "date-parts": [
      [
        1993,
        8,
        1
      ]
    ]
  },
  "abstract": "<jats:title>Abstract</jats:title>\n               <jats:p>This report describes the isolation and characterization of second-site suppressors of a cold-sensitive (cs) external scaffolding protein, gpD, of bacteriophage phi X174. Seven genetically distinct suppressors were isolated. Six of them are located in gene F which encodes the major coat protein of the virus. The seventh is located in gene J which encodes the DNA-binding protein. A subset of the suppressors are trans-acting. These second-site suppressors do not exhibit allele specificity; they are able to suppress defects associated with a csD protein for which they were not selected. The initial characterization of the second-site suppressors and their locations within the major coat protein suggest that the mechanism of suppression may involve both structural and stoichiometric phenomena.</jats:p>",
  "DOI": "10.1093/genetics/134.4.1003",
  "type": "journal-article",
  "created": {
    "date-parts": [
      [
        2021,
        1,
        13
      ]
    ],
    "date-time": "2021-01-13T20:20:22Z",
    "timestamp": 1610569222000
  },
  "page": "1003-1011",
  "source": "Crossref",
  "is-referenced-by-count": 35,
  "title": "Second-site suppressors of a cold-sensitive external scaffolding protein of bacteriophage phi X174.",
  "prefix": "10.1093",
  "volume": "134",
  "author": [
    {
      "given": "B A",
      "family": "Fane",
      "sequence": "first",
      "affiliation": []
    },
    {
      "given": "S",
      "family": "Shien",
      "sequence": "additional",
      "affiliation": []
    },
    {
      "given": "M",
      "family": "Hayashi",
      "sequence": "additional",
      "affiliation": []
    }
  ],
  "member": "286",
  "published-online": {
    "date-parts": [
      [
        1993,
        8,
        1
      ]
    ]
  },
  "container-title": "Genetics",
  "original-title": [],
  "language": "en",
  "link": [
    {
      "URL": "http://academic.oup.com/genetics/article-pdf/134/4/1003/34593190/genetics1003.pdf",
      "content-type": "application/pdf",
      "content-version": "vor",
      "intended-application": "syndication"
    },
    {
      "URL": "http://academic.oup.com/genetics/article-pdf/134/4/1003/34593190/genetics1003.pdf",
      "content-type": "unspecified",
      "content-version": "vor",
      "intended-application": "similarity-checking"
    }
  ],
  "deposited": {
    "date-parts": [
      [
        2021,
        4,
        22
      ]
    ],
    "date-time": "2021-04-22T19:23:16Z",
    "timestamp": 1619119396000
  },
  "score": 1,
  "resource": {
    "primary": {
      "URL": "https://academic.oup.com/genetics/article/134/4/1003/6011182"
    }
  },
  "subtitle": [],
  "short-title": [],
  "issued": {
    "date-parts": [
      [
        1993,
        8,
        1
      ]
    ]
  },
  "references-count": 0,
  "journal-issue": {
    "issue": "4",
    "published-print": {
      "date-parts": [
        [
          1993,
          8,
          1
        ]
      ]
    }
  },
  "URL": "http://dx.doi.org/10.1093/genetics/134.4.1003",
  "relation": {},
  "ISSN": [
    "1943-2631"
  ],
  "subject": [],
  "published-other": {
    "date-parts": [
      [
        1993,
        8,
        1
      ]
    ]
  },
  "published": {
    "date-parts": [
      [
        1993,
        8,
        1
      ]
    ]
  }
}